Sample Pickup - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

3
Maintenance Scripts and Service Records
Maintenance Scripts

Sample Pickup

42 EASY-nLC 1200 Troubleshooting & Maintenance Guide
The EASY-nLC instrument sends a contact closure signal to the mass spectrometer.
The contact closure signal triggers the mass spectrometer to start scanning. The Scan
LED on the front panel of a Thermo Scientific mass spectrometer flashes blue when
the contact closure signal is set up correctly.
The Sample Pickup script tests the accuracy of the autosampler pickup function. During the
script, the XYZ robotic arm moves to the specified sample location. Then pump S aspirates
the specified volume from the vial or microplate well and ejects this volume to waste. To
determine if the autosampler is aspirating the specified sample volume, weigh the sample both
before and after you run the test script as described in
page 222.
Use this script to verify the calibration of the XYZ robotic arm and to determine the
appropriate flow rate (aspiration rate by pump S) setting in the method (or Xcalibur
instrument method) that you plan to use for your samples. The appropriate flow rate depends
on the sample viscosity.
Figure 30 shows the parameters for the Sample Pickup script and the Position dialog box that
opens when you press the value box for the Position parameter.
Figure 30. Sample Pickup script parameters
"Checking Sample Pickup" on
Thermo Scientific