Precolumn Equilibration - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

3
Maintenance Scripts and Service Records
Maintenance Scripts

Precolumn Equilibration

34 EASY-nLC 1200 Troubleshooting & Maintenance Guide
❖ To run the Flush Air script
1. Open the Flush Air script as follows:
a. Press Maintenance > Scripts.
b. In the Category list, select Prepare.
c. In the Name list, select Flush Air.
2. Set up the parameters for this script as follows:
a. Press the Parameters tab.
b. In the Flush Threshold [µL] box, enter 12.
3. Press Start.
The script ends after the first iteration that gives a flush volume below the set threshold.
The Precolumn Equilibration script equilibrates the precolumn with solvent from pump A.
Use this script to equilibrate or flush the precolumn and to determine a suitable flow rate for
the sample loading step in your method.
Figure 23 shows the parameters for the Precolumn Equilibration script.
Figure 23. Precolumn Equilibration script parameters
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