Autosampler Problems - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

Autosampler Problems

Table 11. Autosampler troubleshooting tips
Symptom
The needle cannot
penetrate the plastic
film on the microtiter
plate.
XYZ robot error
A server warning
message about the plate
format for the
autosampler
configuration pops up
when you submit a
batch.
Thermo Scientific
Table 11 lists troubleshooting tips for the autosampler.
Note For information about troubleshooting sample pickup, see
Autosampler Aspiration"
Possible causes
The needle tip is damaged or
bent.
Something is blocking the
movement of the autosampler's
XYZ arm and causing a step loss
on one of the motors.
The error repeats without any
visible reason.
The plate format has been
deleted from the system
configuration. For information
about deleting a plate format,
refer to the EASY-nLC 1200
Getting Started Guide.
on page 220.
Action
Do the following:
1. Check the configuration settings for the adapter tray
on the Tools page for the autosampler device, which
you can access from the Maintenance > Devices page.
2. Eject the tray.
3. Remove the left side panel and visually inspect the
needle tip.
4. Do one of the following:
• If the needle tip is bent or damaged, replace it
(see "Replacing the Autosampler Needle"
page 85).
• If there is any residue on the needle tip, wipe the
needle tip with a lint-free tissue soaked in
LC/MS-grade ethanol.
Remove the obstacle and try again. You might need to
calibrate the autosampler for more precise penetration of
the microtiter plate or the wash bottles.
For information about calibrating the autosampler, see
Chapter 8, "Calibrating the Autosampler's XYZ Robot."
Contact Thermo Fisher Scientific:
• us.customer-support.analyze@thermofisher.com
• eu.techsupport.cms@thermofisher.com
If you want to run the batch, you must create the plate
format again.
For information about selecting, creating, and deleting
plate formats, see
EASY-nLC 1200 Troubleshooting & Maintenance Guide
7
"Troubleshooting the
"Managing Plate Formats"
Troubleshooting
Troubleshooting Tips
on
on page 231.
131