Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 106

5
Routine Maintenance
Replacing Viper/nanoViper solvent lines
84 Troubleshooting & Maintenance Guide
Figure 65. nanoViper fitting
PEEK sealing surface
Screw
Even though nanoViper fittings can withstand UHPLC backpressures of up to 1200 bar
(17404 psi), they are fingertight fittings, which require only very small torques to seal.
Therefore, you must follow the guidelines below to avoid damage by overtightening.
❖ To use a nanoViper fitting
1. Insert the nanoViper fitting into the target port and slowly rotate the screw clockwise
until you feel resistance.
2. Turn the screw clockwise to an angle between 0 and 45 degrees (1/8-turn) using the black
knurled tightening tool.
3. Run the Leaks script for the system as described in
page 178.
When the leak test ends, the system is at atmospheric pressure.
IMPORTANT To extend the lifetime of the nanoViper fittings, open and close
connections at atmospheric system pressures only. Opening and closing connections
at high system pressures can reduce the lifetime of the fitting system.
4. If the Leaks script fails because the new connection is not leak tight, use the black knurled
tightening tool to turn the screw by as much as an additional 45 degrees. Do not turn the
screw beyond an angle of 90 degrees from where you felt the initial resistance.
IMPORTANT To prevent damage to the sealing surface of the nanoViper fitting, take
care not to overtighten the nanoViper fitting.
Removable, black
knurled tightening tool
"Running a System Leak Test" on
Thermo Scientific