Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 78

3
Maintenance Scripts and Service Records
Maintenance Scripts
56 EASY-nLC 1200 Troubleshooting & Maintenance Guide
❖ To run the Direct Infusion script
1. Select the Direct Infusion script as follows:
a. Press Maintenance > Scripts.
b. In the Category list, select Calibrate.
c. In the Name list, select Direct Infusion.
2. Set up the parameters for this script as follows:
a. Press the Parameters tab.
b. In the Pickup Volume [µL] box, enter the volume that you want the autosampler
needle to pick up. The autosampler transfers this volume into the sample loop.
c. In the Pickup Flow [µL/min] box, enter the flow rate at which pump S aspirates the
sample from the vial or well.
d. In the Pickup Position box, enter the vial or well position.
e. In Infusion Volume box [µL], enter the volume that pump A dispenses to deliver the
sample from the sample loop to the Column out line, to the emitter.
f. In the Infusion Flow [µL/min] box, enter the flow rate at which pump A delivers the
sample to the emitter.
g. In the Wash option box select which Wash bottle (W1, W2 or W3) is used in a Wash
step after pump A has dispensed the specified volume of solvent A. Select Off to skip
the Wash step.
During the Wash step, pump S delivers 100 µL of solventW3 through the loop and
needle to the waste bottle. Afterwards, the loop and the needle are washed with the
solvent from the selected Wash bottle (W1, W2, or W3). Then, the entire fluid path
is purged with solventW3.
3. Press Start.
IMPORTANT The Direct Infusion script flushes the solvent lines before it ends.
Stopping the script before it ends can leave sample solution in the flow path.
4. If you press Stop before the script ends, follow the instructions in message box that
appears to make sure that the current sample is flushed from the system (see Figure 42).
Thermo Scientific