Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 220

7
Troubleshooting
Troubleshooting the Results of the System Leak Test
198 EASY-nLC 1200 Troubleshooting & Maintenance Guide
❖ To locate leaks between the flow sensors and valve S
1. To ensure a leak-tight connection to port 4 of valve S, do the following:
a. Disconnect and then reconnect the nanoViper fitting to port 4 of valve S.
b. From the Maintenance > Scripts page, start the Analytical Column Equilibration
script with the maximum pressure set to 1180 bar and the volume set to 140 µL.
For information about setting up the Analytical Column Equilibration script, see
step 22 on page
When you start the Analytical Column Equilibration script, the instrument sets the
rotary valves to these positions and begins pumping solvent through flow sensor A:
• Valve A: 1–6
• Valve B: Center
• Valve S: 1–2
• Valve W: 1–2
c. On the Home > Overview page, check the readback for flow sensor A. Then,
depending on the flow rate, do one of the following:
• If the flow rate is <100 nL/min, you have remedied the leak and completed this
troubleshooting procedure. Return the instrument to normal operation.
• If the flow rate is >100 nL/min, go to the next step.
2. To check the solvent line that connects the mixing Tee outlet to port 4 of valve S, do the
following:
a. On the Home > Overview page, set valves A and W to the following positions to
return the system to atmospheric pressure:
• Valve A: Center
• Valve W: 1–6
For information about changing the valve positions, see
on page 165.
b. Disconnect the nanoViper fitting from the mixing Tee outlet.Then, block the mixing
Tee outlet with a Viper blind plug (see
190.
Figure
"Using the Valve Controls"
156).
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