Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 243

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g. Press Start.
The script runs until the flush threshold reaches the specified value or one of these
error messages appears on the Output page:
• Aborting unable to build pressure
• Aborting check solvent level
3. Depending on whether the Flush Air script passed or failed, do one of the following:
• If the Flush Air script failed, leave the Maintenance > Scripts page open and go to
step 4 to determine if the autosampler needle is blocked.
• If the Flush Air script passed, leave the Maintenance > Scripts page open and go to
step 5 to determine if the autosampler requires calibration.
4. To determine if the autosampler needle is blocked, do the following:
a. Disconnect the PEEKsil autosampler needle from port 1 of valve S.
b. Disconnect the waste line from port 3 of valve W and connect it to port 1 of valve S.
c. Place the free end of the waste line in an HPLC vial filled with solvent A.
d. To open the Purge script, select Purge in the Name list.
e. Select the Purge Pump S check box.
f. Press Start.
g. Verify that pump S aspirates solvent from the HPLC vial.
h. Do one of the following:
• If pump S can aspirate solvent through the waste tubing, the needle is blocked.
Reconnect the PEEK waste tubing to port 3 of valve W and replace the
autosampler needle (see
• If pump S cannot aspirate solvent through the waste tubing, go to
"Troubleshooting a Pump That Fails the Flush Air Script"
5. To determine if the autosampler needle is going to the correct position and depth, go to
the next procedure, "Checking Sample Pickup."
Troubleshooting the Autosampler Aspiration
"Replacing the Autosampler Needle"
EASY-nLC 1200 Troubleshooting & Maintenance Guide
7
Troubleshooting
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