Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 192

7
Troubleshooting
Using the Direct Controls for Troubleshooting and Maintenance
Using the Direct Controls To Fill or Empty Pump S
170 EASY-nLC 1200 Troubleshooting & Maintenance Guide
Before using the direct controls to fill or empty pump S, make sure that the autosampler
needle is inserted into the appropriate wash or waste bottle.
IMPORTANT To avoid contaminating pump S, take care to place the autosampler needle
in the appropriate wash bottle before filling the pump.
❖ To fill pump S
1. Make sure that a wash bottle with the appropriate solvent is installed in position W3.
2. Send the autosampler needle to the appropriate position as follows:
a. On the Home > Overview page, press the autosampler icon.
The Autosampler dialog box opens (see
b. Press W3.
The autosampler needle moves to the W3 position.
c. Press Needle Down.
The autosampler needle descends into the W3 bottle.
d. Press Close to close the Autosampler dialog box.
3. Draw solvent into pump S as follows:
a. Press the pump S icon.
The Pump S dialog box opens.
b. In the flow rate box, enter the filling flow rate: –0.01 to –300 µL/min.
For best results, enter a flow rate of –40 µL/min to fill pump S.
c. In the volume box, enter the volume that you want pump S to draw.
d. Press Start.
The pump draws solvent until it draws the requested volume or reaches the full
position, whichever occurs first.
CAUTION To equalize the pressure, leave the needle for at least 30 seconds after the
completed refill in the W3 bottle. Otherwise air can be pulled into the needle.
❖ To empty pump S to waste
1. Make sure that a wash bottle with a needle wash insert is installed in position W4.
2. Send the autosampler needle to the appropriate position as follows:
a. On the Home > Overview page, press the autosampler icon.
The Autosampler dialog box opens (see
Figure 141 on page 172).
Figure 141 on page 172).
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