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2
Maintenance Schedule
Weekly Maintenance
Weekly Maintenance
Quarterly Maintenance
28
EASY-nLC 1200 Troubleshooting & Maintenance Guide
❖ To check the solvent levels
Note The instruments reminds the user if the solvents have not been refreshed within
the last 14 days. It is particularly recommended to refresh solvent B regularly for
stable concentrations.
1. Visually inspect solvent bottles A and B, and the autosampler bottles W3, as well as W1
and 2, if used. Refill if necessary but do not top-up.
Tip You can also inspect the purity of the solvent to ensure no visible precipitates have
formed that might lead to blockages in the pump line.
2. Visually inspect the autosampler wash bottle in position W4 and the plastic waste beaker
in front of the solvent system compartment, and empty if necessary.
❖ To draw fresh solvent into the system and to flush air out of the system
Run the Purge Solvent scripts with two purge iterations, and run the Flush Air script
until flush volumes fall below 12 µL.
Because the vapor pressures of formic acid, water, and acetonitrile differ, the solvent
composition changes over time. Refill the solvent bottles to maintain a consistent solvent
composition. Discard the old solvents and fill the bottles with new, do not top up.
Exchange solvent B mixture (max. 95% ACN) instead of filling up to maintain a defined
composition.
A solvent refresh due reminder is shown 14 days after the last solvent refresh acknowledge.
Perform the following procedures quarterly (every 3 months).
❖ To check the backpressure
To test for system blockages, run the Back Pressure script for both solvents (see
Pressure"
on
page
47).
IMPORTANT The script evaluates the backpressure based on using 99.9% water for
solvent A and 80% acetonitrile for solvent B.
❖ To check the cooler temperature
Check that the actual plate temperature matches its set point (readout on the Home >
Overview page). Allowed maximum deviation from set point temperature is +/- 0,5°C.
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Troubleshooting
