Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 303

d. Pipette the appropriate solvent into the pump head.
Figure 7.
Adding solvent to the pump head
e. Set flow rate to –300 µL/min and the volume to
140 µL. Then press Start.
The piston moves down, drawing solvent into the
pump head. Make sure the pump head is filled with
solvent during the entire retraction of the piston.
CAUTION Be careful not to spill solvent
onto the printed circuit board behind the
LED panel.
7. Mount the stainless steel Viper back on the pump head
and the pressure sensor. (see
8. Reinstall the right side panel.
9. Reset the usage counter for the pump as follows:
a. Press Maintenance > Devices.
b. Select the pump from the list of devices.
c. Press the Summary tab.
Figure 2 on page 279).
d. Press Reset.
The value in the Intermediate Volume box resets to 0.
10. To draw fresh solvent through the solvent lines and to
remove air, do the following:
a. Open the Parameters page for the Purge Solvent script
as described in
b. In the Purge Iterations box, enter 10.
c. Select the check box for the appropriate pump.
d. Press Start and wait for the system to perform 10
purge cycles.
11. To remove air from the pump head, do the following:
a. In the Name category on the Maintenance > Scripts
page, select Flush Air.
b. Press the Parameters tab.
c. In the Flush Threshold [µL] box, enter 12.
d. Press Start.
e. Wait for the script to finish.
12. Run the Leaks script for the pump with the new piston seal
a. On the Maintenance > Scripts page, select Test in the
Category list and then Leaks in the Name list.
b. Press the Parameters tab.
c. Select the check box for the pump with the new seal.
d. Press Start.
13. If the script detects a leak, replace the stainless steel tubing
that connects the pump to the pressure sensor.
14. When the instrument is leak tight, it is ready for use.
281
step 1 on page 279.