Specifications; Performance Specifications - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

Specifications

Performance Specifications

Thermo Scientific
These sections describe the performance and technical specifications for the EASY-nLC
syringe pumps and autosampler:
• Performance Specifications
• Technical Specifications
Table 3 lists the performance specifications for the EASY-nLC 1200 instrument.
Table 3. Performance specification (Sheet 1 of 2)
Item
Flow range (gradient)
Flow while loading and
equilibrating
Pressure range
Retention time
reproducibility (RT RSD)
Peak widths
Carryover
Autosampler sample pickup
volume range
Injection reproducibility
(injection RSD)
Injection linearity
Specification
20 to 2000 nL/min
Recommended: 100 to 1000 nL/min
Up to 25 µL/min
0 to 1200 bar
Within the recommended flow range: 0.1 to 0.4%
Outside the recommended flow range: typically better
than 1%
Typically 3 to 5 seconds full width at half maximum (fwhm),
using PepMap™ nano-bore columns as supplied at delivery
Typically < 0,05% with Caffeine; < 0,5% with 100 fmol
tryptic digest of BSA
Conditions: Injection of 100 fmol tryptic digests of BSA on
Pepmap columns as supplied at delivery and by using the
standard wash procedure for the autosampler
0.1 to 18 µL with the standard 20 µL loop
0.1 to 48 µL with a 50 µL loop
0.2% at 5 µL pickup
3.0% at 0.1 µL pickup
0.9985 at 0.5 to 10 µL injection volume
EASY-nLC 1200 Troubleshooting & Maintenance Guide
1
Introduction
Specifications
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