Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual page 62

3
Maintenance Scripts and Service Records
Maintenance Scripts
40 EASY-nLC 1200 Troubleshooting & Maintenance Guide
Figure 27 shows the contact closure cable for an EASY-nLC instrument that connects to a
Thermo Scientific mass spectrometer.
Figure 27. Thermo Fisher contact closure cable, part number LC160 (part name)
Tip Use the Thermo Fisher contact closure cable (LC160) to make contact closure
between the EASY-nLC instrument and a Thermo Scientific mass spectrometer.
Connect the 12-pin connector to the back panel of the EASY-nLC instrument. For an
LTQ™ or TSQ™ mass spectrometer, connect the two-pin connector to the mass
spectrometer's Start In pins. For a TSQ Quantiva™, a TSQ Endura™, or an Orbitrap
Fusion™ mass spectrometer, modify the contact closure cable as described in the
EASY-nLC 1200 Getting Started Guide.
2. Check the contact closure settings for the LC/MS system as follows:
a. On the EASY-nLC touch screen, press Configuration > Connections.
b. For a Thermo Scientific mass spectrometer, make the following selections:
• In the Instrument (cable no.) list, select either Thermo Scientific (LC160) or
Thermo Scientific Orbitrap Fusion (LC160).
• In the Protocol list under Contact Closure Settings, select One-way.
• In the State at Start list under Contact Closure Settings, select Open.
3. Turn on the mass spectrometer and set it up to wait for a contact closure signal.
• For a third-party mass spectrometer, follow the instructions in the documentation
provided with the mass spectrometer. Then, go to
• For an LTQ Series mass spectrometer, go to
4. For an LTQ Series mass spectrometer, set up the mass spectrometer to wait for a contact
closure signal as follows:
a. Open the Tune Plus window.
b. On the menu bar, choose Control > Acquire Data.
Figure 28 shows the Control menu of the Tune Plus window for an LTQ Series mass
spectrometer.
step 5.
step 4.
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