Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 57

Thermo Scientific
❖ To run the Precolumn Equilibration script
1. Open the Precolumn Equilibration script as follows:
a. Press Maintenance > Scripts.
b. In the Category list, select Prepare.
c. In the Name list, select Precolumn Equilibration.
2. Set up the parameters for this script as follows:
a. Press the Parameters tab.
b. Enter the appropriate values:
• In the Volume [µL] box, enter the volume of solvent A to use to equilibrate the
precolumn.
For best results, use at least 10 column volumes to equilibrate the precolumn.
• In the Flow [µL/min] box, enter the flow rate for the precolumn equilibration
step.
If the flow field is left empty, the pump operates at the set pressure.
• In the Max Pressure [bar] box, enter the maximum allowed pressure.
If the pressure field is left empty, the pump operates at the set flow (as long as it is
below the instrument maximum pressure of 1180 bar). If both a flow and a max
pressure are specified, the pump flow is limited by whichever parameter is
reached first.
CAUTION Check the maximum pressure rating for the precolumn. Running
the instrument at pressures higher than the column's maximum pressure
rating reduces the column lifespan.
3. Press Start.
3
Maintenance Scripts and Service Records
EASY-nLC 1200 Troubleshooting & Maintenance Guide
Maintenance Scripts
35