Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 264

8
Calibrating the Autosampler's XYZ Robot
Calibrating the Wash Bottle and Extra Vial Positions
242 EASY-nLC 1200 Troubleshooting & Maintenance Guide
4. Center the needle above the bottle as follows:
• Press the Needle In/Out (2) buttons to adjust the x-axis position.
• Press the Needle Left/Right (3) buttons to adjust the y-axis position.
y axis
x axis
5. Using the step buttons in the Needle Up/Down area (5), adjust the needle height as
follows:
a. Use the double step button until the needle reaches the bottom - identified as the O
ring moves.
b. Use 2 double steps to lift the needle up.
Note The O-ring at the top of the needle holder rises a little when the needle
reaches the bottom of the well.
c. Use single steps to move needle down and accurately reach the bottom.
d. Use 2 double steps and then 2 steps to move the needle up.
Two large steps higher than the bottom of the wash bottle insert is the appropriate needle
height.
6. Press Capture when you are satisfied with the current xyz-coordinate for position W4.
The color of the bottle container number changes from green to red.
7. (Optional) To calibrate the extra vial positions for the 6 × 8 plate format, do the
following:
a. Press V1 vial (5) to select it.
b. Repeat step 4 through
8. Press Save and then press Close to close the calibration dialog box.
Right
step 6 for vial V1.
In
Out
Left
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