Troubleshooting A System Blockage - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

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Troubleshooting

Troubleshooting a System Blockage

Troubleshooting a System Blockage
208 EASY-nLC 1200 Troubleshooting & Maintenance Guide
Use this troubleshooting procedure when the backpressure for a chromatographic method
rises above the expected level or when the flow decreases below the set rate for the column
equilibration or sample loading steps.
In most cases, an increase in backpressure is caused by a blocked column or a blocked Column
Out line. The Column Out line has an ID of 20 µm for the EASY-nLC 1200 instrument and
is the smallest ID line that the sample passes through, from sample pickup to the columns.
Troubleshooting a system blockage requires these tools and materials:
Tools
CAUTION Wear powder-free gloves and safety glasses when handling parts of the LC system
that come into contact with solvents.
When running the Back Pressure script, the system also checks for an unusually low
backpressure. If the backpressure is unusually low, the system probably has a leak.
To troubleshoot a system blockage, follow these procedures:
1. "Running the Back Pressure Test for the A and B Solvent Paths,"
2. Depending on the test results, replace the blocked components in the column assembly or
follow one or both of these procedures:
• "Troubleshooting a System Blockage when Test Solvent A Fails"
• "Troubleshooting a System Blockage when Test Solvent B Fails"
Parts and materials
• Powder-free gloves
• EASY-nLC 1200 instrument: Viper union, P/N SC900
next procedure
on page 210
on page 216
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