Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 261

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4. Center the needle above the vial or well as follows:
• Press the Needle In/Out (3) buttons to adjust the x-axis position.
• Press the Needle Left/Right (4) buttons to adjust the y-axis position.
y axis
x axis
5. Using the step buttons in the Needle Up/Down area (5), adjust the needle height as
follows:
a. Use the double step button until the needle reaches the bottom - indicated as the
O-ring on the needle moves away from the PEEK nut.
b. Use 2 double steps to lift the needle up.
Tip For the ASC autosampler, the O-ring at the top of the needle holder rises a
little when the needle reaches the bottom of the vial or well (see
Figure 176. O-ring pushed slightly above the ASC needle holder
c. Use single steps to move needle down and accurately reach the bottom.
d. Use 2 single steps to move needle just off the bottom.
The appropriate needle height is two small step higher than the bottom of the vial
or well. If a very small sample volume is used and everything needs to be aspirated,
the appropriate needle height is one small step higher than the bottom of the vial or
well.
1
In
A
Right
Left
EASY-nLC 1200 Troubleshooting & Maintenance Guide
8
Calibrating the Autosampler's XYZ Robot
Calibrating Plates
Out
Figure
Observe when the O-ring moves up a
bit, or gently hold needle tubing as you
press the step button.
176).
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