Preparing The Autosampler For The Calibration Routines - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

Preparing the Autosampler for the Calibration Routines

Thermo Scientific
To prepare the autosampler for the calibration routines, you must remove the left side panel
from the instrument and load the appropriate adapter plate, vials or microtiter plate, wash
bottle (W4) with insert, and (optional) extra vial into the tray compartment.
CAUTION Calibrating the autosampler requires removal of the side plate and visual
inspection of the autosampler needle holder. Because the autosampler compartment
contains moving parts and sharp needles, make sure to keep hands clear when operating
the autosampler during calibration.
❖ To prepare the autosampler for the plate calibration routine
1. Install the appropriate adapter plate.
2. To calibrate the vial or well positions on a plate, do one of the following:
• To calibrate the 6 × 8 vial positions, remove the vial caps from two vials and load
them into the adapter plate in positions A1 and F8.
• To calibrate a microwell plate, remove the mat or cover from the microwell plate and
load the microwell plate onto the adapter plate.
3. Open the Tools view for the autosampler as follows:
a. Press Maintenance > Devices.
b. In the Devices list, select the autosampler.
c. Press the Tools tab.
4. In the Plate area, do the following:
a. Select the appropriate plate format from the list, or create a new plate format
(To create and select plate formats, see
b. Press Use.
5. Remove the left side panel so that you can watch the movement of the needle.
To calibrate the plate, see
❖ To prepare the autosampler for the V1 and W4 calibration routine
1. Place an empty, uncapped vial in position V1.
2. Ensure a wash bottle is placed in position W4 (used for ejecting waste and cleaning the
outside of the injection needle).
3. For information about preparing the W4 wash bottle, refer to the EASY-nLC Series
Getting Started Guide.
"Managing Plate Formats"
"Calibrating Plates."
EASY-nLC 1200 Troubleshooting & Maintenance Guide
8
Calibrating the Autosampler's XYZ Robot
Preparing the Autosampler for the Calibration Routines
on page 231).
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