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7
Troubleshooting
Troubleshooting a System Blockage
Troubleshooting a System Blockage when Test Solvent A Fails
210
EASY-nLC 1200 Troubleshooting & Maintenance Guide
Follow this procedure when the Back Pressure test for Test Solvent A fails (see
Back Pressure Test for the A and B Solvent Paths"
❖ To verify the system blockage
1. Run the Precolumn Equilibration script and check the backpressure as follows:
a. On the Maintenance > Scripts page, select Prepare in the Category list and then
Precolumn Equil in the Name list.
b. Press the Parameters tab.
c. In the Volume [µL] box, enter 140 µL.
d. In the Flow [µL/min] box, enter 2 µL/min.
e. Leave the Max Pressure [bar] box blank.
f.
Press Start.
g. Monitor the pump A pressure trace.
2. Depending on the backpressure, do the following:
• If the backpressure is below the pressure threshold, the system blockage has been
removed. To confirm that the backpressure is below the pressure threshold, rerun the
Back Pressure script with the Test Solvent A check box selected.
• If the backpressure is above the pressure threshold, keep the Precolumn Equil script
running and go to
fails"
on
page
213.
Instrument
EASY-nLC 1200
on
"To locate the source of the blockage when the Test Solvent A test
Pressure threshold
Solvent A: 175 bar / Solvent B: 250 bar
page
209).
"Running the
Thermo Scientific
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Troubleshooting
