Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 178

7
Troubleshooting
Troubleshooting Tips
156 EASY-nLC 1200 Troubleshooting & Maintenance Guide
Figure 134 shows the effect of a high-organic hold at the end of the gradient run on pressure.
The pressure continues to drop until the column equilibrates to the high-organic content.
Figure 134. Gradient run with an adequate high-organic hold at the end of the run
The gradient reaches
100% solvent B.
The column is full of
high-organic.
The pressure begins
to drop when the
high-organic mobile
phase reaches the
column.
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