Using Nanoviper Fittings Quick Reference Guide; Connecting Nanoviper Fittings - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

Using nanoViper Fittings Quick Reference Guide

Most of the plumbing connections use nanoViper fittings (see
Figure 1.
PEEK
sealing
surface
Even though nanoViper fittings can withstand UHPLC backpressures of up to ~1200 bar (~17 404 psi),
they are fingertight fittings, which require only very small torques to seal. To avoid damage by
overtightening, follow the instructions in this guide.
Contents
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Connecting
❖ To connect a nanoViper fitting to a receiving port
nanoViper
1. Insert the nanoViper fitting into the receiving port. Then turn the screw clockwise until you feel
Fittings
resistance.
2. Turn the screw clockwise to an angle between 0 and 45 degrees (1/8-turn) using the black knurled
nut.
3. Run the system leak test (see
When the leak test ends, the system is at atmospheric pressure.
4. If the Leaks script fails because the new connection is not leak tight, use the black knurled nut to turn
the screw up to an additional 45 degrees. Do not turn the screw beyond an angle of 90 degrees from
where you felt the initial resistance.
nanoViper fitting
Screw

Connecting nanoViper Fittings

nanoViper Tubing Connections
Returning the System to Atmospheric Pressure
IMPORTANT To extend the lifetime of the nanoViper fittings, open and close connections at
atmospheric system pressures only. Opening and closing connections at high system pressures can
reduce the lifetime of the fitting system.
IMPORTANT To prevent damage to the sealing surface of the nanoViper fitting, take care not to
overtighten the fingertight nanoViper fitting.
Removable knurled nut
"Running a System Leak Test"
273
Figure 1).
on page 178).