Checking Sample Pickup - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

7
Troubleshooting

Checking Sample Pickup

Checking Sample Pickup
Figure 165. Sample Pickup script under the Maintenance menu tab
222 EASY-nLC 1200 Troubleshooting & Maintenance Guide
When you run the Sample Pickup script, the autosampler withdraws a specified volume from
a specified position. To verify the volume withdrawn by the autosampler, place a known
volume into the specified position, and measure the remaining volume after you run the
script. You can use a pipette or an analytical balance with microgram accuracy to determine
the initial and final volumes.
❖ To check sample pickup from a microtiter plate or vial
1. Fill the well or vial with 12 µL of solvent A.
2. Weigh the microwell plate or vial.
3. Insert the tray into the tray compartment.
4. Press Maintenance > Scripts.
5. Run the Sample Pickup script with a pickup volume of 10 µL and your default flow
(see Figure 165).
6. After the EASY-nLC application finishes the script, do the following:
a. Eject the tray.
b. Check that 2 µL is left in the well or vial by reweighing the microplate or vial or by
checking the volume with a pipette.
c. If the result is different from 2 µL, check the needle for any damages, recalibrate the
autosampler, run the Flush Air script, and then rerun the sample pickup check.
For information about calibrating the autosampler, see
about the Flush Air script, see
7. Repeat the above steps using lower volumes:
"Flush Air" on page 33.
Chapter 8. For information
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