Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 306

Figure 4. Autosampler needle connected to the z-axis needle holder
ASC z-axis needle holder
Installing a New Autosampler Needle
❖ To install an new autosampler needle
1. Mount the new autosampler needle in the z-axis holder. Check
that the small spring is situated between the PEEK nut and the
plastic stop.
2.
a. Guide the end of the needle that connects to Valve S through
the large hole in the metal plate that separates the tray
compartment from the solvent system compartment.
b. Install the black needle holder in the side plate.
3. Connect the needle to port 1 of valve S as follows:
a. Insert the nanoViper fitting into the receiving port. Then
turn the screw clockwise until you feel resistance.
b. Turn the screw clockwise to an angle between 0 and
45 degrees (1/8-turn) using the black knurled nut.
4. Recalibrate the needle position.
5. Press Maintenance > Scripts.
6. In the Category list, select Prepare.
7. In the Name list, select Purge Solvent. Then run two iterations of
the Purge Solvent script for Pump S.
8. In the Name list, select Flush Air. Then run the Flush Air script
for Pump S with a flush volume threshold of 12 µL.
284