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Summary of Contents for Thermo Scientific EASY-Spray Series
- Page 1 EASY-Spray Series Ion Source User Guide 60053-97275 Revision B June 2018...
- Page 2 Unity is a registered service mark; and Acclaim, Dionex, EASY-Spray, LCQ, LTQ, Optima, Orbitrap, Orbitrap Fusion, Thermo Scientific, Tribrid, UltiMate, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States. Fisher Scientific is a registered trademark of Fisher Scientific Co.
- Page 3 WEEE Directive 2012/19/EU Thermo Fisher Scientific is registered with B2B Compliance (B2Bcompliance.org.uk) in the UK and with the European Recycling Platform (ERP-recycling.org) in all other countries of the European Union and in Norway. If this product is located in Europe and you want to participate in the Thermo Fisher Scientific Business-to-Business (B2B) Recycling Program, send an email request to weee.recycle@thermofisher.com with the following information:...
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Page 5: Table Of Contents
Chapter 2 Installing the EASY-Spray Series Ion Source ......7 Connecting Power to the Ion Source ........7 Powering the Ion Source for an EASY-nLC 1200/MS System . - Page 6 Index ..............47 EASY-Spray Series Ion Source User Guide...
- Page 7 Temperature controller PCB (P/N ES233) ......36 Figure 25. EASY-Spray ion source (bottom) without the temperature controller PCB ..37 Thermo Scientific EASY-Spray Series Ion Source User Guide...
- Page 8 Figures viii EASY-Spray Series Ion Source User Guide Thermo Scientific...
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Page 9: Preface
Preface The EASY-Spray Series Ion Source User Guide describes the hardware components, and provides installation and configuration procedures for the Thermo Scientific™ EASY-Spray™ Series ion sources. Depending on the installed camera type, you view the ion source spray by using the camera software or an LCD monitor that is placed on the mass spectrometer (MS). -
Page 10: Viewing The Product Manuals
To return to the document list, click the browser Back button. Providing Documentation Feedback To suggest changes to the documentation Complete a brief survey about this document by clicking the button below. Thank you in advance for your help. EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 11: Compatible Mass Spectrometers
MSs. For information about your specific instrument, refer to its manuals. CAUTION If you connect the EASY-Spray Series ion source to another type of MS, you might impair the safety protection provided by the equipment. Table 1. Ion sources and compatible mass spectrometers... - Page 12 Risk of eye injury: Eye injury can occur from splattered chemicals, airborne particles, or sharp objects. Wear safety glasses when handling chemicals or servicing the ion source. Trip obstacle: Be aware of cords or other objects located on the floor. EASY-Spray Series Ion Source User Guide Thermo Scientific...
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Page 13: Safety Precautions
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable North American and European regulations. The EASY-Spray Series ion sources were tested as part of the Thermo Scientific MS system, which meets the applicable requirements for electromagnetic compatibility (EMC) and product safety. For additional regulatory information, refer to the MS manuals. -
Page 14: Contacting Us
Note To provide feedback for this document, go to surveymonkey.com/s/PQM6P62 or send an email message to Technical Publications (techpubs-lcms@thermofisher.com). You can use your smartphone to scan a QR Code, which opens your email application or browser. EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 15: Introduction
Key benefits of the EASY-Spray Series are as follows: • Simple and robust design for nano liquid chromatography (nanoLC)/MS integration • Better separation with zero dead volume (ZDV) connections •... -
Page 16: Advantages Of Nanoelectrospray
MS. Note The MS’s instrument control applications use the terms nanospray and NSI. Functional Description This section describes the following principal components of the EASY-Spray Series ion source and their functions: •... -
Page 17: Source Housing
Introduction Functional Description Source Housing The EASY-Spray Series ion source housing (Figure 2 Figure 3) arrives assembled and is easy to install on the appropriate MS (see Table 1). The ion source housing includes two locking levers, two observation windows, a position-adjustable column holder, and a controller printed circuit board (PCB). -
Page 18: Figure 3. Easy-Spray Ion Source (P/N Es081) With The Usb Camera
Introduction Functional Description Figure 3. EASY-Spray ion source (P/N ES081) with the USB camera Camera observation window Locking lever Source housing Front observation window Column holder EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 19: Temperature Controller Pcb
Functional Description Temperature Controller PCB The EASY-Spray Series ion source contains a temperature controller PCB that powers and controls the heat generated for the connected EASY-Spray column. The temperature controller PCB includes an input power socket, an output heater socket, a temperature control knob, and an actual temperature display. -
Page 20: Controller Power Supply
Introduction Functional Description Controller Power Supply The installation method for the EASY-Spray Series ion source includes the controller power supply when using an LC instrument other than the EASY-nLC 1200. For instructions, see Powering the Ion Source for Other nanoLC/MS Systems. -
Page 21: Installing The Easy-Spray Series Ion Source
• Removing the EASY-Spray Series Source Connecting Power to the Ion Source This section describes how to connect power to the EASY-Spray Series ion source when using either the EASY-nLC 1200 instrument or another LC instrument, such as the Thermo Scientific Dionex™... -
Page 22: Powering The Ion Source For An Easy-Nlc 1200/Ms System
Installing the EASY-Spray Series Ion Source Connecting Power to the Ion Source Powering the Ion Source for an EASY-nLC 1200/MS System Instead of using the controller power supply (Figure 5), use the EASY-Spray P-Bus Temp Control Cable (P/N 70005-63055) that comes with the EASY-nLC 1200 instrument to connect the instrument to the ion source. -
Page 23: Powering The Ion Source For Other Nanolc/Ms Systems
CAUTION After completing the power supply connections, route the output cord so that it is not a trip hazard. Attaching the Ion Source Follow these procedures to install the EASY-Spray Series ion source. • To install the ion source onto the MS •... - Page 24 Installing the EASY-Spray Series Ion Source Attaching the Ion Source CAUTION Before you touch an ion source that was recently in use or touch its components, allow the system to cool for a minimum of 20 minutes. CAUTION • Make sure that you do not accidentally lift the release lever located at the top of the API source interface, which could interfere with or prevent installation of the ion source.
- Page 25 Installing the EASY-Spray Series Ion Source Attaching the Ion Source To install the optional Tee holder under the column holder Bend the top part of the Tee holder down to the extended position. Move the sliding lock toward the column holder.
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Page 26: Adjusting The Emitter Tip Position
Installing the EASY-Spray Series Ion Source Adjusting the Emitter Tip Position Adjusting the Emitter Tip Position Before installing the EASY-Spray column, use the emitter positioning tool (Figure 7) and a 3 mm hex key to adjust the position of the column holder. - Page 27 Installing the EASY-Spray Series Ion Source Adjusting the Emitter Tip Position To adjust the emitter tip position Tip To enhance your view of the emitter positioning tool’s tip, install the camera and LCD monitor (if applicable) before you begin this procedure. For instructions, see Chapter 3, “Installing the Camera and LCD Monitor.”...
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Page 28: Removing The Easy-Spray Series Source
The MS automatically switches to off mode after a few seconds. You can store the source in its original shipping box after you remove the camera. The EASY-Spray Series ion source does not require cleaning. EASY-Spray Series Ion Source User Guide... -
Page 29: Chapter 3 Installing The Camera And Lcd Monitor
Dino-Lite™, USB ES218 To install the USB camera Dino-Lite, analog ES216 To install the analog camera and LCD monitor Might require a 1.5 mm hex key to complete. LCD monitor (P/N ES217) Thermo Scientific EASY-Spray Series Ion Source User Guide... -
Page 30: Installing The Usb Camera
4. Plug the USB cord into the data system computer’s USB port. IMPORTANT Do not connect the camera to a USB hub. Tip For troubleshooting purposes, record the USB port used for the camera. EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 31: Installing The Analog Camera And Lcd Monitor
Connect the yellow video connector to the monitor’s Video IN port (see step 1a). d. Connect the black connector to its power supply, and then connect the power supply to an electrical outlet. Thermo Scientific EASY-Spray Series Ion Source User Guide... -
Page 32: Adjusting The Video Picture
If the picture is out of focus, adjust the camera’s focusing wheel. iv. If there is no picture, press the SOURCE button. The monitor displays the selected video input channel (AV1 or AV2) for a few seconds. EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 33: Connecting The Easy-Spray Column Plumbing
LC plumbing and EASY-Spray column connections. Not all parts are required. Note The kit for the EASY-Spray Series ion source supplies the venting Tee, fittings, and sleeves only. You are responsible for supplying the remaining parts and tools. -
Page 34: Installing The Easy-Spray Column For The First Time
EASY-Spray column unusable. Figure 11. EASY-Spray column nanoViper fitting for the LC connection Emitter protective cover Protective cap Heater cable and plug EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 35: Setting The Column Temperature
• (EASY-nLC 1200 instrument) Use either the Thermo Xcalibur Instrument Setup window, or use the EASY-nLC 1200 instrument’s touch screen as follows: On the touch-screen’s Home page (top, right corner), press the EASY-Spray icon, Thermo Scientific EASY-Spray Series Ion Source User Guide... -
Page 36: Using The Nanoviper Fitting
Tip Refer to the packing for your nanoViper fittings to determine compatibility with either the 1000 bar capillaries (beige tubing) or the 1200 bar capillaries (blue tubing). Figure 12. nanoViper fitting PEEK sealing surface Removable knurled tightening tool Screw EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 37: Examples Of Lc Connections To The Easy-Spray Column
CAUTION Wear protective gloves and eye wear when handling the solvent lines. Follow the appropriate topic: • Connecting the EASY-nLC 1200 to the EASY-Spray Column • Configuring the EASY-nLC 1200 Plumbing for Two Columns • Connecting an RSLCnano System to the EASY-Spray Column Thermo Scientific EASY-Spray Series Ion Source User Guide... -
Page 38: Connecting The Easy-Nlc 1200 To The Easy-Spray Column
2. On the Waste In line, insert the fitting into the venting Tee’s bottom port, tighten the fitting by hand until you feel resistance, and then tighten the fitting by an additional 1/8- to 1/4-turn. Do not tighten the fitting by more than a 1/4-turn. EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 39: Figure 14. Tee Holder Secured To The Bottom Of The Column Holder
5. Adjust the Tee holder’s position so that it supports the nanoViper fitting, and then use a 3 mm hex key to tighten the hex bolt on the bottom of the column holder. Thermo Scientific EASY-Spray Series Ion Source User Guide... -
Page 40: Figure 16. Example Of A Two-Column Setup With Nanoviper Fittings And A Viper Union
5. Adjust the Tee holder’s position so that it supports the nanoViper fitting, and then use a 3 mm hex key to tighten the hex bolt on the bottom of the column holder. EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 41: Configuring The Easy-Nlc 1200 Plumbing For Two Columns
Follow the appropriate procedure for a one- or two-column setup for an RSLCnano system. The two-column setup includes a precolumn connected across the autosampler valve. Note To connect the RSLCnano system to the EASY-Spray column, you must use the Thermo Scientific Dionex UltiMate 3000 RSLCnano EASY-Spray Application Kit (P/N 6720.0395). •... -
Page 42: Figure 18. Example Of A One-Column Setup For An Rslcnano System (Direct Injection)
(Figure 18). Figure 18. Example of a one-column setup for an RSLCnano system (direct injection) EASY-Spray column 20 μm ID × 65 cm Viper union Autosampler 20 μm ID 1 μL loop EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 43: Figure 19. Example Of A Two-Column Setup For An Rslcnano System (Preconcentration)
4. Connect the union to the EASY-Spray column to complete the two-column setup (Figure 19). Figure 19. Example of a two-column setup for an RSLCnano system (preconcentration) EASY-Spray column Viper union 20 μm ID Column compartment with two switching 20 μm ID valves Precolumn Thermo Scientific EASY-Spray Series Ion Source User Guide... -
Page 44: Additional Resources
Refer to the Thermo Scientific EASY-nLC Series documentation for the following: • Setting up the column assembly • Modifying the LC instrument before connecting it to the EASY-Spray Series ion source For other LC instruments, refer to their documentation. EASY-Spray Series Ion Source User Guide... -
Page 45: Configuring The Mass Spectrometer For Nsi Mode
Configuring the Mass Spectrometer for NSI Mode Follow the applicable procedures to configure the Thermo Scientific MS for NSI mode. Contents • Configuring the EASY-Spray NG Source’s NSI Parameters • Configuring the EASY-Spray Source’s NSI Parameters Configuring the EASY-Spray NG Source’s NSI Parameters After you complete the instrument configuration, use the Thermo Tune application to configure the NSI source parameters. -
Page 46: Configuring The Easy-Spray Source's Nsi Parameters
The recommended range for the spray voltage is 1.40–2.40 kV. Figure 21. NSI Source dialog box Enter the absolute value. 3. Click OK. EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 47: Maintenance
Maintenance This chapter provides maintenance guidelines, a list of required tools and supplies, and instructions for replacing the temperature controller PCB in the EASY-Spray Series ion source. Contents • Guidelines • Tools and Supplies • Cleaning the EASY-Spray Column Emitter Tip •... -
Page 48: Tools And Supplies
Maintenance Tools and Supplies Tools and Supplies The EASY-Spray Series ion source requires very few tools to perform routine maintenance procedures. Table 6 lists the necessary tools and supplies for maintaining the ion source and column. CAUTION Avoid exposure to potentially harmful materials. -
Page 49: Cleaning The Easy-Spray Column Emitter Tip
Hold onto these components and run the sonicator for 1 minute. This completes the cleaning procedure. If you have further issues with the emitter, you might need to replace the column. Thermo Scientific EASY-Spray Series Ion Source User Guide... -
Page 50: Replacing The Temperature Controller Pcb
IMPORTANT If you remove the temperature controller PCB too quickly or at an angle, you might damage the PCB components or the internal heater cable routed above the PCB. Figure 24. Temperature controller PCB (P/N ES233) EASY-Spray Series Ion Source User Guide Thermo Scientific... -
Page 51: Figure 25. Easy-Spray Ion Source (Bottom) Without The Temperature Controller Pcb
24). 7. Using a 2.5 mm hex key, install the four hex screws into the bottom of the ion source. You can now install the EASY-Spray Series ion source back onto the MS. To dispose of the old PCB, see WEEE Directive 2012/19/EU. - Page 52 Maintenance Replacing the Temperature Controller PCB EASY-Spray Series Ion Source User Guide Thermo Scientific...
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Page 53: Chapter 7 Troubleshooting
You can resolve most problems by using a new EASY-Spray column. If you need further troubleshooting assistance, contact your local Thermo Fisher Scientific field service engineer. See Contacting Table 7. EASY-Spray Series ion source problems, causes, and possible solutions (Sheet 1 of 3) Problem Cause Solution Camera issues The light is not on. - Page 54 Troubleshooting Table 7. EASY-Spray Series ion source problems, causes, and possible solutions (Sheet 2 of 3) Problem Cause Solution Signal issues The signal The parameters for the LC, MS, Try these solutions: intensity is weak. or both might need adjusting.
- Page 55 Troubleshooting Table 7. EASY-Spray Series ion source problems, causes, and possible solutions (Sheet 3 of 3) Problem Cause Solution (continued) The source high-voltage (HV) Check the high voltage contact on the back of the ion connection might be unstable. source (page 10) and the front of the mass spectrometer.
- Page 56 Troubleshooting EASY-Spray Series Ion Source User Guide Thermo Scientific...
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Page 57: Chapter 8 Replaceable Parts
Replaceable Parts To order any of these parts for the EASY-Spray Series ion sources, contact your local Thermo Fisher Scientific service engineer. Spare Parts A/B mixing/venting Tee ..........SC901 Camera, analog (composite video) interface, and power supply . - Page 58 EASY-Spray transfer line, 7 μm ID emitter, 20 μm × 50 cm ....ES791 EASY-Spray transfer line, microflow, 20 μm ID emitter, 75 μm × 50 cm ..ES792 EASY-Spray Series Ion Source User Guide Thermo Scientific...
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Page 59: Chapter A Contents Of The Installation Kits
Chapter 8, “Replaceable Parts.” Note As of 2018, these ion source kits exclude the LCD monitor (P/N ES217) and the 1.5 mm hex key. Table 8. Contents of the EASY-Spray Series ion source kits (Sheet 1 of 2) Image Description... - Page 60 Contents of the Installation Kits Table 8. Contents of the EASY-Spray Series ion source kits (Sheet 2 of 2) Image Description Quantity Part number Tools: 1 set ES232 • Emitter positioning tool • 3 mm hex key A/B mixing/venting Tee, stainless steel,...
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Page 61: Index
EASY-Spray P-Bus Temp Control Cable electrical wall outlets, number of maintenance emitter guidelines positioning tool tools, equipment, and chemicals See also procedures mass spectrometer diagnostics NSI mode, configuring preparing for the source figures, list of Thermo Scientific EASY-Spray Series Ion Source User Guide... - Page 62 See ion source spray troubleshooting voltage readback value setting the value, EASY-Spray setting the value, EASY-Spray NG symbols, meaning EASY-Spray Series Ion Source User Guide Thermo Scientific...
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