Sample Signal Weak Or Absent - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

7
Troubleshooting
Troubleshooting Tips

Sample Signal Weak or Absent

Table 17. Possible causes of a weak or absent signal
Symptom
The sample signal is missing or
weak.
144 EASY-nLC 1200 Troubleshooting & Maintenance Guide
If the sample signal is missing or weak, review the troubleshooting tips in
Possible cause
Sample not aspirated
(See Figure 123
Damaged or incorrect column
(See Figure 124
Leak in the system
(See Figure 125
Incorrect column configuration Check the column configuration.
Incorrect solvent in W3 bottle
Incorrect sample loop
configuration
Action
Run the Sample Pickup script. If the Sample
on page 145.) Pickup script fails, see
Autosampler Aspiration"
Make sure that you have installed the correct
on page 146.) column or columns.
Check the backpressure on the columns by
running the column equilibration scripts.
Run the Leaks test on the system.
on page 147.)
If the test fails, see
of the System Leak Test"
1. Press Maintenance > Devices.
2. In the Devices list, select EASY-nLC
(HPLC).
3. Press the Properties tab.
4. Clear the One Column Setup check box for
a two-column setup or select this check box
for a one-column setup.
For more information, refer to the EASY-nLC
Series Getting Started Guide.
Make sure that the W3 bottle contains solvent A.
Check the loop configuration on the Properties
page for the EASY-nLC HPLC device on the
Maintenance > Devices page.
Make sure that the configured loop volume
matches the installed sample loop.
Table 17.
"Troubleshooting the
on page 220.
"Troubleshooting the Results
on page 183.
Thermo Scientific