Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 204

7
Troubleshooting
Running a System Leak Test
182 EASY-nLC 1200 Troubleshooting & Maintenance Guide
Figure 145. UHPLC fused-silica union (enlarged cross section)
Microferrule fitting
(IDEX P/N PK-152)
b. Slide a female nut and a microferrule onto the free ends of both the Column Out
tubing and the Waste In tubing.
c. Insert the ferrules into the ends of the PEEK holder and hand tighten the nuts.
Figure 146 shows the solvent lines connected to the UHPLC fused-silica union.
Figure 146. UHPLC fused-silica union connected to the Column Out and Waste In lines
Microferrule fitting
Female nut
3. Open the Test – Leaks script as follows:
a. Press Maintenance > Scripts.
b. In the Category list, select Test.
c. In the Name list, select Leaks.
4. Set the parameters for the system leak test as follows:
a. Press the Parameters tab.
b. In the Value list, select System.
5. Press Start.
The script starts and the Output page opens. During the duration of this script, the
EASY–nLC instrument pressurizes the flow paths highlighted with a dotted red line in
Figure 32 on page 45
identify and localize leaks. When the script ends, the Output page displays a message.
Female nut
PEEK holder
PEEK holder
and monitors the flow sensors and pump piston movements to
Stainless steel cartridge
with internal bore
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