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Product Manual for IonPac CS16
6.1.
High Back Pressure
6.1.1.
Finding the Source of High System Pressure
Total system pressure for the IonPac CG16 Guard Column plus the CS16 Analytical Column when using the test
chromatogram conditions should be equal or less than 1,850 psi (12.75 MPa). If the system pressure is higher than 1,950 psi
(13.44 MPa), it is advisable to determine the cause of the high system pressure. The system should be operated with a High-
Pressure In-Line Filter (P/N 044105) which is positioned between the Gradient Pump pressure transducer and the injection
valve. Make sure you have one in place and that it is not contaminated. The CTC-1, if required, should be installed after the
High Pressure In-Line Filter.
A. Make sure that the pump is set to the correct eluent flow rate. Higher than recommended eluent flow rates will
cause higher pressure. Measure the pump flow rate if necessary with a stop watch and graduated cylinder.
B. Determine which part of the system is causing the high pressure. High pressure could be due to a plugged tubing
or tubing with collapsed walls, an injection valve with a clogged port, a column with particulates clogging the bed
support, a clogged High-Pressure In-Line Filter, suppressor or the detector cell.
To determine which part of the chromatographic system is causing the problem, disconnect the pump eluent line
from the injection valve and turn the pump on. Watch the pressure; it should not exceed 50 psi (0.34 MPa). Continue
adding system components (injection valve, column(s), suppressor and detector) one by one, while monitoring the
system pressure. The pressure should increase up to a maximum when the Guard and Analytical columns are
connected (see Table 9, "Typical CS16/CG16 Operating Back Pressures").
The Cation Self-Regenerating Suppressor 300 may add up to 100 psi (0.69 MPa) of back pressure. No other
components should add more than 100 psi (0.69 MPa) of pressure. Refer to the appropriate manual for cleanup or
replacement of the problem component.
Column
CS16 5-mm Analytical
CG16 5-mm Guard
CS16 + CG16 5-mm columns
CS16 3-mm Analytical
CG16 3-mm Guard
CS16 + CG16 3-mm columns
CS16 0.5-mm Capillary
CG16 0.5-mm Capillary Guard
CS16 + CG16 0.5-mm columns
Document No. 031747-05
Table 9
Typical CS16/CG16 Operating Back Pressures
Typical Back Pressure
Psi (MPa)
< 1,400 (9.65)
< 450 (3.10)
< 1,850 (12.75)
< 1,400 (9.65)
< 450 (3.10)
< 1,850 (12.75)
< 1,400 (9.65)
< 450 (3.10)
< 1,850 (12.75)
© 2010 Dionex Corporation
Page 51 of 65
Flow Rate
mL/min
1.0
1.0
1.0
0.36
0.36
0.36
0.01
0.01
0.01
December 2010
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