Isocratic Flow - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

3
Maintenance Scripts and Service Records
Maintenance Scripts

Isocratic Flow

38 EASY-nLC 1200 Troubleshooting & Maintenance Guide
The Isocratic Flow script runs solvents A and B at a user-defined mixture ratio. The script uses
the AFC system to accurately control the flow. For details on the AFC system control, refer to
the EASY-nLC 1200 Getting Started Guide. Use this script to tune the mass spectrometer at a
given B percentage or when cleaning the instrument, the columns, or both.
the parameters for the Isocratic Flow script.
Figure 25. Isocratic Flow script parameters
❖ To run the Isocratic Flow script
1. Select the Isocratic Flow script as follows:
a. Press Maintenance > Scripts.
b. In the Category list, select Prepare.
c. In the Name list, select Isocratic Flow.
2. Set up the parameters (see
a. Press the Parameters tab.
b. Enter the appropriate values:
• In the Volume box [µL], enter the total volume to be delivered by the pumps.
Note If you select the Run Indefinitely check box, the script ignores this
volume setting.
• In the Flow box [µL/min], enter the flow rate to be used.
Figure 25) for this script as follows:
Figure 25 shows
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