Calibrating Plates - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

8
Calibrating the Autosampler's XYZ Robot

Calibrating Plates

Calibrating Plates
236 EASY-nLC 1200 Troubleshooting & Maintenance Guide
4. Install the wash bottle in position W4.
IMPORTANT For the autosampler to determine the appropriate depth for the needle,
the wash bottle in position W4 must contain the needle wash insert.
5. Remove the left side panel so that you can watch the movement of the needle.
To calibrate the wash bottle and extra vial positions, go to
Extra Vial Positions" on
For information about selecting or creating a plate format, see
page 231.
IMPORTANT When calibrating the XYZ robot for vial or microwell plates, make sure to
remove the vial caps or plate covers. Keeping the caps or covers on, makes the needle
susceptible to bending and breaking.
During a sample run, the z-axis needle holder moves to the specified sample position along
the xy-plane. When the z-axis needle holder reaches the specified location, the needle descends
along the z-axis into the sample vial or well.
Calibrating plates requires determining the xyz positions for the top left and bottom right vial
or well. Table 29 lists the top left and bottom right positions for the three plate formats.
Table 29. Top left and bottom right positions on the plate formats
Plate format
6 × 8
8 × 12
16 × 24
page 241.
Top left position
A1
A1
A1
"Calibrating the Wash Bottle and
"Managing Plate Formats"
Bottom right position
F8
H12
P24
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on