Direct Infusion - Thermo Scientific EASY-nLC 1200 Troubleshooting And Maintenance Manual

Direct Infusion

Thermo Scientific
Use the Direct Infusion script to analyze samples by direct infusion or to optimize the spray
stability by infusing a known peptide.
In a direct infusion experiment, sample is introduced into the mass spectrometer without
chromatographic separation by an analytical column. The EASY-nLC instrument picks up the
sample volume from the specified location, and then pumps it directly into the ion source
using the solvent flow from pump A.
Figure 40 shows the parameters for the Direct Infusion script.
Figure 40. Direct Infusion script
❖ To set up the system for a direct infusion experiment
1. Connect an emitter to the Column Out tubing.
Figure 41 shows an example of a direct infusion setup for an EASY-nLC 1200
instrument.
Figure 41. Setup for a direct infusion experiment
Column Out line
2. Mount the assembly onto your nanoflow ion source.
3
1/32 in. fitting
PEEK adapter union for 1/16 in. OD and 1/32 in. OD tubing
1/16 in. fitting
EASY-nLC 1200 Troubleshooting & Maintenance Guide
Maintenance Scripts and Service Records
Maintenance Scripts
Emitter ES 542 and sleeve
55