Spray Issues - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

Spray Issues

Table 21. Possible causes of an unstable spray
Symptom
Unstable spray
Thermo Scientific
Table 21 lists possible causes of an unstable spray.
Possible cause
Post-column outgassing
Poor connection in either the
liquid junction or the
DirectJunction
Incorrect MS settings or emitter
position
Dirty emitter
Poor high-voltage connection
Air movement from an air
conditioning vent
Incorrect sleeve
Action
Degas the solvents.
Check all of the connections.
Optimize the MS tune file for the desired
flow rate and the emitter position.
Clean the emitter with an organic solvent
such as methanol, acetonitrile, or ethanol.
Use only LC/MS-grade solvents.
Check all of the high-voltage connections.
Reconnect as necessary.
Deflect the air from the vent, or use a shield
to protect the ion source.
Refer to the complete Nano LC Connections
Guide, available here:
https://tools.thermofisher.com/content/sfs/
manuals/PP-64583-LC-MS-NanoLC-Comp
lete-Easy-Guide-PP64583-EN.pdf
EASY-nLC 1200 Troubleshooting & Maintenance Guide
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