Thermo Scientific Evolution 200 Series User Manual
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Thermo Scientific Evolution 200 Series User Manual

Thermo Scientific Evolution 200 Series User Manual

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Table of Contents

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UV-Vis Instruments
Evolution 200 Series
User Guide
269-251400 Revision A
August 2010

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Summary of Contents for Thermo Scientific Evolution 200 Series

  • Page 1 UV-Vis Instruments Evolution 200 Series User Guide 269-251400 Revision A August 2010...
  • Page 2 © 2010 Thermo Fisher Scientific Inc. All rights reserved. Microsoft, Windows, Excel and Vista are either trademarks or registered trademarks of Microsoft Corporation in the United States and/or other countries. DYMO is either a trademark or registered trademark of Sanford L.P.

  • Page 3: Table Of Contents

    Thermo Software IQ ..........24 Thermo Scientific Evolution 200 Series User Guide...
  • Page 4 System Tab ..........101 Evolution 200 Series User Guide...
  • Page 5 Chapter 3 Evolution 200 Series Spectrophotometers .......103 Safety Considerations ......... . . 103 Safety Label Locations .
  • Page 6 Making Protein A280 Measurements ......167 Evolution 200 Series User Guide Thermo Scientific...
  • Page 7 Bradford Standard Curves ........197 Making Protein Bradford Assay Measurements ..... . 197 Thermo Scientific Evolution 200 Series User Guide...
  • Page 8 60 Degree Specular Reflectance ........247 viii Evolution 200 Series User Guide Thermo Scientific...
  • Page 9 Installation and Operation........275 Thermo Scientific Evolution 200 Series User Guide...
  • Page 10 Other System Failures ........310 Evolution 200 Series User Guide...
  • Page 11 Variable Pathlength Cell Holder Slide ......351 Thermo Scientific Evolution 200 Series User Guide...
  • Page 12 Troubleshooting ..........394 Evolution 200 Series User Guide...
  • Page 13 Troubleshooting ..........428 Thermo Scientific Evolution 200 Series User Guide xiii...
  • Page 14 Assigning Signature Meanings ........463 Evolution 200 Series User Guide...

  • Page 15: Introduction

    Introduction This document covers INSIGHT software and the Evolution 200 series UV-Visible spectrophotometers and accessories. Contents • About the Software and Hardware • Contacting Us • Product Warranty • Trademarks • Site Preparation and Safety About the Software and Hardware Contents •...

  • Page 16: Setting Up The Instrument

    Description Introduction Overview of the instrument and this document plus information about registering your instrument, contacting us, and the system warranty. Evolution 200 Series Complete instructions for using and maintaining the Spectrophotometers instrument. Evolution 200 Series User Guide Thermo Scientific...

  • Page 17: Contacting Us

    WARNING Indicates a hazardous situation which, if not avoided, could result in death or serious injury. Contacting Us For Technical Support, please contact: Thermo Fisher Scientific 5225 Verona Road Madison, WI 53711-4495 U.S.A. Telephone: 1 800 532 4752 E-mail: us.techsupport.analyze@thermofisher.com World Wide Web: http://www.thermo.com/spectroscopy Thermo Scientific Evolution 200 Series User Guide...

  • Page 18: E-Mailing Data Files

    Thermo Fisher Scientific’s control. Related Topics Warranty Period Items Not Covered by Warranty Warranty Repair Shipping Cost for Items Covered by Warranty Update Policy Evolution 200 Series User Guide Thermo Scientific...

  • Page 19: Warranty Period

    In the event that on-site warranty service is required, and no on-site service contract is active on this product, the customer is responsible for any travel expenses, labor costs, and additional expenses which may be incurred in the performance of on-site warranty service. Thermo Scientific Evolution 200 Series User Guide...

  • Page 20: Shipping Cost For Items Covered By Warranty

    Microsoft, Windows, Excel and Vista are either trademarks or registered trademarks of Microsoft Corporation in the United States and/or other countries. DYMO is either a trademark or registered trademark of Sanford L.P. in the United States and/or other countries. Evolution 200 Series User Guide Thermo Scientific...

  • Page 21: Site Preparation And Safety

    Site Preparation and Safety Before using the system, read the site preparation and safety manual on the provided documentation CD. Always follow the safety precautions in that manual and in this document when using the system. Thermo Scientific Evolution 200 Series User Guide...
  • Page 22 Introduction Site Preparation and Safety Evolution 200 Series User Guide Thermo Scientific...

  • Page 23: Insight Software

    Fixed The Fixed application measures the light passing through the sample at one or more wavelengths. To work with this application, click Fixed in the right pane in Home. Thermo Scientific Evolution 200 Series User Guide...

  • Page 24: Scan

    Quant Performing a Quantitative Analysis Rate Use the Rate application to make time-based kinetics measurements. To work with this application, click Rate in the right pane in Home. Related Topics The INSIGHT Window Evolution 200 Series User Guide Thermo Scientific...

  • Page 25: The Insight Window

    Alternatively, click this icon on the Windows taskbar. If the taskbar is not visible, click the bottom (or other) edge of the Windows desktop to display it. To “right-click” a software feature on a Local Control system 1. Click the feature. 2. Display the keyboard by clicking Thermo Scientific Evolution 200 Series User Guide...

  • Page 26: Navigation Pane

    The main task buttons (present when the software starts) provide access to basic operations: Button Description Home Gives access to user groups and their applications. (The default is Classic.) My Data Provides tools for storing and retrieving data and other information. Evolution 200 Series User Guide Thermo Scientific...
  • Page 27 Available only after a blank measurement for some applications. May measure single or multiple samples, depending on the application settings. Measures the baseline for the application. Thermo Scientific Evolution 200 Series User Guide...

  • Page 28: Right Pane

    ID. The last sample measured appears in red at the top of the legend. Related Topics Task Buttons Action Buttons Right Pane The right pane in Home includes: Evolution 200 Series User Guide Thermo Scientific...

  • Page 29: Data Display

    Setting Application Parameters Measuring Samples Menus Data Display For some applications, clicking the Measure task button provides a data display in the right pane for viewing sample data immediately after acquisition previously acquired data. Thermo Scientific Evolution 200 Series User Guide...
  • Page 30 (or to be measured). The table below shows the results of analysis operations and whether sample points fall within specified control limits. Right-click the display to access these features (if available for the current data type): Evolution 200 Series User Guide Thermo Scientific...
  • Page 31 Sample Legend Specifies whether to display sample IDs near the upper-left corner of the pane. Line Type Specifies whether to display scan spectra using solid lines or individual data points. Thermo Scientific Evolution 200 Series User Guide...
  • Page 32 Tool Value displays the measurement made by the Peak/valley measurement tool or peak area tool as a label attached to the spectrum by a line. Evolution 200 Series User Guide Thermo Scientific...
  • Page 33 Related Topics Working With Labels Measuring Samples Right Pane Measurement Results The INSIGHT Window Working With Labels Some operations place labels on the displayed data. Here is an example: Thermo Scientific Evolution 200 Series User Guide...
  • Page 34 To delete a label Right-click it and choose Delete. Related Topics Using the Palette Tools and View Finder Finding Peaks in Scan Data Finding Value Level Crossings in Scan Data Evolution 200 Series User Guide Thermo Scientific...

  • Page 35: Menus

    Prints the current report to the default printer. Use Current Settings Makes the current workbook settings and report configuration as Default selections the default choices for a newly opened workbook for the application. Thermo Scientific Evolution 200 Series User Guide...
  • Page 36 Saves Measure Rate results in a file with the extension .xml. If opened in Excel®, the data is displayed with one column per spectrum, and one sample per sheet. The Help menu includes: Evolution 200 Series User Guide Thermo Scientific...

  • Page 37: Instrument Status Monitors

    If a system problem occurs, a red X may appear on the icon. Click it to see information about the problem. Related Topics Accessories Accessories Tab for Fixed Accessories Tab for Scan Accessories Tab for Quant Accessories Tab for Rate Thermo Scientific Evolution 200 Series User Guide...

  • Page 38: Thermo Software Iq

    Clicking the button displays action buttons for starting sample measurement or other data acquisition. The provided buttons and their availability depend on the current application and whether necessary operations have been performed. See Action Buttons. Evolution 200 Series User Guide Thermo Scientific...

  • Page 39: Performing A Quantitative Analysis

    Settings. To access features for quantifying sample data, click Measure Quant. To specify the predefined formulas, units and advanced equations to be available, click Options and use the Formulas & Units tab. Thermo Scientific Evolution 200 Series User Guide...

  • Page 40: Quantifying Sample Data

    Note To start an analysis immediately after viewing or changing the template settings, click the Measure action button instead and skip to step 3. 2. Click Measure. 3. Follow the instructions that appear. 4. When samples information appears, modify it if desired. More: Evolution 200 Series User Guide Thermo Scientific...
  • Page 41 To measure a sample again, right-click its row in the results table and choose Remeasure. After the remeasurement, the previous information for the sample is crossed out (but not removed from the table). Related Topics Quantifying Samples Using Standards Performing a Quantitative Analysis Data Display Thermo Scientific Evolution 200 Series User Guide...
  • Page 42 To remove a standard, select it, right-click the table and choose Clear Standard. To remove all the standards, choose Clear Table. To save standards information for later use, use Export Standards. 5. Click Continue. 6. Follow the instructions that appear, installing the specified standards. Evolution 200 Series User Guide Thermo Scientific...
  • Page 43 If Sample averaging was set to Triplicate, “D” and “T” at the end of sample names indicate the second and third measurements to be made, respectively. Thermo Scientific Evolution 200 Series User Guide...
  • Page 44 (If there are two analysis wavelengths, the concentration value is the average of the values for those wavelengths.) If Use concentration limits was selected on the Samples tab, horizontal limit lines show whether the concentrations are within the specified limits: Evolution 200 Series User Guide Thermo Scientific...

  • Page 45: Performing A Kinetics Measurement

    Click Rate in the right pane in Home to set up and perform a kinetics measurement. The tasks buttons below become available. set Rate parameters, click Settings. To access features for performing the measurement, click Measure Rate. Thermo Scientific Evolution 200 Series User Guide...

  • Page 46: Performing Kinetics Measurements

    If necessary, the Zero action button can be used to remeasure the zero sample. 6. When samples information appears, modify it if desired. To enter previously saved samples information, use Load Samples. To save the samples information, use Save Samples. Evolution 200 Series User Guide Thermo Scientific...
  • Page 47 To delete a row from the table, right-click it and choose Delete Selected Row. To delete all the information from the table, right-click it and choose Clear Table. b. Click Update to generate kinetic rate models, each of which appears as a line across the data. Thermo Scientific Evolution 200 Series User Guide...
  • Page 48 To perform a scan kinetics measurement 1. Make sure Scan data acquisition is selected on the Type tab in Settings in Rate. 2. Click Measure Rate. 3. Click Zero. Evolution 200 Series User Guide Thermo Scientific...
  • Page 49 A scroll bar is provided if spectra are out of view. Right-click the Data tab to access features for changing the scale or adding annotation. Data Display for more information. A result table appears below the spectra. Thermo Scientific Evolution 200 Series User Guide...

  • Page 50: Using The Palette Tools And View Finder

    Using the Region-Threshold Tool Using the View Finder Using the Spectral Cursor Tool Use the spectral cursor tool (if present) to view the X and Y values of a point in a spectrum. Evolution 200 Series User Guide Thermo Scientific...

  • Page 51: Using The Peak/Valley Measurement Tool

    3. Drag the line by its diamond-shaped handle to the peak or valley to measure. 4. Drag the triangular baseline handles to the desired baseline endpoints. The height or depth of the peak or valley appears below the palette. Thermo Scientific Evolution 200 Series User Guide...
  • Page 52 The X values of the endpoints appear in the Baseline readout. The defined area is shaded, and its measurement appears in the Corrected area readout. Here is an example showing the region limits and baseline endpoints adjusted to measure the corrected area of a peak: Evolution 200 Series User Guide Thermo Scientific...
  • Page 53 Note To display the palette, click Drag the vertical lines in the data display or drag horizontally between them to move both at once. Related Topics Using the Selection Tool Using the Spectral Cursor Tool Thermo Scientific Evolution 200 Series User Guide...
  • Page 54 To display a different region of the same size There are three ways to do this: • To move the spectrum to the right, click the top half of the Roll button. Evolution 200 Series User Guide Thermo Scientific...

  • Page 55: Setting Application Parameters

    Action buttons for the current application may include: Button Description Displays data acquisition features for the application and starts an acquisition. Thermo Scientific Evolution 200 Series User Guide...

  • Page 56: Fixed

    A larger spectral bandwidth increases the amount of energy that passes through the sample. Increasing the bandwidth can improve the signal-to-noise ratio; however, a larger spectral bandwidth decreases the ability to resolve closely spaced spectral features. Evolution 200 Series User Guide Thermo Scientific...
  • Page 57 (and listed in the table to the right of the display), indicate whether each measured data point is within specification. Enter the desired upper and lower limits for each wavelength in the table, using the appropriate units for the selected data mode. Thermo Scientific Evolution 200 Series User Guide...
  • Page 58 See Accessories for details. The status of accessories can be monitored during measurements. Related Topics Accessories Setting Application Parameters Fixed Instrument Status Monitors Instrument Tab for Fixed Samples Tab for Fixed Evolution 200 Series User Guide Thermo Scientific...
  • Page 59 If the table contains just one sample and data is acquired with an accessory that automatically changes samples, sample names are incremented. Related Topics Setting Application Parameters Fixed Instrument Tab for Fixed Accessories Tab for Fixed Thermo Scientific Evolution 200 Series User Guide...

  • Page 60: Scan

    Data interval Difference in wavelength between two consecutive data points. Scan speed Wavelength range covered per unit time. Varies inversely with integration time. Increases as the data interval increases. Evolution 200 Series User Guide Thermo Scientific...
  • Page 61 The parameters for a selected operation appear to the right. See Finding Peaks in Scan Data Finding Value Level Crossings in Scan Data for information on setting them and how results are displayed. Related Topics Setting Application Parameters Scan Thermo Scientific Evolution 200 Series User Guide...
  • Page 62 For more information, see ASTM method E903. The calculation in transmission performed in the instrument is: S - 0%T S - 0%R --------------------------------- - ---------------------------------- - 100%T - 0%T 100%R - 0%R Requires a previously acquired 100%T baseline. Evolution 200 Series User Guide Thermo Scientific...
  • Page 63 Saves in a .tsv file, .csv file, or .xml (extensible markup language) file the wavelength and intensity values in the table. Related Topics Setting Application Parameters Scan Instrument Tab for Scan Measurement Tab for Scan Samples Tab for Scan Thermo Scientific Evolution 200 Series User Guide...
  • Page 64 Number of samples (see above) is set to 2 or greater: 1. Select Increment sample name from selected row. 2. Click the cell in the Sample Name column for starting the sequence. 3. Type a new sample name. 4. Press Enter. Evolution 200 Series User Guide Thermo Scientific...

  • Page 65: Quant

    To set parameters that define a Quant template, click Settings to access: Type Tab for Quant Measurement Tab for Quant Standards Tab for Quant Instrument Tab for Quant Accessories Tab for Quant Samples Tab for Quant Thermo Scientific Evolution 200 Series User Guide...
  • Page 66 Quant Type Type of quantitative analysis (see the table below). An image representing the selected type appears to the right. The required tabs also appear (click the Reset action button to step through them). Evolution 200 Series User Guide Thermo Scientific...
  • Page 67 Quant to display the Measurement tab in the right pane. The available features depend on the Quant Type setting on the Type tab. The features for each type are described in tables below. Thermo Scientific Evolution 200 Series User Guide...
  • Page 68 Use extinction coefficient Calculates component concentration with absorbance (A) equal ε where ε is the entered extinction coefficient (a constant dependent on the component and wavelength), c is concentration in the selected unit, and is 1 cm (the pathlength). Evolution 200 Series User Guide Thermo Scientific...
  • Page 69 3. Click OK. 4. For selected provided formulas, enter a name and concentration unit in the table. The available units depend on the Formulas & Units tab settings. Measure single standard Thermo Scientific Evolution 200 Series User Guide...
  • Page 70 Wavelengths to use for the quantitative analysis. Correction Correction above. Component name Component to quantify. Unit Component concentration unit. The available units depend on the Formulas & Units tab settings. Calculate additional Calculate additional results above. results Evolution 200 Series User Guide Thermo Scientific...
  • Page 71 Formulas & Units settings. 3. Click OK. Component name Component to quantify. Unit Component concentration unit. The available units depend on Formulas & Units tab settings in Options. Calculate additional Calculate additional results above. results Thermo Scientific Evolution 200 Series User Guide...
  • Page 72 The tables below describe the operations and functions usable in Quant equations specified on the Measurement tab. Arithmetic operations (including functions) are performed first, followed by comparison operations, and finally logical operations. Note The operators, arguments and functions are not case-sensitive. Evolution 200 Series User Guide Thermo Scientific...
  • Page 73 If either side of the OR is true, result is true 5 > 4 OR 2 = 3 true The following equation functions produce a value based on one or more arguments you supply. Thermo Scientific Evolution 200 Series User Guide...
  • Page 74 Result of the constant e (2.7182818) raised to the power of the specified number (e^<number>). ln(<number>) Natural logarithm (base e logarithm) of a number. log10(<number>) Base 10 logarithm of a specified number. log(<number>) pow(<value>, <power>) Value raised to the power. Example: pow(2,3) is 8.0 Evolution 200 Series User Guide Thermo Scientific...
  • Page 75 Index of the first occurrence of the <search> text in the <text>) specified text. If the text is not found, a -1 is returned. Lowercase(<text>) Copy of the text converted to lower case using the casing rules of the current culture. Thermo Scientific Evolution 200 Series User Guide...
  • Page 76 Location (X-axis value) at which the maximum Y-axis value occurs between two spectral limits. PLoc(start, end, Corrects the spectrum with a one-point baseline and then report the baseline) location (X-axis value) at which the maximum Y-axis value occurs between two spectral limits. Evolution 200 Series User Guide Thermo Scientific...
  • Page 77 Standards Tab for Quant To display the Standards tab in the right pane, click Settings in Quant and then set Quant Type to Standard curve, Standard curve with two wavelengths, or Advanced standard curve. Thermo Scientific Evolution 200 Series User Guide...
  • Page 78 Related Topics Quant Type Tab for Quant Measurement Tab for Quant Instrument Tab for Quant Samples Tab for Quant Accessories Tab for Quant Formulas and Units Tab Quantifying Sample Data Performing a Quantitative Analysis Evolution 200 Series User Guide Thermo Scientific...
  • Page 79 Estimated duration of data acquisition. Increases as integration only) time increases and as the data interval decreases. Varies inversely with scan speed. Table of wavelengths Shows the analysis wavelength(s) entered on the Measurement (Fixed only) tab. Related Topics Quant Thermo Scientific Evolution 200 Series User Guide...
  • Page 80 Instrument Status Monitors Type Tab for Quant Measurement Tab for Quant Standards Tab for Quant Instrument Tab for Quant Samples Tab for Quant Formulas and Units Tab Quantifying Sample Data Performing a Quantitative Analysis Evolution 200 Series User Guide Thermo Scientific...
  • Page 81 Related Topics Quant Type Tab for Quant Measurement Tab for Quant Standards Tab for Quant Instrument Tab for Quant Accessories Tab for Quant Formulas and Units Tab Quantifying Sample Data Performing a Quantitative Analysis Thermo Scientific Evolution 200 Series User Guide...

  • Page 82: Rate

    Type of kinetics measurement to perform. Related Topics Measurement Tab for Rate Instrument Tab for Rate Accessories Tab for Rate Samples Tab for Rate Rate Calculations Tab for Rate Performing a Kinetics Measurement Evolution 200 Series User Guide Thermo Scientific...
  • Page 83 In a multicell experiment the interval setting is the measurement interval for each sample. For single and multiple wavelength experiments only. Cycle time Time between each measurement cycle. For scan experiments only. Thermo Scientific Evolution 200 Series User Guide...
  • Page 84 A larger spectral bandwidth increases the amount of energy that passes through the sample. Increasing the bandwidth can improve the signal-to-noise ratio; however, a larger spectral bandwidth decreases the ability to resolve closely spaced spectral features, especially in scanning measurements. Evolution 200 Series User Guide Thermo Scientific...
  • Page 85 The available parameters depend on the installed accessories. See Accessories for details. The status of accessories can be monitored during measurements. Related Topics Accessories Instrument Status Monitors Type Tab for Rate Measurement Tab for Rate Thermo Scientific Evolution 200 Series User Guide...
  • Page 86 Rate Calculations Tab for Rate Performing a Kinetics Measurement Rate Calculations Tab for Rate Click Settings in Rate to display the Rate Calculations tab in the right pane (for single and multiple wavelength experiments only). Evolution 200 Series User Guide Thermo Scientific...

  • Page 87: Sharing Custom Templates

    To share a custom template, paste a copy of it into the desired folder, such as: C:\Users\Public\Documents\Thermo\Insight\Custom Methods C:\Documents and Settings\All Users\Documents\Thermo\Insight\Custom Methods Note “Shared Documents” may appear in a path instead of “Documents”. Thermo Scientific Evolution 200 Series User Guide...

  • Page 88: Managing Data

    Use the links below the buttons to display archived workbooks. Click Active workbook library to view the directory where a workbook was last saved. To change the path to the default workbook, right-click My favorite workbook library and choose Edit Link. Related Topics Managing Data Evolution 200 Series User Guide Thermo Scientific...

  • Page 89: Reports

    Separated Values (*.tsv) wavelengths for highlighted samples in a format that can be opened in Notepad or Excel. Multiple samples are saved sequentially in one column. These tabs appear in the Reports right pane: Thermo Scientific Evolution 200 Series User Guide...
  • Page 90 Title and subtitle to appear in the header. Use the Font buttons to specify their fonts. Table Includes a table of sample information. Use the Font button to specify the font for the information. Evolution 200 Series User Guide Thermo Scientific...

  • Page 91: Math And Analysis Operations

    Finding Value Level Crossings in Scan Data Smoothing Scan Data Use Smooth in the Math menu to reduce noise in wavelength scan data. The smoothing algorithm uses the Savitzky-Golay method. For more information, see About Smoothing. Thermo Scientific Evolution 200 Series User Guide...
  • Page 92 To add the result to the list without saving it, click Temporary. Asterisks at the beginning of a spectrum name indicate the data is not saved. 7. Click Close to close each displayed math window. Evolution 200 Series User Guide Thermo Scientific...
  • Page 93 Shift or Ctrl. Selected spectra appear in the lower-right pane. c. Click OK. Each added spectrum is listed with asterisks at the beginning of its name to indicate the data is not in the current workbook. Thermo Scientific Evolution 200 Series User Guide...
  • Page 94 8. Click Close to close each displayed math window. Related Topics Smoothing Scan Data Converting Scan Data to Other Units Adding Two Scan Spectra Normalizing Scan Data Subtracting a Scan Spectrum Ratioing Two Scan Spectra Evolution 200 Series User Guide Thermo Scientific...
  • Page 95 To return to the previous window, click Back. To perform additional math operations, repeat this step. 5. To save the result in the current workbook, click Workbook and name the spectrum or spectra when prompted. Thermo Scientific Evolution 200 Series User Guide...
  • Page 96 2. Select the second spectrum to add and click Add in the Operand 2 box. 3. To multiply the second spectrum by a factor other than 1, drag the slider or use Factor. Evolution 200 Series User Guide Thermo Scientific...
  • Page 97 Subtracting a Scan Spectrum Ratioing Two Scan Spectra Averaging Scan Spectra Adding, Subtracting, Multiplying or Dividing Scan Data by a Factor Finding Peaks in Scan Data Finding Value Level Crossings in Scan Data Thermo Scientific Evolution 200 Series User Guide...
  • Page 98 The data is added to the list and is available for operations. To add the result to the list without saving it, click Temporary. Asterisks at the beginning of a spectrum name indicate the data is not saved. Evolution 200 Series User Guide Thermo Scientific...
  • Page 99 2. Select the spectrum to subtract and click Add in the Operand 2 box. 3. To multiply the second spectrum by a factor other than 1, drag the slider or use Factor. Thermo Scientific Evolution 200 Series User Guide...
  • Page 100 Adding Two Scan Spectra Ratioing Two Scan Spectra Averaging Scan Spectra Adding, Subtracting, Multiplying or Dividing Scan Data by a Factor Finding Peaks in Scan Data Finding Value Level Crossings in Scan Data Evolution 200 Series User Guide Thermo Scientific...
  • Page 101 The data is added to the list and is available for operations. To add the result to the list without saving it, click Temporary. Asterisks at the beginning of a spectrum name indicate the data is not saved. Thermo Scientific Evolution 200 Series User Guide...

  • Page 102: Averaging Scan Spectra

    Note A spectrum appears in the Result pane only if two or more spectra are in the Operands pane. 3. To perform a math operation on the result, click Operate on Result and choose an operation. Otherwise, go to the next step. Evolution 200 Series User Guide Thermo Scientific...

  • Page 103: Adding, Subtracting, Multiplying Or Dividing Scan Data By A Factor

    Use Factor in the Math menu to add a constant to the Y value of scan data, subtract a constant, multiply by a constant or divide by a constant. To use Factor 1. Choose Math (menu) > Factor. Thermo Scientific Evolution 200 Series User Guide...
  • Page 104 The data is added to the list and is available for operations. To add the result to the list without saving it, click Temporary. Asterisks at the beginning of a spectrum name indicate the data is not saved. Evolution 200 Series User Guide Thermo Scientific...

  • Page 105: Finding Peaks In Scan Data

    Order for labeling and listing found items in the results peaks and valleys) by table. Sensitivity How readily small peaks or valleys are found. To optimize the sensitivity, select Auto. To set it manually, deselect Auto and drag the slider. Thermo Scientific Evolution 200 Series User Guide...
  • Page 106 To adjust the data display, use the view finder. To zoom in or move the spectrum, use the selection tool: The results appear in the data display pane and the table below it: Evolution 200 Series User Guide Thermo Scientific...

  • Page 107: Finding Value Level Crossings In Scan Data

    Finding Value Level Crossings in Scan Data Finding Value Level Crossings in Scan Data Use Value Level in the Analyze menu to find the wavelengths where a spectrum crosses a specified ordinate value. Thermo Scientific Evolution 200 Series User Guide...
  • Page 108 To adjust the data display, use the view finder. To zoom in or move the spectrum, use the selection tool: The results appear in the data display pane and the table below it: Evolution 200 Series User Guide Thermo Scientific...
  • Page 109 Converting Scan Data to Other Units Adding Two Scan Spectra Subtracting a Scan Spectrum Ratioing Two Scan Spectra Averaging Scan Spectra Adding, Subtracting, Multiplying or Dividing Scan Data by a Factor Finding Peaks in Scan Data Thermo Scientific Evolution 200 Series User Guide...

  • Page 110: Options

    Drag the List of Templates application to one of the top nine menu buttons. Report Master Page Tab Click Options to display the Report Master Page tab in the right pane. Use it to define the appearance of all printed reports. Evolution 200 Series User Guide Thermo Scientific...

  • Page 111: Preferences Tab

    Type a path for each or click the button to specify a path. Data Store Tab Click Options to display the Data Store tab in the right pane. Use it to specify how to save workbooks, export data and e-mail results. Thermo Scientific Evolution 200 Series User Guide...

  • Page 112: Formulas And Units Tab

    To delete an item, right-click it and choose Delete Row. Items with a lock icon cannot be edited or deleted. To return to the default selections, click Reset. Related Topics Quanto Quantifying Sample Data Performing a Quantitative Analysis Evolution 200 Series User Guide Thermo Scientific...

  • Page 113: System Settings

    2. Set Bandwidth as desired. The available settings depend on the instrument. 3. Click Start. Every half second the sample and reference detector intensities are plotted and displayed by the Sample beam and Reference readouts. Thermo Scientific Evolution 200 Series User Guide...

  • Page 114: Calibrations Tab

    To calibrate using a particular lamp, click its Calibrate button and follow the instructions that appear. See Mercury Lamp for instructions for using a mercury lamp safely. Evolution 200 Series User Guide Thermo Scientific...

  • Page 115: Lamp Tab

    Instrument Boot Click Display to view the instrument’s power-up boot record, which Record can be saved or copied. Related Topics Alignment Tab Calibrations Tab Lamp Tab Keyboard Shortcuts These are available in INSIGHT software: Thermo Scientific Evolution 200 Series User Guide...
  • Page 116 Acquires zero or baseline data. Chooses the Measure button. Prints the Measure screen. Prints the report. Displays My Data. Creates a new workbook. F11 (from Home) Displays Performance Verification. Chooses the Stop button. Evolution 200 Series User Guide Thermo Scientific...

  • Page 117: Evolution 200 Series Spectrophotometers

    Languages WARNING n'utilisez le système qu'en suivant les consignes de sécurité qui figurent dans ce manuel et dans la documentation accompagnant le système. Thermo Scientific Evolution 200 Series User Guide...

  • Page 118: Safety Label Locations

    Safety Label Locations This section shows the locations of safety labels on the spectrophotometer. Contact us if there are questions or problems with the safety labels. See Contacting Back view Compliance label Evolution 200 Series User Guide Thermo Scientific...
  • Page 119 Right side view UV radiation warning label (under mercury lamp access cover) Left side view UV radiation warning label (under detector access cover) Related Topics Mercury Lamp Removing and Installing the Detector Module Thermo Scientific Evolution 200 Series User Guide...

  • Page 120: Lifting Or Moving The Instrument

    • Connectors • Removable Panels • Corrosion Protection Spectrophotometer Components The following illustration identifies major components visible on the outside of a typical spectrophotometer. (Some components may not be present on all instruments.) Evolution 200 Series User Guide Thermo Scientific...

  • Page 121: Spectrophotometer Basics

    To open the sample compartment, press the button on its door. To close the compartment, slide the door forward until it latches. To access the reference compartment, lift or lower the door. Related Topics Sample Compartment Front Panel Tubing Access Panel Thermo Scientific Evolution 200 Series User Guide...

  • Page 122: Insight Software

    The touchscreen is included only on Local Control versions of the spectrophotometer. Related Topics Touchscreen keyboard Cleaning the Touchscreen Connectors This section shows the locations of the connectors inside and outside the spectrophotometer. Evolution 200 Series User Guide Thermo Scientific...

  • Page 123: Outside Cover

    Computer Control Instruments. Hg lamp Connects to optional Mercury Lamp. Power Connects to main power cord. Power switch System power On/Off switch. Purge Connects to optional purge source. See Purging the Sample Compartment. Thermo Scientific Evolution 200 Series User Guide...
  • Page 124 USB cable connected to the instrument. Loop the cable once and clamp the ring over the loop as shown below. Ferrite ring Note The spectrophotometer does not support high power USB devices. Related Topics Triggering Evolution 200 Series User Guide Thermo Scientific...

  • Page 125: Removable Panels

    This section covers panels that can be removed to allow access to features on the instrument. Contents • Sample Compartment Front Panel • Tubing Access Panel • Mercury Lamp Access Cover • Detector Access Cover • Detector Access Panel Thermo Scientific Evolution 200 Series User Guide...

  • Page 126: Tubing Access Panel

    Installing a Manual Accessory Installing a Liquid Thermostatted Accessory Installing a Smart Accessory Tubing Access Panel To access external ports for routing fluid from a recirculator to the instrument, remove the panel below the sample compartment. Evolution 200 Series User Guide Thermo Scientific...

  • Page 127: Mercury Lamp Access Cover

    To remove the panel, loosen the screw. Related Topics Installing a Liquid Thermostatted Accessory Mercury Lamp Access Cover WARNING Avoid UV radiation hazard in the mercury lamp compartment. Protect eyes and skin from exposure. Languages Thermo Scientific Evolution 200 Series User Guide...

  • Page 128: Detector Access Cover

    Detector Access Cover Some accessories require removing the instrument detector module. The module is beneath the detector access cover shown below. Avoid UV radiation hazard in the detector compartment. Protect eyes and skin from exposure. Evolution 200 Series User Guide Thermo Scientific...
  • Page 129 único equipo que proporciona la protección para los ojos adecuada contra la luz ultravioleta directa y reflejada Left side view Detector access cover Related Topics Removing and Installing the Detector Module Detector Access Panel Thermo Scientific Evolution 200 Series User Guide...

  • Page 130: Detector Access Panel

    Related Topics Installing Sample Compartment Windows Purging the Sample Compartment Installing Sample Compartment Windows The optional windows attach to the sample compartment side walls using magnets. Evolution 200 Series User Guide Thermo Scientific...
  • Page 131 Use dry air or dry nitrogen to reduce or eliminate condensation. The purge gas must be free of moisture, oil and other reactive materials. To remove particulate matter and oil, install a 10-micrometer filter. WARNING Never use a flammable gas to purge the instrument. Languages Thermo Scientific Evolution 200 Series User Guide...

  • Page 132: Standard Cell Holders

    Standard Cell Holders The instrument includes one sample and one reference cell holder. For a list of optional cell holders and other accessories, see Accessories. Related Topics 10 mm Rectangular Sample Cell Holder Evolution 200 Series User Guide Thermo Scientific...

  • Page 133: Rectangular Reference Cell Holder

    For measurements with a reference sample, install a reference cell holder in the reference position. See Installing a Reference Cell Holder. The standard reference holder accommodates rectangular reference cells with these pathlengths: 10 mm, 20 mm, 40 mm, 50 mm and 100 mm. Thermo Scientific Evolution 200 Series User Guide...

  • Page 134: Installing A Manual Accessory

    It is not necessary to power off the instrument while installing or removing a manual accessory. Sample compartment top view Alignment pins Toggle To install a manual accessory 1. Open the sample compartment door. See Opening and Closing the Sample and Reference Compartment Doors. Evolution 200 Series User Guide Thermo Scientific...

  • Page 135: Installing A Smart Accessory

    These accessories install in the sample compartment. A connector under the accessory baseplate provides data communication and power. It is not necessary to power off the instrument while installing or removing a Smart Accessory. Thermo Scientific Evolution 200 Series User Guide...
  • Page 136 Grasp the accessory by the handles and lower it into the sample compartment aligning the connector under the baseplate with the connector in the sample compartment floor. Use the alignment pins on either side of the connector as guides. Evolution 200 Series User Guide Thermo Scientific...
  • Page 137 Initialisierung vom Zubehör fern zu halten. CAUTELA Evitare il rischio di pizzicamento. Tenere mani e oggetti lontano dall’accessorio durante l’inizializzazione. PRECAUCIÓN Evite riesgos de atrapamiento. Mantenga manos y objetos lejos del alcance del accesorio durante la inicialización. Thermo Scientific Evolution 200 Series User Guide...

  • Page 138: Installing A Liquid Thermostatted Accessory

    The Recirculator Tubing Kit provides parts for connecting an accessory to a fluid recirculator. To install a thermostatted accessory 1. Install the accessory. Installing a Manual Accessory Installing a Smart Accessory. 2. Remove the tubing access panel. See Tubing Access Panel. Evolution 200 Series User Guide Thermo Scientific...
  • Page 139 Note Ensure the tubing does not obstruct the light beam or prevent the sample compartment door from closing. If necessary, secure the tubing using the supplied clips. Related Topics Installing a Smart Accessory Installing a Manual Accessory Installing a Reference Cell Holder Thermo Scientific Evolution 200 Series User Guide...

  • Page 140: Installing A Reference Cell Holder

    Contents • Computer Control Instruments • Local Control Instruments Computer Control Instruments Computer Control instruments must be connected to an external computer with the proper software installed. Evolution 200 Series User Guide Thermo Scientific...

  • Page 141: Setting Up A Computer Control Instrument

    Turning off a Computer Control Instrument Local Control Instruments Turning off a Computer Control Instrument To turn off the instrument 1. Close INSIGHT software. 2. Hold down the Power button on the keypad for 4 seconds. Thermo Scientific Evolution 200 Series User Guide...

  • Page 142: Local Control Instruments

    Power button on the keypad to turn the instrument on and off. Related Topics Setting up a Local Control instrument for remote operation Turning off a Local Control Instrument Evolution 200 Series User Guide Thermo Scientific...

  • Page 143: Setting Up A Local Control Instrument For Remote Operation

    2. Double-click the INSIGHT desktop icon on the touchscreen to start the software. Related Topics Setting up a Local Control instrument for touchscreen operation Turning off a Local Control Instrument Computer Control Instruments Thermo Scientific Evolution 200 Series User Guide...

  • Page 144: Keypad Operation

    Turn the instrument on or off (press and hold 4 seconds to power off ). Function Start an assigned program or CUE script. See Assigning CUE Scripts to Function Keys. Use the CUE application to edit scripts. See the CUE on-line help for details. Evolution 200 Series User Guide Thermo Scientific...

  • Page 145: Keypad Shortcuts

    These compartments have a port to allow small amounts of liquid to drain from the instrument. NOTICE Do not allow moisture to leak into the instrument interior. To clean the touchscreen, see Cleaning the Touchscreen. Thermo Scientific Evolution 200 Series User Guide...

  • Page 146: Cleaning The Touchscreen

    Removing and Installing the Detector Module Some accessories have a dedicated detector and require removing the instrument detector module before they can be installed. Avoid UV radiation hazard in the detector compartment. Protect eyes and skin from exposure. Evolution 200 Series User Guide Thermo Scientific...
  • Page 147 Cover. 3. Loosen the thumbscrew that attaches the detector module to the instrument, and carefully lift the module straight up. NOTICE Avoid touching the connector pins on the bottom of the detector module. Thermo Scientific Evolution 200 Series User Guide...
  • Page 148 6. Tighten the thumbscrew by hand. 7. Replace the detector access cover. Related Topics Routine Maintenance Cleaning the Instrument Cleaning the Touchscreen Evolution 200 Series User Guide Thermo Scientific...

  • Page 149: Ordering Parts

    Evolution 200 Series Spectrophotometers Ordering Parts Detector Access Cover Detector Access Panel Ordering Parts To order replacement parts, see Contacting Thermo Scientific Evolution 200 Series User Guide...
  • Page 150 Evolution 200 Series Spectrophotometers Ordering Parts Evolution 200 Series User Guide Thermo Scientific...

  • Page 151: Life Science Applications

    Protein A280 Protein A280 Proteins and Labels Protein Bradford Pierce BCA Pierce Modified Lowry Pierce 660 nm Protein Assay Protein Biuret Below are the default values for the methods. Wavelengths are in nanometers. Thermo Scientific Evolution 200 Series User Guide...

  • Page 152: Nucleic Acid

    Use the Nucleic Acid application to measure nucleic acid samples for concentration and purity. Unique Screen Features for information about the results the application calculates. See Nucleic Acid Concentration Calculations for information about the calculations. Evolution 200 Series User Guide Thermo Scientific...

  • Page 153: Overview

    Related Topics Overview Application Settings Unique Screen Features Making Nucleic Acid Measurements Oligo Calculator Nucleic Acid Purity Measurements The 260/280 nm ratio assay determines the purity of nucleic acids based on the 260/280 ratio. Thermo Scientific Evolution 200 Series User Guide...

  • Page 154: Application Settings

    Making Nucleic Acid Measurements Oligo Calculator Type Tab for Nucleic Acid Click Settings in Nucleic Acid to display the Type tab in the right pane. Feature Description Name Application name. Description (optional) Description of the template. Evolution 200 Series User Guide Thermo Scientific...
  • Page 155 Sloping baseline draws a line between the data points at the entered wavelengths, finds its absorbance value at the analytical wavelength, and subtracts it from the measured absorbance. Component name Component to quantify. Thermo Scientific Evolution 200 Series User Guide...
  • Page 156 4. For selected provided formulas, enter a name and concentration unit in the table. The available units depend on the Formulas & Units tab settings. Related Topics Type Tab for Nucleic Acid Instrument Tab for Nucleic Acid Evolution 200 Series User Guide Thermo Scientific...
  • Page 157 Shows the analysis wavelength(s) entered on the Measurement (Fixed only) tab. Related Topics Type Tab for Nucleic Acid Measurement Tab for Nucleic Acid Accessories Tab for Nucleic Acid Samples Tab for Nucleic Acid Thermo Scientific Evolution 200 Series User Guide...
  • Page 158 Displays high and low limit lines on the Run Chart tab to show limits whether sample concentrations are within the specified limits. Related Topics Type Tab for Nucleic Acid Measurement Tab for Nucleic Acid Instrument Tab for Nucleic Acid Evolution 200 Series User Guide Thermo Scientific...

  • Page 159: Unique Screen Features

    Concentration determined by multiplying the absorbance at 260 nm by a factor (default, 50) after correction is applied. Beer’s law is used to calculate the nucleic acid concentration (see Nucleic Acid Concentration Calculations). A260 Absorbance at 260 nm. Thermo Scientific Evolution 200 Series User Guide...

  • Page 160: Making Nucleic Acid Measurements

    Use the appropriate buffer for the blank, generally the same buffer used to prepare or dissolve the sample. The buffer should be the same pH and of a similar ionic strength as the sample solution. Evolution 200 Series User Guide Thermo Scientific...
  • Page 161 To measure a sample again, right-click its row in the results table and choose Remeasure. After the remeasurement, the previous information for the sample is crossed out (but not removed from the table). Thermo Scientific Evolution 200 Series User Guide...

  • Page 162: Oligo Calculator

    2. Select the nucleic acid type to analyze. 3. Select the degree of phosphorylation if applicable: mono-phosphate for DNA; mono- or tri-phosphate for RNA. 4. Select Double-stranded if applicable. The complementary base sequence will be included in the analysis. Evolution 200 Series User Guide Thermo Scientific...

  • Page 163: Nucleic Acid Labels

    This method does not account for the effect of interaction between neighboring bases. Nearest-neighbor Melting point of the base sequence when the effect of interaction between neighboring bases is taken into account. Nucleic Acid Labels Contents Thermo Scientific Evolution 200 Series User Guide...

  • Page 164: Overview

    260 nm corrections will be used for nucleic acid sample concentration calculations. Entered information is saved automatically. To delete a user-defined dye, right-click its row and choose Delete Row. Predefined dyes, identified by the lock icon, cannot be edited or deleted. Evolution 200 Series User Guide Thermo Scientific...

  • Page 165: Application Settings

    The factor used for other types cannot be changed. Set Dye 1 and Dye 2 to the fluorescent dyes whose absorbance will be used in concentration correction calculations. Pathlength Distance the light travels through the sample. Thermo Scientific Evolution 200 Series User Guide...
  • Page 166 Sloping baseline draws a line between the data points at the entered wavelengths, finds its absorbance value at the analytical wavelength, and subtracts it from the measured absorbance. Component name Component to quantify. Evolution 200 Series User Guide Thermo Scientific...
  • Page 167 4. For selected provided formulas, enter a name and concentration unit in the table. The available units depend on the Formulas & Units tab settings. Related Topics Type Tab for Nucleic Acid Labels Instrument Tab for Nucleic Acid Labels Thermo Scientific Evolution 200 Series User Guide...
  • Page 168 Shows the analysis wavelength(s) entered on the Measurement (Fixed only) tab. Related Topics Type Tab for Nucleic Acid Labels Measurement Tab for Nucleic Acid Labels Accessories Tab for Nucleic Acid Labels Samples Tab for Nucleic Acid Labels Evolution 200 Series User Guide Thermo Scientific...

  • Page 169: Accessories Tab For Nucleic Acid Labels

    Displays high and low limit lines on the Run Chart tab to show limits whether sample concentrations are within the specified limits. Related Topics Type Tab for Nucleic Acid Labels Measurement Tab for Nucleic Acid Labels Thermo Scientific Evolution 200 Series User Guide...

  • Page 170: Unique Screen Features

    280 nm may be present. See Nucleic Acid Purity Measurements more information. Dye(s) Conc. Concentration of the user-selected dye(s). Related Topics Overview Dye/Chromophore Editor Application Settings Making Nucleic Acid Labels Measurements Oligo Calculator Evolution 200 Series User Guide Thermo Scientific...

  • Page 171: Making Nucleic Acid Labels Measurements

    The Run Chart tab plots the concentration of the measured component versus sample number. If Use concentration limits was selected on the Samples tab, horizontal limit lines show whether the concentrations are within the specified limits: Thermo Scientific Evolution 200 Series User Guide...
  • Page 172 Related Topics Overview Dye/Chromophore Editor Application Settings Unique Screen Features Data Display Oligo Calculator After the Measurement • When using the cuvette option, remove the cuvette, rinse thoroughly and dry between samples. Evolution 200 Series User Guide Thermo Scientific...

  • Page 173: Oligo Calculator

    Molecular weight Calculated base sequence molecular weight. Molar extinction Calculated 260 nm extinction coefficient in ng-cm/microliter. coefficient Concentration factor Factor, based on the extinction coefficient, used to calculate the concentration of the base sequence. Thermo Scientific Evolution 200 Series User Guide...

  • Page 174: Protein A280

    • Overview • Application Settings • Unique Screen Features • Making Protein A280 Measurements Overview The Protein A280 method is used to quantify proteins on the basis of their absorbance at 280 nm. Evolution 200 Series User Guide Thermo Scientific...

  • Page 175: Application Settings

    Making Protein A280 Measurements Type Tab for Protein A280 Click Settings in Protein A280 to display the Type tab in the right pane. Feature Description Name Application name. Description (optional) Description of the template. Thermo Scientific Evolution 200 Series User Guide...
  • Page 176 Instrument Tab for Protein A280 Accessories Tab for Protein A280 Samples Tab for Protein A280 Measurement Tab for Protein A280 Click Settings in Protein A280 to display the Measurement tab in the right pane. Evolution 200 Series User Guide Thermo Scientific...
  • Page 177 Sloping baseline draws a line between the data points at the entered wavelengths, finds its absorbance value at the analytical wavelength, and subtracts it from the measured absorbance. Component name Component to quantify. Thermo Scientific Evolution 200 Series User Guide...
  • Page 178 4. For selected provided formulas, enter a name and concentration unit in the table. The available units depend on the Formulas & Units tab settings. Related Topics Type Tab for Protein A280 Instrument Tab for Protein A280 Evolution 200 Series User Guide Thermo Scientific...
  • Page 179 Shows the analysis wavelength(s) entered on the Measurement (Fixed only) tab. Related Topics Type Tab for Protein A280 Measurement Tab for Protein A280 Accessories Tab for Protein A280 Samples Tab for Protein A280 Thermo Scientific Evolution 200 Series User Guide...
  • Page 180 Displays high and low limit lines on the Run Chart tab to show limits whether sample concentrations are within the specified limits. Related Topics Type Tab for Protein A280 Measurement Tab for Protein A280 Instrument Tab for Protein A280 Evolution 200 Series User Guide Thermo Scientific...

  • Page 181: Unique Screen Features

    1. Click Measure Protein A280 in Protein A280. Note To start an analysis immediately after viewing or changing the template settings, click the Measure action button instead and skip to step 3. 2. Click Measure. Thermo Scientific Evolution 200 Series User Guide...
  • Page 182 If Use concentration limits was selected on the Samples tab, horizontal limit lines show whether the concentrations are within the specified limits: To copy this plot, right-click it and choose Copy to Clipboard. Evolution 200 Series User Guide Thermo Scientific...

  • Page 183: Proteins And Labels

    280 nm as well as the concentration of a fluorescent dye modification (protein array conjugates). It can also measure the purity of metalloproteins (such as hemoglobin) using wavelength ratios. Related Topics Dye/Chromophore Editor Thermo Scientific Evolution 200 Series User Guide...

  • Page 184: Overview

    Related Topics Overview Dye/Chromophore Editor Unique Screen Features Making Proteins and Labels Measurements Type Tab for Proteins and Labels Click Settings in Proteins and Labels to display the Type tab in the right pane. Evolution 200 Series User Guide Thermo Scientific...
  • Page 185 Distance the light travels through the sample. Related Topics Measurement Tab for Proteins and Labels Instrument Tab for Proteins and Labels Accessories Tab for Proteins and Labels Samples Tab for Proteins and Labels Thermo Scientific Evolution 200 Series User Guide...
  • Page 186 Sloping baseline draws a line between the data points at the entered wavelengths, finds its absorbance value at the analytical wavelength, and subtracts it from the measured absorbance. Component name Component to quantify. Evolution 200 Series User Guide Thermo Scientific...
  • Page 187 4. For selected provided formulas, enter a name and concentration unit in the table. The available units depend on the Formulas & Units tab settings. Related Topics Type Tab for Proteins and Labels Thermo Scientific Evolution 200 Series User Guide...
  • Page 188 Table of wavelengths Shows the analysis wavelength(s) entered on the Measurement (Fixed only) tab. Related Topics Type Tab for Proteins and Labels Measurement Tab for Proteins and Labels Accessories Tab for Proteins and Labels Evolution 200 Series User Guide Thermo Scientific...

  • Page 189: Accessories Tab For Proteins And Labels

    Triplicate, respectively. Use concentration Displays high and low limit lines on the Run Chart tab to show limits whether sample concentrations are within the specified limits. Related Topics Type Tab for Proteins and Labels Thermo Scientific Evolution 200 Series User Guide...

  • Page 190: Unique Screen Features

    1. Click Measure Proteins & Labels in Proteins and Labels. Note To start an analysis immediately after viewing or changing the template settings, click the Measure action button instead and skip to step 3. Evolution 200 Series User Guide Thermo Scientific...
  • Page 191 If Use concentration limits was selected on the Samples tab, horizontal limit lines show whether the concentrations are within the specified limits: To copy this plot, right-click it and choose Copy to Clipboard. Thermo Scientific Evolution 200 Series User Guide...

  • Page 192: Pierce Bca

    Related Topics Overview Dye/Chromophore Editor Application Settings Unique Screen Features Data Display Pierce BCA Contents • Overview • Application Settings • Working With Standard Curves • Making Pierce BCA Measurements Evolution 200 Series User Guide Thermo Scientific...

  • Page 193: Overview

    Note If running the assay at 60 °C, doubling the volumes may afford greater insurance against skewed results from evaporation/condensation within the sealed reaction tube. Protein standards (BSA) for generating a standard curve may also be provided by the manufacturer for the BCA assay. Thermo Scientific Evolution 200 Series User Guide...

  • Page 194: Application Settings

    Distance the light travels through the sample. Related Topics Measurement Tab for Pierce BCA Standards Tab for Pierce BCA Instrument Tab for Pierce BCA Accessories Tab for Pierce BCA Samples Tab for Pierce BCA Evolution 200 Series User Guide Thermo Scientific...
  • Page 195 Sloping baseline draws a line between the data points at the entered wavelengths, finds its absorbance value at the analytical wavelength, and subtracts it from the measured absorbance. Component name Component to quantify. Thermo Scientific Evolution 200 Series User Guide...
  • Page 196 4. For selected provided formulas, enter a name and concentration unit in the table. The available units depend on the Formulas & Units tab settings. Related Topics Type Tab for Pierce BCA Standards Tab for Pierce BCA Evolution 200 Series User Guide Thermo Scientific...

  • Page 197: Standards Tab For Pierce Bca

    .csv file or .tsv file, click Export Standards. Related Topics Type Tab for Pierce BCA Measurement Tab for Pierce BCA Instrument Tab for Pierce BCA Accessories Tab for Pierce BCA Thermo Scientific Evolution 200 Series User Guide...

  • Page 198: Instrument Tab For Pierce Bca

    Shows the analysis wavelength(s) entered on the Measurement (Fixed only) tab. Related Topics Type Tab for Pierce BCA Measurement Tab for Pierce BCA Standards Tab for Pierce BCA Accessories Tab for Pierce BCA Samples Tab for Pierce BCA Evolution 200 Series User Guide Thermo Scientific...

  • Page 199: Accessories Tab For Pierce Bca

    If available for the current unit, corrects sample concentrations using correction the entered target weight and volume: corrected concentration = measured concentration * (actual weight / target weight) * (target volume / actual volume) Thermo Scientific Evolution 200 Series User Guide...

  • Page 200: Working With Standard Curves

    • See the manufacturers’ guidelines and recommendations for standard and sample preparation. • Prepare both standards and unknowns in the same manner. Use a diluent of the same pH and ionic strength for all blanks, standards and unknown samples. Evolution 200 Series User Guide Thermo Scientific...
  • Page 201 To remove a standard, select it, right-click the table and choose Clear Standard. To remove all the standards, choose Clear Table. To save standards information for later use, use Export Standards. 5. Click Continue. 6. Follow the instructions that appear, installing the specified standards. Thermo Scientific Evolution 200 Series User Guide...
  • Page 202 8. Follow the instructions that appear. 9. When samples information appears, modify it if desired. If Calculate from weight/volume was selected on the Standards tab, enter the weight and volume for each sample in the table. More: Evolution 200 Series User Guide Thermo Scientific...
  • Page 203 The Run Chart tab plots the concentration of the measured component versus sample number. If Use concentration limits was selected on the Samples tab, horizontal limit lines show whether the concentrations are within the specified limits: Thermo Scientific Evolution 200 Series User Guide...
  • Page 204 • Application Settings • Bradford Standard Curves • Making Protein Bradford Assay Measurements Overview The Protein Bradford assay measures absorbance at 595 nm; it determines concentration for either standard or micro sample concentrations. Evolution 200 Series User Guide Thermo Scientific...

  • Page 205: Protein Bradford

    Measurement Tab for Protein Bradford Standards Tab for Protein Bradford Instrument Tab for Protein Bradford Accessories Tab for Protein Bradford Samples Tab for Protein Bradford Related Topics Overview Bradford Standard Curves Making Protein Bradford Assay Measurements Thermo Scientific Evolution 200 Series User Guide...
  • Page 206 Sloping baseline draws a line between the data points at the entered wavelengths, finds its absorbance value at the analytical wavelength, and subtracts it from the measured absorbance. Component name Component to quantify. Evolution 200 Series User Guide Thermo Scientific...
  • Page 207 4. For selected provided formulas, enter a name and concentration unit in the table. The available units depend on the Formulas & Units tab settings. Related Topics Type Tab for Protein Bradford Standards Tab for Protein Bradford Thermo Scientific Evolution 200 Series User Guide...
  • Page 208 .csv file or .tsv file, click Export Standards. Related Topics Type Tab for Protein Bradford Measurement Tab for Protein Bradford Instrument Tab for Protein Bradford Accessories Tab for Protein Bradford Evolution 200 Series User Guide Thermo Scientific...
  • Page 209 Shows the analysis wavelength(s) entered on the Measurement (Fixed only) tab. Related Topics Type Tab for Protein Bradford Measurement Tab for Protein Bradford Standards Tab for Protein Bradford Accessories Tab for Protein Bradford Samples Tab for Protein Bradford Thermo Scientific Evolution 200 Series User Guide...
  • Page 210 If available for the current unit, corrects sample concentrations using correction the entered target weight and volume: corrected concentration = measured concentration * (actual weight / target weight) * (target volume / actual volume) Evolution 200 Series User Guide Thermo Scientific...

  • Page 211: Bradford Standard Curves

    Note Each workbook will archive only one standard curve. Related Topics Overview Application Settings Bradford Standard Curves Making Protein Bradford Assay Measurements Making Protein Bradford Assay Measurements Sample and Standards Preparation for more information. Thermo Scientific Evolution 200 Series User Guide...
  • Page 212 The Standard Curve(s) tab displays the resulting standard curve (or curves). Specify the standards to use for the curve (or curves) by selecting Yes or No in the Use column in the table. More: Evolution 200 Series User Guide Thermo Scientific...
  • Page 213 If Sample averaging was set to Triplicate, “D” and “T” at the end of sample names indicate the second and third measurements to be made, respectively. Thermo Scientific Evolution 200 Series User Guide...
  • Page 214 The Run Chart tab plots the concentration of the measured component versus sample number. If Use concentration limits was selected on the Samples tab, horizontal limit lines show whether the concentrations are within the specified limits: Evolution 200 Series User Guide Thermo Scientific...

  • Page 215: Pierce Modified Lowry

    Folin-Ciocalteu Reagent is effectively reduced in proportion to the chelated copper-complexes, resulting in a water-soluble blue product that is measured at 650 nm and normalized at 405 nm. Preformulated reagents, used in the assay, are available in kit form from numerous manufacturers. Thermo Scientific Evolution 200 Series User Guide...

  • Page 216: Overview

    Making Pierce Modified Lowry Assay Measurements Type Tab for Pierce Modified Lowry Click Settings in Pierce Modified Lowry to display the Type tab in the right pane. Feature Description Name Application name. Description (optional) Description of the template. Evolution 200 Series User Guide Thermo Scientific...
  • Page 217 Sloping baseline draws a line between the data points at the entered wavelengths, finds its absorbance value at the analytical wavelength, and subtracts it from the measured absorbance. Component name Component to quantify. Thermo Scientific Evolution 200 Series User Guide...
  • Page 218 4. For selected provided formulas, enter a name and concentration unit in the table. The available units depend on the Formulas & Units tab settings. Related Topics Type Tab for Pierce Modified Lowry Standards Tab for Pierce Modified Lowry Evolution 200 Series User Guide Thermo Scientific...
  • Page 219 .csv file or .tsv file, click Export Standards. Related Topics Type Tab for Pierce Modified Lowry Measurement Tab for Pierce Modified Lowry Instrument Tab for Pierce Modified Lowry Accessories Tab for Pierce Modified Lowry Thermo Scientific Evolution 200 Series User Guide...
  • Page 220 Related Topics Type Tab for Pierce Modified Lowry Measurement Tab for Pierce Modified Lowry Standards Tab for Pierce Modified Lowry Accessories Tab for Pierce Modified Lowry Samples Tab for Pierce Modified Lowry Evolution 200 Series User Guide Thermo Scientific...
  • Page 221 If available for the current unit, corrects sample concentrations using correction the entered target weight and volume: corrected concentration = measured concentration * (actual weight / target weight) * (target volume / actual volume) Thermo Scientific Evolution 200 Series User Guide...

  • Page 222: Lowry Standard Curves

    Note Each workbook will archive only one standard curve. Related Topics Overview Application Settings Lowry Standard Curves Making Pierce Modified Lowry Assay Measurements Making Pierce Modified Lowry Assay Measurements Sample and Standards Preparation for more information. Evolution 200 Series User Guide Thermo Scientific...
  • Page 223 The Standard Curve(s) tab displays the resulting standard curve (or curves). Specify the standards to use for the curve (or curves) by selecting Yes or No in the Use column in the table. More: Thermo Scientific Evolution 200 Series User Guide...
  • Page 224 If Sample averaging was set to Triplicate, “D” and “T” at the end of sample names indicate the second and third measurements to be made, respectively. Evolution 200 Series User Guide Thermo Scientific...
  • Page 225 The Run Chart tab plots the concentration of the measured component versus sample number. If Use concentration limits was selected on the Samples tab, horizontal limit lines show whether the concentrations are within the specified limits: Thermo Scientific Evolution 200 Series User Guide...

  • Page 226: Pierce 660 Nm Protein Assay

    • Making Pierce 660 nm Protein Assay Measurements Overview The Thermo Scientific Pierce 660 nm Protein Assay reagent is a ready-to-use formulation that offers rapid, accurate and reproducible colorimetric detection of minute amounts of protein in solution. The reagent is ideal for measuring total protein concentration in samples containing reducing agents and/or detergents.

  • Page 227: Overview

    Standard Curves Making Pierce 660 nm Protein Assay Measurements Type Tab for Pierce 660 nm Protein Assay Click Settings in Pierce 660 nm Protein Assay to display the Type tab in the right pane. Thermo Scientific Evolution 200 Series User Guide...

  • Page 228: Measurement Tab For Pierce 660 Nm Protein Assay

    Sloping baseline draws a line between the data points at the entered wavelengths, finds its absorbance value at the analytical wavelength, and subtracts it from the measured absorbance. Component name Component to quantify. Evolution 200 Series User Guide Thermo Scientific...
  • Page 229 The available units depend on the Formulas & Units tab settings. Related Topics Type Tab for Pierce 660 nm Protein Assay Standards Tab for Pierce 660 nm Protein Assay Thermo Scientific Evolution 200 Series User Guide...

  • Page 230: Standards Tab For Pierce 660 Nm Protein Assay

    Related Topics Type Tab for Pierce 660 nm Protein Assay Measurement Tab for Pierce 660 nm Protein Assay Instrument Tab for Pierce 660 nm Protein Assay Accessories Tab for Pierce 660 nm Protein Assay Evolution 200 Series User Guide Thermo Scientific...

  • Page 231: Instrument Tab For Pierce 660 Nm Protein Assay

    Type Tab for Pierce 660 nm Protein Assay Measurement Tab for Pierce 660 nm Protein Assay Standards Tab for Pierce 660 nm Protein Assay Accessories Tab for Pierce 660 nm Protein Assay Samples Tab for Pierce 660 nm Protein Assay Thermo Scientific Evolution 200 Series User Guide...

  • Page 232: Accessories Tab For Pierce 660 Nm Protein Assay

    If available for the current unit, corrects sample concentrations using correction the entered target weight and volume: corrected concentration = measured concentration * (actual weight / target weight) * (target volume / actual volume) Evolution 200 Series User Guide Thermo Scientific...

  • Page 233: Pierce 660 Nm Assay Workbooks

    • If a previously saved workbook is used, all concentration calculations for newly measured samples will be based on the standard absorbance values saved in the workbook. Note Each workbook will archive only one standard curve. Thermo Scientific Evolution 200 Series User Guide...

  • Page 234: Making Pierce 660 Nm Protein Assay Measurements

    To enter previously saved standards information, use Import Standards. If Calculate from weight/volume was selected on the Standards tab, enter the weight and volume for each standard in the table. Evolution 200 Series User Guide Thermo Scientific...
  • Page 235 The Data tab displays the acquired data (a fixed data point or scan spectrum) for the standard selected in the table. To access commands for customizing the display, including adding annotation, right-click the data. See Data Display for more information. 7. Click Measure. Thermo Scientific Evolution 200 Series User Guide...
  • Page 236 The Run Chart tab plots the concentration of the measured component versus sample number. If Use concentration limits was selected on the Samples tab, horizontal limit lines show whether the concentrations are within the specified limits: Evolution 200 Series User Guide Thermo Scientific...

  • Page 237: Protein Biuret

    Copy to Clipboard. Related Topics Overview Application Settings Pierce 660 nm Assay Workbooks Standard Curves Data Display Protein Biuret Contents • Overview • Application Settings • Standard Curves • Making Protein Biuret Assay Measurements Thermo Scientific Evolution 200 Series User Guide...

  • Page 238: Overview

    Making Protein Biuret Assay Measurements Type Tab for Protein Biuret Click Settings in Protein Biuret to display the Type tab in the right pane. Feature Description Name Application name. Description (optional) Description of the template. Evolution 200 Series User Guide Thermo Scientific...
  • Page 239 Sloping baseline draws a line between the data points at the entered wavelengths, finds its absorbance value at the analytical wavelength, and subtracts it from the measured absorbance. Component name Component to quantify. Thermo Scientific Evolution 200 Series User Guide...
  • Page 240 4. For selected provided formulas, enter a name and concentration unit in the table. The available units depend on the Formulas & Units tab settings. Related Topics Type Tab for Protein Biuret Standards Tab for Protein Biuret Evolution 200 Series User Guide Thermo Scientific...
  • Page 241 .csv file or .tsv file, click Export Standards. Related Topics Type Tab for Protein Biuret Measurement Tab for Protein Biuret Instrument Tab for Protein Biuret Accessories Tab for Protein Biuret Thermo Scientific Evolution 200 Series User Guide...
  • Page 242 Shows the analysis wavelength(s) entered on the Measurement (Fixed only) tab. Related Topics Type Tab for Protein Biuret Measurement Tab for Protein Biuret Standards Tab for Protein Biuret Accessories Tab for Protein Biuret Samples Tab for Protein Biuret Evolution 200 Series User Guide Thermo Scientific...
  • Page 243 If available for the current unit, corrects sample concentrations using correction the entered target weight and volume: corrected concentration = measured concentration * (actual weight / target weight) * (target volume / actual volume) Thermo Scientific Evolution 200 Series User Guide...

  • Page 244: Standard Curves

    Application Settings Making Protein Biuret Assay Measurements Making Protein Biuret Assay Measurements Sample and Standards Preparation for more information. To make Protein Biuret assay measurements 1. Click Measure Protein Biuret in Protein Biuret. Evolution 200 Series User Guide Thermo Scientific...
  • Page 245 The Standard Curve(s) tab displays the resulting standard curve (or curves). Specify the standards to use for the curve (or curves) by selecting Yes or No in the Use column in the table. More: Thermo Scientific Evolution 200 Series User Guide...
  • Page 246 If Sample averaging was set to Triplicate, “D” and “T” at the end of sample names indicate the second and third measurements to be made, respectively. Evolution 200 Series User Guide Thermo Scientific...
  • Page 247 The Run Chart tab plots the concentration of the measured component versus sample number. If Use concentration limits was selected on the Samples tab, horizontal limit lines show whether the concentrations are within the specified limits: Thermo Scientific Evolution 200 Series User Guide...
  • Page 248 To copy the data on the Standard Curve(s) tab or Run Chart tab, right-click the plot and choose Copy to Clipboard. Related Topics Overview Application Settings Standard Curves Data Display Evolution 200 Series User Guide Thermo Scientific...

  • Page 249: Accessories

    Calibrated Validation Carousel (CVC) • Smart Sipper • Depolarizer • Temperature Probe • Grazing Angle Specular Reflectance • Variable Angle Transmission • Integrated Fiber Optic Module • VERSA Probe • Integrating Sphere Diffuse Reflectance Thermo Scientific Evolution 200 Series User Guide...
  • Page 250 Accessories Evolution 200 Series User Guide Thermo Scientific...

  • Page 251: Degree Near Normal Specular Reflectance

    8 Degree Near Normal Specular Reflectance For Local Control instruments: D://2616_HARRICK_8NN Spec Accy_UG.pdf For Computer Control instruments: C://2616_HARRICK_8NN Spec Accy_UG.pdf If these links do not work, see your documentation CD. Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 252 8 Degree Near Normal Specular Reflectance Evolution 200 Series User Guide Thermo Scientific...

  • Page 253: 15 Degree Specular Reflectance

    15 Degree Specular Reflectance For Local Control instruments: D://2222_PIKE_15spec Accy_UG.pdfFor Computer Control instruments: C://2222_PIKE_15spec Accy_UG.pdfIf these links do not work, see your documentation Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 254 Evolution 200 Series User Guide Thermo Scientific...
  • Page 255 20 Degree Specular Reflectance For Local Control instruments: D://2614_PIKE_20spec Accy_UG.pdfFor Computer Control instruments: C://2614_PIKE_20spec Accy_UG.pdfIf these links do not work, see your documentation Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 256 Evolution 200 Series User Guide Thermo Scientific...

  • Page 257: 30 Degree Specular Reflectance

    30 Degree Specular Reflectance For Local Control instruments: D://2223_PIKE_30spec Accy_UG.pdfFor Computer Control instruments: C://2223_PIKE_30spec Accy_UG.pdfIf these links do not work, see your documentation Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 258 Evolution 200 Series User Guide Thermo Scientific...

  • Page 259: 45 Degree Specular Reflectance

    45 Degree Specular Reflectance For Local Control instruments: D://2224_PIKE_45spec Accy_UG.pdfFor Computer Control instruments: C://2224_PIKE_45spec Accy_UG.pdfIf these links do not work, see your documentation Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 260 Evolution 200 Series User Guide Thermo Scientific...

  • Page 261: 60 Degree Specular Reflectance

    60 Degree Specular Reflectance For Local Control instruments: D://2615_PIKE_60spec Accy_UG.pdfFor Computer Control instruments: C://2615_PIKE_60spec Accy_UG.pdfIf these links do not work, see your documentation Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 262 Evolution 200 Series User Guide Thermo Scientific...

  • Page 263: Calibrated Validation Carousel (Cvc)

    Potassium dichromate Stray Light 220, 340 nm cut-off filters Sodium Iodide Potassium chloride Noise 0A and 1A Neutral Density Instrument lamp Instrument lamp filter INSIGHT software stores all instrument performance specifications and tolerances. Thermo Scientific Evolution 200 Series User Guide...

  • Page 264: Installing The Carousel On The Smart Base Unit

    Related Topics Installing Complete CVC in the Instrument CVC Test Limits Using the CVC Maintenance Installing Complete CVC in the Instrument Install the Smart Accessory as described in Installing a Smart Accessory. Contents Evolution 200 Series User Guide Thermo Scientific...

  • Page 265: Initialization

    1. In the selected application, choose Settings > Accessories > Cell Changer. 2. Click Reset. Related Topics Installing a Smart Accessory Installing the Carousel on the Smart Base Unit Loading CVC Calibration Data Thermo Scientific Evolution 200 Series User Guide...
  • Page 266 3. Locate and select the calibration file that matches the installed CVC and click OK. Related Topics Installing a Smart Accessory Installing the Carousel on the Smart Base Unit Initialization CVC Test Limits Using the CVC Maintenance Evolution 200 Series User Guide Thermo Scientific...

  • Page 267: Cvc Test Limits

    To run CVC performance verification tests 1. Click Performance Verification in Home. The available tests appear in the right pane. Thermo Scientific Evolution 200 Series User Guide...
  • Page 268 Betriebs vom Zubehör fern zu halten. CAUTELA Evitare il rischio di pizzicamento.Tenere mani e oggetti lontano dall’accessorio durante l’operazione. PRECAUCIÓN Evite riesgos de atrapamiento.Mantenga manos y objetos alejados del accesorio durante el funcionamiento. CAUTION Evolution 200 Series User Guide Thermo Scientific...

  • Page 269: Cvc Test Results

    4. Click Close. Related Topics Installing the Carousel on the Smart Base Unit Installing Complete CVC in the Instrument Loading CVC Calibration Data CVC Test Limits CVC Test Results Viewing CVC Test Reports Thermo Scientific Evolution 200 Series User Guide...
  • Page 270 To view a CVC test report 1. From the INSIGHT software Home screen, click My Data. 2. Click the PV Reports tab. The right pane shows all the performance tests run with the instrument. Evolution 200 Series User Guide Thermo Scientific...

  • Page 271: Maintenance

    Loading CVC Calibration Data CVC Test Limits Running Performance Verification Tests CVC Test Results Maintenance Maintenance Contents • Routine Maintenance • Recertification NOTICE Do not touch the exposed surface of the standards in the CVC. Thermo Scientific Evolution 200 Series User Guide...

  • Page 272: Recertification

    Before returning your accessory, contact technical support. Related Topics Installing the Carousel on the Smart Base Unit Installing Complete CVC in the Instrument Loading CVC Calibration Data Using the CVC Routine Maintenance Evolution 200 Series User Guide Thermo Scientific...

  • Page 273: Depolarizer

    Depolarizer For Local Control instruments: D://2613_HARRICK_Depolarizer Accy_UG.pdfFor Computer Control instruments: C://2613_HARRICK_Depolarizer Accy_UG.pdfIf these links do not work, see your documentation CD. Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 274 Evolution 200 Series User Guide Thermo Scientific...

  • Page 275: Grazing Angle Specular Reflectance

    For more information for Local Control instruments: D://2625_Grazing Angle Spec Accy_UG.pdfFor Computer Control instruments: C://2625_Grazing Angle Spec Accy_UG.pdfIf these links do not work, see your documentation CD. Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 276 Evolution 200 Series User Guide Thermo Scientific...

  • Page 277: Integrated Fiber Optic Module

    To avoid injury while using the accessory, do not stare at the UV beam or UV light leaving a coupled fiber optic cable. Related Topics Operating Precautions Operating Precautions Handle the accessory carefully: • Do not allow moisture to leak into the accessory. Thermo Scientific Evolution 200 Series User Guide...

  • Page 278: Installation

    Augen gegen direkte und reflektierte UV-Strahlung Gli occhiali protettivi con schermi laterali o con schermi laterali monolente N.B. sono l'unica attrezzatura che fornisce un'adeguata protezione degli occhi dalla luce UV diretta e riflessa Evolution 200 Series User Guide Thermo Scientific...
  • Page 279 AVVERTENZA Evitare il rischio di radiazioni UV.Non fissare il raggio di luce che esce dal cavo a fibre ottiche accoppiato. ADVERTENCIA Evite el riesgo de radiación ultravioleta.No mire al rayo luminoso que sale de un cable de fibra óptica acoplado. Thermo Scientific Evolution 200 Series User Guide...

  • Page 280: Attaching Cables And Probes

    2. Route the cables through the detector compartment on the instrument. 3. Attach the probe’s Light Input cable to the Light Output port on the accessory. To attach a cable, insert the SMA connector and gently tighten the ferrule by hand. Evolution 200 Series User Guide Thermo Scientific...

  • Page 281: Maintenance

    If the instrument has the AFBG option, in each application click Settings > Instrument and set Bandwidth to Fiber. Related Topics Installation Attaching Cables and Probes Aligning the Accessory Maintenance Contents • Routine Maintenance • Aligning the Accessory Thermo Scientific Evolution 200 Series User Guide...

  • Page 282: Aligning The Accessory

    4. From INSIGHT software, choose System Settings > Alignment. Alignment Tab. 5. Set the filter. Set Filter to Green (typical) or White (no filter) if the signal is low. 6. Set Bandwidth to Fiber. Evolution 200 Series User Guide Thermo Scientific...
  • Page 283 Adjust the Left/Right knob on the accessory to increase the signal further. c. Repeat steps a and b until the signal is at its maximum value. 9. Click Stop. Related Topics Installation Attaching Cables and Probes Routine Maintenance Troubleshooting Thermo Scientific Evolution 200 Series User Guide...

  • Page 284: Troubleshooting

    O-rings. See Cleaning Probes. • Damaged probe, optical fiber or Fiber Optic • If performance is still unacceptable, Coupler. contact us. See Contacting Related Topics Installation Attaching Cables and Probes Operation Maintenance Evolution 200 Series User Guide Thermo Scientific...

  • Page 285: Integrating Sphere Diffuse Reflectance

    This 60 mm Spectralon® integrating sphere accessory supports reflectance and transmittance measurements. Specular included and specular excluded measurements are supported for reflectance. Solids and solutions, in up to 50 mm pathlength cuvettes, are supported for transmittance. Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 286 Integrating Sphere Diffuse Reflectance Evolution 200 Series User Guide Thermo Scientific...

  • Page 287: Mercury Lamp

    Pour ne pas déjouer le verrouillage de sécurité, tenez les aimants et autres champs magnétiques puissants (tels que ceux produits par un spectromètre de masse ou divers instruments médicaux) éloignés de la lampe lorsque l’instrument est sous tension et que le câble de la lampe est raccordé. Thermo Scientific Evolution 200 Series User Guide...
  • Page 288 (como los producidos por un espectrómetro de masas o diversos instrumentos médicos) lejos de la lámpara mientras el instrumento esté encendido y el cable de la lámpara esté conectado. Related Topics Operating Precautions Installation and Operation Evolution 200 Series User Guide Thermo Scientific...

  • Page 289: Operating Precautions

    WARNUNG Gefährliche UV-Strahlung im Gehäuse der Quecksilberlampe ist zu vermeiden. Augen und Haut müssen vor der Strahlung geschützt werden. AVVERTENZA Evitare il rischio di radiazione UV nel vano della lampada al mercurio. Proteggere occhi e pelle dall’esposizione. Thermo Scientific Evolution 200 Series User Guide...
  • Page 290 Verification. If you have a custom button configuration, click Home, select the Classic group and then click Performance Verification. Select the following PV tests (deselect all other tests) and then click Run: • Wavelength Accuracy - Hg Lines • Wavelength Reproducibility - Hg 546 Evolution 200 Series User Guide Thermo Scientific...
  • Page 291 Samples can be analyzed only when the lamp is not in the port. To disconnect the mercury lamp Unplug the lamp cable and place the lamp in the provided case. Thermo Scientific Evolution 200 Series User Guide...
  • Page 292 Mercury Lamp Related Topics Safety Considerations Operating Precautions Evolution 200 Series User Guide Thermo Scientific...

  • Page 293: Nanocell

    NOTICE Never submerge any portion of the nanoCell in liquid. To measure samples with the nanoCell 1. Insert the nanoCell into the sample position single cell holder. Make sure one of the quartz windows is facing the light beam entering the nanoCell. Thermo Scientific Evolution 200 Series User Guide...
  • Page 294 6. Use a laboratory tissue or microfiber cloth to wipe the sample loading area and the mirror in the lid. 7. Repeat the loading and cleaning steps to measure your samples. Related Topics Using the nanoCell Cleaning the nanoCell Pathlength Correction Factors Frequently Asked Questions Evolution 200 Series User Guide Thermo Scientific...

  • Page 295: Overview

    The light that passes through the sample is coupled into a second fiber optic cable and is launched out of the nanoCell accessory. The absorbance of the solution is measured using the detector in the spectrophotometer. Thermo Scientific Evolution 200 Series User Guide...

  • Page 296: Introduction To Small Volume Measurements

    10 (1.0 mm pathlength) or 50 (0.2 mm pathlength) compared to a traditional 10 mm pathlength cuvette. The Beer Lambert law relates the measured absorbance of a solution to the concentration of that solution. The Beer Lambert equation is: Evolution 200 Series User Guide Thermo Scientific...

  • Page 297: Using The Nanocell

    The nanoCell ships configured for a z-height (distance from the bottom of the cell to the center of the light beam) of 8.5 mm. An adapter is included for 15 mm z-height instruments. The adapter simply screws on the base of the nanoCell accessory. Thermo Scientific Evolution 200 Series User Guide...
  • Page 298 Frequently Asked Questions Loading a sample To load a sample on the nanoCell, use a small volume pipette to place between 0.70 μl and 3.0 μl of solution on the sample loading window. Evolution 200 Series User Guide Thermo Scientific...
  • Page 299 Carefully place the appropriate lid on top of the sample loading area so that it sits in each of the two locator pins near the top of the nanoCell. Figure 5. (A) Locator pins on the top of the nanoCell used to position the lid. (B) Locator grooves in the lid. Thermo Scientific Evolution 200 Series User Guide...

  • Page 300: Measurements

    Some measurement errors are due to a poor baseline or zero. Related Topics Installing the nanoCell Loading a sample Cleaning the nanoCell Storing the nanoCell Pathlength Correction Factors Frequently Asked Questions Evolution 200 Series User Guide Thermo Scientific...

  • Page 301: Cleaning The Nanocell

    Storing the nanoCell Pathlength Correction Factors Frequently Asked Questions Storing the nanoCell Store the nanoCell and lids in the provided case. Related Topics Using the nanoCell Cleaning the nanoCell Pathlength Correction Factors Frequently Asked Questions Thermo Scientific Evolution 200 Series User Guide...

  • Page 302: Pathlength Correction Factors

    1.55 15.5 3,875 Warburg, O.; Christian Protein conc = [(A ) - (A 0.76 1,900 Christian, W., Where: Biochem Z. 1942, = absorption at 280 nm 310, 384-421. = absorption at 260 nm Evolution 200 Series User Guide Thermo Scientific...

  • Page 303: Frequently Asked Questions

    Yes. The quartz sample loading area of the nanoCell accessory and the mirror in the lid are the same as a traditional quartz cuvette. See Cleaning the nanoCell. Thermo Scientific Evolution 200 Series User Guide...
  • Page 304 See Cleaning the nanoCell. Related Topics Using the nanoCell Cleaning the nanoCell Storing the nanoCell Pathlength Correction Factors Evolution 200 Series User Guide Thermo Scientific...

  • Page 305: Tps-1500W Sealed Peltier Recirculator

    USB cable. Peltier recirculator Contents • General Safety Information • Product Description • Preinstallation • Installation • Operation • Maintenance • Troubleshooting Related Topics Peltier Thermostatted Single Cell Holder Thermo Scientific Evolution 200 Series User Guide...

  • Page 306: General Safety Information

    CAUTION Always disconnect the power supply before starting any work inside the instru- ment. CAUTION Take the necessary precautions when using flammable or explosive substances in this accessory. Related Topics Product Description Preinstallation Installation Operation Maintenance Troubleshooting Evolution 200 Series User Guide Thermo Scientific...

  • Page 307: Product Description

    The front panel features an LCD display with a three-button keypad. The back panel houses the main power connector with voltage selector, RS-232 and USB connectors for remote control, main power switch and connectors for transporting liquid into and out of the unit. Thermo Scientific Evolution 200 Series User Guide...

  • Page 308: Specifications

    TPS-1500W Sealed Peltier Recirculator Related Topics Specifications General Safety Information Preinstallation Installation Operation Maintenance Troubleshooting Specifications Contents Evolution 200 Series User Guide Thermo Scientific...
  • Page 309 • Temperature repeatability: typically ±1 °C (1.8 °F) • Temperature stability: typically ±0.1 °C (±0.18 °F) • Specifications valid for ambient temperatures from 15 °C to 35 °C (59 °F to 95 °F) External Control • RS-232 • USB Thermo Scientific Evolution 200 Series User Guide...
  • Page 310 • Location of use exhibits an ambient temperature between 10 °C and 35 °C (50 °F and 95 °F). • Location of use is free from dust and vapors of solvents and acids. Related Topics Front and Back Panel General Safety Information Evolution 200 Series User Guide Thermo Scientific...

  • Page 311: Preinstallation

    Type of fuse - 250 V 240 V T 1A, Slo Blo 120 V T 2A, Slo Blo Adjustment for frequency change between 50 and 60 Hz is not necessary. Voltage display window Fuse drawer tab Thermo Scientific Evolution 200 Series User Guide...

  • Page 312: Changing The Voltage Setting Or Fuse

    Voltage selector cube 8. Rotate the cube 180 degrees and then replace the cube in the drawer. The correct voltage setting should display in the window on the bottom of the fuse drawer. Evolution 200 Series User Guide Thermo Scientific...

  • Page 313: Installation

    Before starting the recirculator, familiarize yourself with the software features described in Operation. To install the recirculator 1. Position the recirculator in a suitable location. NOTICE Leave at least 5 cm (2 in) in front and back of the unit for air circulation. Thermo Scientific Evolution 200 Series User Guide...
  • Page 314 See Installing a Liquid Thermostatted Accessory. Use the provided adapters if necessary. 7. Connect the power cord to the power connector on the recirculator back panel and turn on the power switch. Evolution 200 Series User Guide Thermo Scientific...

  • Page 315: Operation

    Product Description Preinstallation Operation Maintenance Troubleshooting Operation To start the recirculator, turn on the power switch on the back panel. The display appears in one of three modes: • Stand-By mode • Normal mode Thermo Scientific Evolution 200 Series User Guide...
  • Page 316 Contents • Standby Mode • Normal Mode • Alarm Mode Standby Mode • Press and hold the Center button for one to two seconds to proceed to Normal Mode. Related Topics Normal Mode Evolution 200 Series User Guide Thermo Scientific...
  • Page 317 1. From the main Normal Mode page, press the Change button to highlight Set Temperature. The Change Set Point page appears with down and up arrows above the Left and Center buttons. 2. Use the down and up arrow buttons to change the set temperature. Thermo Scientific Evolution 200 Series User Guide...
  • Page 318 A list of diagnostic readouts appears. • Use the left and center buttons to scroll the screen up and down. • Use the right (Exit) button to return to the main Normal Mode page. Evolution 200 Series User Guide Thermo Scientific...
  • Page 319 • Use the center (Beep) button to turn off the audible alarm. • Use the right (Reset) button, if available, to reset the audible alarm. See Manual reset prealarm in the Parameters Menu to configure the Reset option to display in Alarm Mode. Thermo Scientific Evolution 200 Series User Guide...

  • Page 320: Maintenance

    Cleaning the Accessory Filling the Reservoir Preinstallation Installation Operation Troubleshooting Cleaning the Accessory To clean the accessory, use a cloth dampened with water or neutral detergent only. Do not use organic or abrasive solvents. Evolution 200 Series User Guide Thermo Scientific...

  • Page 321: Filling The Reservoir

    1. Open the vent by turning the control ring counterclockwise. Place a container under the vent opening to catch drips. Vent control ring 2. Fill the supplied bottle with distilled water. The bottle lid has a tube with a quick connect fitting. Thermo Scientific Evolution 200 Series User Guide...

  • Page 322: Acoustic Alarm

    RS-232 or USB connections. Contents • Acoustic Alarm • Water Level Failure • Other System Failures Acoustic Alarm The accessory sends an acoustic alarm when it detects a leak or if another failure occurs. Evolution 200 Series User Guide Thermo Scientific...
  • Page 323 3. Open the sample compartment and clean up any liquids that may have leaked from the accessory. 4. Tighten all connectors to and from the recirculator. 5. Fill the reservoir. See Filling the Reservoir. 6. Turn on the recirculator power switch. Thermo Scientific Evolution 200 Series User Guide...

  • Page 324: Water Level Failure

    If the failure incorrect use reoccurs, turn off the power and contact technical support. See Contacting Related Topics Acoustic Alarm Water Level Failure Preinstallation Installation Operation Maintenance Evolution 200 Series User Guide Thermo Scientific...

  • Page 325: Peltier Thermostatted Single Cell Holder

    Controller Cell holder Contents • General Safety Information • Product Description • Preinstallation • Installation • Operation • Maintenance • Troubleshooting Related Topics TPS-1500W Sealed Peltier Recirculator Thermo Scientific Evolution 200 Series User Guide...

  • Page 326: General Safety Information

    CAUTION Always disconnect the power supply before starting any work inside the instru- ment. CAUTION Take the necessary precautions when using flammable or explosive substances in this accessory. Related Topics Product Description Preinstallation Installation Operation Maintenance Troubleshooting Evolution 200 Series User Guide Thermo Scientific...

  • Page 327: Product Description

    The controller front panel features an LCD display with a three-button keypad. The rear panel houses the main power connection with voltage selector, RS-232 and USB connectors for remote control, main power switch and cell holder connector. Thermo Scientific Evolution 200 Series User Guide...

  • Page 328: Specifications

    Description of Symbols • Environmental Conditions Display/Keypad The controller has an LCD screen and 3-button keypad to access the functions and set sample temperature and stirring rate. See Operation for details. Programmable Parameters • Temperature Evolution 200 Series User Guide Thermo Scientific...
  • Page 329 • Depth: 269 mm (10.6 in) • Weight: 3 kg (8 lbs) Electrical Data • Voltage: 115/120 V or 220/240 V ±10% • Frequency: 50/60 Hz • Power: 80 VA • CE marked Thermo Scientific Evolution 200 Series User Guide...

  • Page 330: Preinstallation

    • Location of use is free from dust and vapors of solvents and acids. Related Topics Temperature Controller Front and Rear Panels General Safety Information Preinstallation Installation Operation Maintenance Troubleshooting Preinstallation Review this section before installing the accessory. Contents Evolution 200 Series User Guide Thermo Scientific...

  • Page 331: Verifying The Power Supply And Fuse

    Adjustment for frequency change between 50 and 60 Hz is not necessary. Voltage display window Fuse drawer tab Related Topics Changing the Voltage Setting or Fuse General Safety Information Preinstallation Installation Operation Maintenance Troubleshooting Thermo Scientific Evolution 200 Series User Guide...
  • Page 332 15. Turn on the main power switch on the Peltier unit back panel. If the unit does not perform normally or if the replacement fuse blows, contact technical support. Related Topics Verifying the Power Supply and Fuse General Safety Information Evolution 200 Series User Guide Thermo Scientific...

  • Page 333: Installation

    Sample Compartment Front Panel. 3. Remove the tubing access panel. See Tubing Access Panel. 4. Remove any existing cell holder or accessory. 5. Connect one end of the provided flat cable to the cell holder. Thermo Scientific Evolution 200 Series User Guide...
  • Page 334 9. Use appropriate tubing and the provided adapters to connect the two fluid ports on the cell holder to a water pump or fluid recirculator. See Installing a Liquid Thermostatted Accessory. Ensure the tubing does not obstruct the light beam. Evolution 200 Series User Guide Thermo Scientific...

  • Page 335: Operation

    14. Set the software parameters to configure the temperature controller for operation. See Operation. Related Topics General Safety Information Product Description Preinstallation Operation Maintenance Troubleshooting Operation Contents • Setting the Target Temperature and Stir Speed • Measuring Samples Thermo Scientific Evolution 200 Series User Guide...

  • Page 336: Setting The Target Temperature And Stir Speed

    If a Peltier cell holder or changer is used, defines a setpoint temperature for the cell block and a stir speed if desired. The available settings depend on the specifications for the temperature-controlled accessory. Evolution 200 Series User Guide Thermo Scientific...
  • Page 337 The probe channel, current temperature and target range appear during the measurement. • For a temperature-controlled accessory. To start the measurement when the cell block reaches the target temperature, set Start on to Block temperature. Thermo Scientific Evolution 200 Series User Guide...
  • Page 338 To display the temperature status monitor 1. Click the Configure button above the data display for the selected application. See Instrument Status Monitors for more information. A dialog box lists the available status monitors. Evolution 200 Series User Guide Thermo Scientific...
  • Page 339 After the controller power is turned on, the display appears in one of three modes: Stand-By, Normal, or Alarm. The image below indicates Normal mode. Stirring Actual block Heating/cooling speed temperature power scale Block set temperature Thermo Scientific Evolution 200 Series User Guide...
  • Page 340 Scroll Exit Diagnostic Diagnostic Values Down Exit Changing the set temperature 1. Highlight Set Temperature. The Change Set Point page is displayed with down and up arrows above the Left and Center buttons. Evolution 200 Series User Guide Thermo Scientific...
  • Page 341 • Use the Left and Center buttons to scroll the screen up and down. • Use the Right button to exit the screen and return to the Main Menu. Description Measure unit Cell temperature °C Radiator temperature °C Thermo Scientific Evolution 200 Series User Guide...

  • Page 342: Routine Maintenance

    Related Topics Cleaning the Accessory Preinstallation Installation Operation Troubleshooting Cleaning the Accessory To clean the accessory, use a cloth dampened with water or neutral detergent only. Do not use organic or abrasive solvents. Evolution 200 Series User Guide Thermo Scientific...

  • Page 343: Troubleshooting

    If malfunction or the failure reoccurs, turn off the power and incorrect use contact technical support. See Contacting Related Topics General Safety Information Product Description Preinstallation Installation Operation Maintenance Troubleshooting Thermo Scientific Evolution 200 Series User Guide...
  • Page 344 Peltier Thermostatted Single Cell Holder Evolution 200 Series User Guide Thermo Scientific...

  • Page 345: Probe Handling And Maintenance

    • Do not drop the probe or knock it against a hard surface. • Do not subject the probe to rapid or excessive changes in temperature. • Wipe off spilled chemicals immediately. Thermo Scientific Evolution 200 Series User Guide...

  • Page 346: Installation

    AVERTISSEMENT Évitez l'exposition au rayonnements UV. Ne regardez jamais le faisceau de lumière qui sort d’une fibre optique couplé. WARNUNG Gefahren durch UV-Strahlung sind zu vermeiden. Nicht in den Strahl eines gekoppelten Glasfaserkabels blicken. Evolution 200 Series User Guide Thermo Scientific...

  • Page 347: Operation

    If the probe has a remote start cable, plug the connector into the Trigger port on the spectrophotometer. See Connectors. Related Topics Safety Considerations Operating Precautions Operation Maintenance Operation Follow the probe manufacturer’s recommendations for compatible samples and operating procedures. Related Topics Safety Considerations Operating Precautions Thermo Scientific Evolution 200 Series User Guide...

  • Page 348: Maintenance

    Follow these instructions to clean a probe purchased from us. For recommended agents and procedures to clean other probes, refer to the manufacturer’s instructions. NOTICE • Do not use an ultrasonic bath to clean fiber optic probes. • Use only recommended cleaning agents. Evolution 200 Series User Guide Thermo Scientific...
  • Page 349 NOTICE Do not soak the lens paper or swab with the cleaning agent. This may leave pools of liquid that do not dry properly. To remove stubborn materials, use a small amount of acetone. Related Topics Safety Considerations Operating Precautions Installation Operation Routine Maintenance Troubleshooting Thermo Scientific Evolution 200 Series User Guide...
  • Page 350 Probe Handling and Maintenance Evolution 200 Series User Guide Thermo Scientific...

  • Page 351: Rapid Mix Accessory

    Rapid Mix Accessory For Local Control instruments: D://2606_APPL PHOTOPHYSICS_Stop Flow Accy_UG.pdfFor Computer Control instruments: C://2606_APPL PHOTOPHYSICS_Stop Flow Accy_UG.pdfIf these links do not work, see your documentation CD. Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 352 Evolution 200 Series User Guide Thermo Scientific...

  • Page 353: Reference Cell Holders

    Installing a Reference Cell Holder Sample Position Cell Holders Cylindrical Reference Cell Holder Cylindrical Reference Cell Holder This holder fits cylindrical cells with the following pathlengths: 10 mm, 20 mm, 50 mm and 100 mm. Thermo Scientific Evolution 200 Series User Guide...
  • Page 354 Reference Cell Holders Related Topics Installing a Reference Cell Holder Sample Position Cell Holders Rectangular Reference Cell Holder Evolution 200 Series User Guide Thermo Scientific...

  • Page 355: Sample Position Cell Holders

    Use the cell lifter to raise the cell for easy removal. This cell holder allows fine adjustment of the vertical and horizontal position of the cell. This feature is useful for aligning reduced volume and microcuvettes with the light beam. Thermo Scientific Evolution 200 Series User Guide...

  • Page 356: Water Thermostatted 10 Mm Rectangular Cell Holder

    5 °C and 95 °C (see Installing a Liquid Thermostatted Accessory). Two ports are provided to connect the cell holder to a recirculating liquid temperature control device. The connectors accept 1/4-inch internal diameter tubing. Evolution 200 Series User Guide Thermo Scientific...

  • Page 357: Cell Holder For 15 Mm Beam Height Cells

    Cell Holder for 15 mm Beam Height Cells This cell holder accommodates 10 mm pathlength cells designed for instruments with a 15 mm z-height. The holder positions a 15 mm z-height cell properly for use with your spectrophotometer. Thermo Scientific Evolution 200 Series User Guide...

  • Page 358: Cylindrical Cell Holder

    Variable Pathlength Cell Holder Slide Cylindrical Cell Holder This accessory accommodates cylindrical cells with a 22 mm external diameter and a pathlength of up to 100 mm. A flexible clip holds the cell in place. Evolution 200 Series User Guide Thermo Scientific...

  • Page 359: Solid Sample Holders

    Height Width Thickness 50.8 mm (2 inches) 76.2 mm (3 inches) 2 mm (1/16 inch) The fixed position Sample Slide Holder offers two positioning options, left (detector) side and right (monochromator) side. Thermo Scientific Evolution 200 Series User Guide...
  • Page 360 Universal Sample Holder Slide Rotatable Test Tube and Cell Holder Sample Holder for 1-Inch Square Cells Sample Holder for Small Test Tubes and Vials Variable Pathlength Rectangular Cell Holder Variable Pathlength Cell Holder Slide Evolution 200 Series User Guide Thermo Scientific...

  • Page 361: Universal Sample Holder Slide

    10 mm pathlength cell. The base can be rotated to properly position the cell or test tube at the center of the sample compartment. The clip can also be used to secure filters, thin films and other solid samples in the light beam. Thermo Scientific Evolution 200 Series User Guide...

  • Page 362: Sample Holder For 1-Inch Square Cells

    Sample Holder for Small Test Tubes and Vials Variable Pathlength Rectangular Cell Holder Variable Pathlength Cell Holder Slide Sample Holder for 1-Inch Square Cells This sample holder accommodates cells that are 1-inch square such as Hach® cells and AccuVac® vials. Evolution 200 Series User Guide Thermo Scientific...

  • Page 363: Sample Holder For Small Test Tubes And Vials

    This accessory can be used with test tubes and vials from 10 mm to 16 mm in diameter and up to 100 mm high. NOTICE Exceeding the recommended diameter will cause the retaining clip to block the light beam. Thermo Scientific Evolution 200 Series User Guide...

  • Page 364: Variable Pathlength Rectangular Cell Holder

    This accessory accommodates rectangular cells with pathlengths ranging from 1 mm to 100 mm. Use the knob to adjust the accessory to accommodate your cell. NOTICE Overtightening the accessory may damage or break the cell. Evolution 200 Series User Guide Thermo Scientific...

  • Page 365: Variable Pathlength Cell Holder Slide

    Sample Holder for Small Test Tubes and Vials Variable Pathlength Cell Holder Slide Variable Pathlength Cell Holder Slide This accessory accommodates rectangular cells with pathlengths ranging from 1 mm to 50 mm and requires a variable position Solid Sample Holder). Thermo Scientific Evolution 200 Series User Guide...
  • Page 366 Cylindrical Cell Holder Solid Sample Holders Universal Sample Holder Slide Rotatable Test Tube and Cell Holder Sample Holder for 1-Inch Square Cells Sample Holder for Small Test Tubes and Vials Variable Pathlength Rectangular Cell Holder Evolution 200 Series User Guide Thermo Scientific...
  • Page 367 8-cell holder Smart Accessory base Contents • Safety Considerations • Operating Precautions • Installation • Operation • Maintenance • Troubleshooting Related Topics SPE-8W Smart Peltier Linear Cell Changer Smart Rotary Cell Changer Accessories Thermo Scientific Evolution 200 Series User Guide...

  • Page 368: Smart Linear 8-Cell Changer

    • Do not drop the accessory. • Protect the accessory from mechanical shock. Related Topics Safety Considerations Installation Operation Maintenance Troubleshooting Installation Contents • Installing a Smart Cell Changer • Replacing the Cell Holder Evolution 200 Series User Guide Thermo Scientific...
  • Page 369 à l’écart de l’accessoire pendant l’initialisation. VORSICHT Quetschungen sind zu vermeiden. Hände und Objekte sind während der Initialisierung vom Zubehör fern zu halten. CAUTELA Evitare il rischio di pizzicamento. Tenere mani e oggetti lontano dall’accessorio durante l’inizializzazione. Thermo Scientific Evolution 200 Series User Guide...

  • Page 370: Calibrating The Cell Changer

    Replacing the Cell Holder Calibrating the Cell Changer Cell Changer Settings Displaying the Cell Changer Status Monitor Installation Replacing the Cell Holder To remove the cell holder 1. Loosen the two locking screws. Evolution 200 Series User Guide Thermo Scientific...
  • Page 371 Calibrating the Cell Changer • Initializing the Cell Changer • Cell Changer Settings • Measuring Samples with the Cell Changer • Displaying the Cell Changer Status Monitor Related Topics Safety Considerations Operating Precautions Thermo Scientific Evolution 200 Series User Guide...
  • Page 372 A status box shows several intensity values collected on each side of the expected best location for each cell. The average of the highest readings is stored for each cell. A message box appears when calibration is completed. 3. Click OK. Evolution 200 Series User Guide Thermo Scientific...
  • Page 373 PRECAUCIÓN Evite riesgos de atrapamiento. Mantenga manos y objetos lejos del alcance del accesorio durante la inicialización. To initialize a cell changer 1. In the selected application, choose Settings > Accessories > Cell Changer. 2. Click Reset. Thermo Scientific Evolution 200 Series User Guide...
  • Page 374 Initializes the cell changer and moves cell one into the light beam. Related Topics Calibrating the Cell Changer Initializing the Cell Changer Measuring Samples with the Cell Changer Displaying the Cell Changer Status Monitor Operation Evolution 200 Series User Guide Thermo Scientific...
  • Page 375 7. To measure a blank without using the sample list, click Measure Fixed and then click Zero (or Measure Scan and then Baseline). The Loading Guide shows one entry for the blank measurement. Thermo Scientific Evolution 200 Series User Guide...
  • Page 376 12. Place the cuvettes in the correct cell changer positions. Note If the application requires a blank and it was not measured in step 4, the first cell changer position will be measured as the blank. Evolution 200 Series User Guide Thermo Scientific...
  • Page 377 Note The cell changer option appears in the dialog box only when a supported cell changer accessory is installed. 2. Select Cell changer status and click OK. A status monitor showing the current cell changer position appears above the data display. Thermo Scientific Evolution 200 Series User Guide...

  • Page 378: Routine Maintenance

    Clean the accessory regularly using approved methods (wipe off spilled chemicals immediately). See Cleaning the Accessory. Related Topics Cleaning the Accessory Maintenance Troubleshooting Cleaning the Accessory NOTICE Do not allow moisture to leak into the accessory interior. Evolution 200 Series User Guide Thermo Scientific...

  • Page 379: Troubleshooting

    Calibrate and then initialize the cell changer. values are aligned properly See Calibrating the Cell Changer and inconsistent or Initializing the Cell Changer. higher than expected Related Topics Safety Considerations Operating Precautions Installation Operation Maintenance Thermo Scientific Evolution 200 Series User Guide...
  • Page 380 Smart Linear 8-Cell Changer Evolution 200 Series User Guide Thermo Scientific...

  • Page 381: Spe-8W Smart Peltier Linear Cell Changer

    INSIGHT software. The Peltier controller may also be used to agitate the sample by placing a magnetic stirrer in the cuvette and selecting the stir speed. Peltier temperature controller Linear cell changer Contents Thermo Scientific Evolution 200 Series User Guide...

  • Page 382: General Safety Information

    • The equipment installation is carried out in accordance with local requirements for responsibility and warranty. CAUTION Always disconnect the power supply before starting any work inside the instru- ment. CAUTION Take the necessary precautions when using flammable or explosive substances in this accessory. Evolution 200 Series User Guide Thermo Scientific...

  • Page 383: Product Description

    The accessory provides thermoelectric heating and cooling through the cell block. Contents • System Connections • External Connections • Temperature Controller Front and Rear Panel • Specifications Related Topics General Safety Information Preinstallation Installation Operation Maintenance Troubleshooting Thermo Scientific Evolution 200 Series User Guide...
  • Page 384 Communications are performed manually or via remote control through the RS-232 or USB connector. Related Topics System Connections Temperature Controller Front and Rear Panel Specifications Temperature Controller Front and Rear Panel The controller front panel features an LCD display with a three-button keypad. Evolution 200 Series User Guide Thermo Scientific...
  • Page 385 The rear panel houses the main power connection with voltage selector, RS-232 and USB connectors for remote control, main power switch and cell holder connector. Related Topics System Connections External Connections Specifications Specifications This section describes the specifications for the Peltier temperature controller. Contents Thermo Scientific Evolution 200 Series User Guide...
  • Page 386 • Temperature repeatability: typically ±0.1 °C (±0.18 °F) • Temperature stability: typically ±0.1 °C (±0.18 °F) • Specifications valid for ambient temperatures from 15 °C to 35 °C (59 °F to 95 °F) Evolution 200 Series User Guide Thermo Scientific...
  • Page 387 • Location of use exhibits an ambient temperature between 10 °C and 35 °C (50 °F and 95 °F). • Location of use is free from dust and vapors of solvents and acids. Thermo Scientific Evolution 200 Series User Guide...

  • Page 388: Preinstallation

    Power Supply Type of fuse - 250 V 240 V T 1A, Slo Blo 120 V T 2A, Slo Blo Adjustment for frequency change between 50 and 60 Hz is not necessary. Evolution 200 Series User Guide Thermo Scientific...
  • Page 389 5. Use a small screwdriver to pry the fuse drawer out of the fuse compartment by pulling the tab up and away from the drawer. Fuse drawer 6. To change the voltage setting, remove the voltage selector cube from the drawer and carefully remove the fuse from the cube. Thermo Scientific Evolution 200 Series User Guide...

  • Page 390: Installation

    Before starting the accessory, familiarize yourself with the software features described in Operation. To agitate the sample during the measurement, place a magnetic stirrer in the cuvette and use the software to set the stirring speed. Evolution 200 Series User Guide Thermo Scientific...
  • Page 391 ATTENTION Évitez tout risque de pincement. Gardez vos mains, ainsi que tout objet, à l’écart de l’accessoire pendant l’initialisation. VORSICHT Quetschungen sind zu vermeiden. Hände und Objekte sind während der Initialisierung vom Zubehör fern zu halten. Thermo Scientific Evolution 200 Series User Guide...
  • Page 392 Water ports 10. Replace the sample compartment front panel. See Sample Compartment Front Panel. 11. Close the sample compartment cover. 12. Turn on the power switch on the controller back panel. Evolution 200 Series User Guide Thermo Scientific...

  • Page 393: Operation

    Initializing the Cell Changer • Setting the Target Temperature and Stir Speed • Configuring the Cell Changer • Measuring Samples with the Cell Changer Related Topics General Safety Information Product Description Preinstallation Installation Maintenance Troubleshooting Thermo Scientific Evolution 200 Series User Guide...
  • Page 394 The average of the highest readings is stored for each cell. A message box appears when calibration is completed. 3. Click OK. Related Topics Initializing the Cell Changer Setting the Target Temperature and Stir Speed Configuring the Cell Changer Evolution 200 Series User Guide Thermo Scientific...
  • Page 395 To initialize a cell changer 1. In the selected application, choose Settings > Accessories > Cell Changer. 2. Click Reset. Related Topics Calibrating the Cell Changer Configuring the Cell Changer Operation Thermo Scientific Evolution 200 Series User Guide...

  • Page 396: Setting The Target Temperature And Stir Speed

    In the selected application, use Settings > Accessories > Temperature to display the available settings for a temperature probe or controller. The Temperature features appear only when the driver for a temperature probe or controller is installed. Evolution 200 Series User Guide Thermo Scientific...
  • Page 397 Probe temperature and then select the Probe channel. The number of channels in the list box will match the available ports on the temperature probe interface box. The probe channel, current temperature and target range appear during the measurement. Thermo Scientific Evolution 200 Series User Guide...
  • Page 398 This disables the Start button in the status dialog box. The measurement starts automatically when the temperature remains in the target range during the entire equilibration period. Related Topics Temperature and Stirrer Settings Evolution 200 Series User Guide Thermo Scientific...
  • Page 399 • Standby Mode • Normal Mode Startup Mode After the controller power is turned on, the display appears in one of three modes: Stand-By, Normal, or Alarm. The image below indicates Normal mode. Thermo Scientific Evolution 200 Series User Guide...
  • Page 400 • Press and hold the Center button for one to two seconds to return to Stand-By Mode. • From the Startup page in Normal Mode, use the Change button to move about the screen. Evolution 200 Series User Guide Thermo Scientific...
  • Page 401 • Use the Right button to exit the screen and return to the Main Menu. Table 9. User parameters and button functions Number Description Default value P-01 Temperature units °C P-02 Auto start P-03 Display contrast P-04 Beeper P-05 Manual reset pre-alarm Thermo Scientific Evolution 200 Series User Guide...
  • Page 402 Configuring the Cell Changer Contents • Cell Changer Settings • Displaying the Cell Changer Status Monitor Related Topics Calibrating the Cell Changer Initializing the Cell Changer Setting the Target Temperature and Stir Speed Operation Evolution 200 Series User Guide Thermo Scientific...
  • Page 403 Note The cell changer option appears in the dialog box only when a supported cell changer accessory is installed. 2. Select Cell changer status and click OK. A status monitor showing the current cell changer position appears above the data display. Thermo Scientific Evolution 200 Series User Guide...

  • Page 404: Measuring Samples With The Cell Changer

    5. Under Available Cells, select the check boxes for the cells that will be used in the cell changer. To skip a cell changer position, deselect its check box. Note Position 1 can not be deselected. Evolution 200 Series User Guide Thermo Scientific...
  • Page 405 10. Use Load Samples to import the sample list or enter sample names and descriptions if desired. See Settings > Samples to create the list ahead of time. 11. Click OK. The loading guide shows the measurement sequence for the cell changer as defined in the sample list. Thermo Scientific Evolution 200 Series User Guide...

  • Page 406: Maintenance

    16. Continue as above until all entries in the sample list have been measured. Related Topics Calibrating the Cell Changer Initializing the Cell Changer Setting the Target Temperature and Stir Speed Configuring the Cell Changer Operation Maintenance Contents • Routine Maintenance • Cleaning the Accessory Evolution 200 Series User Guide Thermo Scientific...
  • Page 407 Cleaning the Accessory Maintenance Troubleshooting Cleaning the Accessory To clean the accessory, use a cloth damped with water or neutral detergent only. Do not use organic or abrasive solvents. Related Topics Routine Maintenance Maintenance Troubleshooting Thermo Scientific Evolution 200 Series User Guide...

  • Page 408: Troubleshooting

    If the incorrect use failure reoccurs, turn off the power and contact technical support. See Contacting Related Topics General Safety Information Product Description Preinstallation Installation Operation Maintenance Evolution 200 Series User Guide Thermo Scientific...

  • Page 409: Smart Rotary Cell Changer

    Standard cuvette Cell holder Smart Accessory base Contents • Safety Considerations • Operating Precautions • Installation • Operation • Maintenance Related Topics Smart Linear 8-Cell Changer Accessories Thermo Scientific Evolution 200 Series User Guide...

  • Page 410: Safety Considerations

    • Do not drop the accessory. • Protect the accessory from mechanical shock. Related Topics Safety Considerations Installation Operation Maintenance Installation Contents • Installing a Smart Cell Changer • Replacing the Cell Holder Related Topics Safety Considerations Evolution 200 Series User Guide Thermo Scientific...
  • Page 411 CAUTELA Evitare il rischio di pizzicamento. Tenere mani e oggetti lontano dall’accessorio durante l’inizializzazione. PRECAUCIÓN Evite riesgos de atrapamiento. Mantenga manos y objetos lejos del alcance del accesorio durante la inicialización. 5. Replace the sample compartment front panel. Thermo Scientific Evolution 200 Series User Guide...
  • Page 412 1. Line up the tab on the underside of the cell holder with the notch on the motor shaft. Tab on underside of cell holder Notch on motor shaft of Smart base unit 2. Place the cell holder on the shaft. Evolution 200 Series User Guide Thermo Scientific...

  • Page 413: Operation

    Smart base unit is installed. Initializing verifies the cell position and resets the cell holder to position 1. CAUTION Avoid pinch hazard. Keep hands and objects clear of the accessory during initialization. Thermo Scientific Evolution 200 Series User Guide...
  • Page 414 In the selected application, use Settings > Accessories > Cell Changer to display the available settings for a cell changer. The Cell Changer features appear only when a cell changer accessory is installed. Evolution 200 Series User Guide Thermo Scientific...
  • Page 415 Remarque Évitez les risques de pincement. Éloignez les mains et les objets de l’accessoire pendant son fonctionnement. Anmerkung Quetschungen sind zu vermeiden. Hände und Objekte sind während des Betriebs vom Zubehör fern zu halten. Thermo Scientific Evolution 200 Series User Guide...
  • Page 416 The Loading Guide shows one entry for the blank measurement. Place the approproate material (or nothing) in position 1 of the cell changer and click OK to perform the measurement. 8. Click Measure. A dialog box shows the current sample list. Evolution 200 Series User Guide Thermo Scientific...
  • Page 417 Guide reappears after the last cuvette is measured. 14. If the loading guide appears, remove all cuvettes from the cell changer and fill it with the next set of samples as defined in the loading guide. 15. Click OK. Thermo Scientific Evolution 200 Series User Guide...
  • Page 418 Status Monitors” for more information. A dialog box appears. Note The cell changer option appears in the dialog box only when a supported cell changer accessory is installed. 2. Select Cell changer status and click OK. Evolution 200 Series User Guide Thermo Scientific...

  • Page 419: Maintenance

    Clean the accessory regularly using approved methods (wipe off spilled chemicals immediately). See Cleaning the Accessory. Related Topics Cleaning the Accessory Maintenance Cleaning the Accessory NOTICE Do not allow moisture to leak into the accessory interior. Thermo Scientific Evolution 200 Series User Guide...
  • Page 420 Accessory. 2. Use a cloth dampened with plain water to wipe the surface of the Smart Accessory base and cell holder. 3. Dry the surface with another cloth. Related Topics Routine Maintenance Maintenance Evolution 200 Series User Guide Thermo Scientific...

  • Page 421: Smart Sipper

    • Maintenance • Specifications • Troubleshooting Related Topics Accessories Safety Considerations To avoid injury, remove the Smart Sipper from the spectrophotometer or turn off the spectrophotometer main power switch before installing pump tubing. Thermo Scientific Evolution 200 Series User Guide...
  • Page 422 Para evitar lesiones, extraiga el aspirador de muestras Smart del espectrofotómetro o ponga el interruptor de alimentación en la posición de apagado antes de instalar los tubos de la bomba. Related Topics Operating Precautions Installation Calibration Operation Maintenance Specifications Troubleshooting Evolution 200 Series User Guide Thermo Scientific...

  • Page 423: Operating Precautions

    Related Topics Safety Considerations Installation Calibration Operation Maintenance Specifications Troubleshooting Installation Contents • Installing Pump Tubing • Installing the Smart Sipper • Installing a Flow Cell Related Topics Safety Considerations Operating Precautions Calibration Operation Thermo Scientific Evolution 200 Series User Guide...
  • Page 424 2. Cut an 18 cm length of pump tubing. 3. Taking care not to stretch, slacken or twist the tubing, feed it counterclockwise around the track while manually rotating the pump head. Evolution 200 Series User Guide Thermo Scientific...
  • Page 425 Related Topics Installing the Smart Sipper Installing a Flow Cell Installation Installing the Smart Sipper It is not necessary to power off the instrument when installing or removing a Smart Accessory. Thermo Scientific Evolution 200 Series User Guide...
  • Page 426 Initialization reads information about the accessory and reset the pump motors. 6. Install the Sipper sample compartment front panel. Align the tab on the bottom edge with the slot in the instrument cover and press the panel in place. Evolution 200 Series User Guide Thermo Scientific...
  • Page 427 The provided cell holder accommodates 12.5 mm x 12.5 mm flow cells. Other compatible holders and flow cells are available. All compatible cell holders have a central screw that mounts to the Smart Sipper base. Thermo Scientific Evolution 200 Series User Guide...
  • Page 428 Flanged tube Screw connector Rubber washer Plastic O-ring Flange b. Screw the cap into the Out port on the flow cell. c. Attach the open end of the tubing to the Pump In connector. Evolution 200 Series User Guide Thermo Scientific...

  • Page 429: Calibration

    Install the pump tubing, Smart Sipper and flow cell, including all tubing connections (see Installation) before calibration. CAUTION Avoid pinch hazard. Keep hands and objects clear of the accessory during oper- ation. Languages Thermo Scientific Evolution 200 Series User Guide...
  • Page 430 4. Enter the volume to be drawn for the calibration test in the Sip Volume box (typically 10 ml). 5. To display a prompt before each test, select Prompt. To sip automatically after a period of time, select Count down and enter the time. Evolution 200 Series User Guide Thermo Scientific...
  • Page 431 Enter this value in the Actual box and click Next. 11. Repeat steps 7 through 10 until the Actual volume matches the Expected volume. 12. Click Done. Related Topics Safety Considerations Operating Precautions Installation Operation Maintenance Specifications Troubleshooting Thermo Scientific Evolution 200 Series User Guide...

  • Page 432: Operation

    Enter 0 to skip this step. Rinse duration For automated sipper workflows that include a “rinse” step, specifies how long to draw the rinse solution through the system. Enter 0 to skip this step. Evolution 200 Series User Guide Thermo Scientific...
  • Page 433 Toggles between Pump On and Pump Off. Pump On causes the pump to draw continuously through the inlet tube; Pump Off causes it to stop. Related Topics Safety Considerations Operating Precautions Installation Calibration Maintenance Thermo Scientific Evolution 200 Series User Guide...

  • Page 434: Maintenance

    (wipe off spilled chemicals immediately). Related Topics Cleaning the Accessory Replacing Pump Tubing Cleaning Flow Cells Replacing Flow Cell Tubing Maintenance Cleaning the Accessory NOTICE Do not allow moisture to leak into the accessory interior. Evolution 200 Series User Guide Thermo Scientific...
  • Page 435 At least once a week, remove the flow cell from the accessory and soak it in a solution of Decon™ detergent (or equivalent), diluted according to the manufacturer’s instructions. When finished, reconnect the cell and flush it thoroughly with laboratory grade purified water. Thermo Scientific Evolution 200 Series User Guide...

  • Page 436: Specifications

    Cleaning the Accessory Replacing Pump Tubing Cleaning Flow Cells Maintenance Specifications Contents • Pump Tubing Chemical Compatibility • Pump Tubing Specifications • Pump Specifications Related Topics Safety Considerations Operating Precautions Installation Calibration Operation Maintenance Evolution 200 Series User Guide Thermo Scientific...
  • Page 437 — Acetone — Benzyl alcohol Acetyl bromide Bleaching liquors — Acetyl chloride Boric acid Bromine Alcohols Butane Aliphatic hydrocarbon — Butyl acetate Aluminum chloride — Butyl alcohol — Aluminum sulfate Butyric acid — Thermo Scientific Evolution 200 Series User Guide...
  • Page 438 Ethers — Hydrogen peroxide (conc) — Ethyl acetate Hydrogen sulfide Ethyl alcohol — Hydrochlorous acid Ethyl bromide Iodine solutions Ethyl chloride Kerosene Ethylamine Ketones Ethylene dichloride Lacquer solvents Ethylene glycol Lactic acid — Evolution 200 Series User Guide Thermo Scientific...
  • Page 439 Oleic acid — Sodium hypochlorite (<5%) Oxalic acid — Sodium hypochlorite (>5%) Perchloric acid Sodium sulfide Perchloroethylene Sodium sulfite Phenol Steam (up to 40psi) Phosphoric acid — Stearic acid — Phthalic acid Sulfur chloride Thermo Scientific Evolution 200 Series User Guide...
  • Page 440 Related Topics Pump Tubing Specifications Pump Specifications Specifications Pump Tubing Specifications Note Tubing that is too tight will cause excessive pump strain and wear. Tubing that is too loose will decrease pumping efficiency. Evolution 200 Series User Guide Thermo Scientific...
  • Page 441 Variable: 0.0 to 999.0 Auto function Purge, Return, Off Setting time (seconds) Variable: 0.0 to 999.0 Number of rinses Variable: 0 to 4 Related Topics Pump Tubing Chemical Compatibility Pump Tubing Specifications Specifications Thermo Scientific Evolution 200 Series User Guide...

  • Page 442: Troubleshooting

    (occurs frequently with biological materials), remove the cell and soak it in a recommended detergent. See Cleaning Flow Cells. When finished, reconnect the cell and flush it thoroughly with laboratory grade purified water. Evolution 200 Series User Guide Thermo Scientific...
  • Page 443 Smart Sipper Related Topics Safety Considerations Operating Precautions Installation Calibration Operation Maintenance Specifications Thermo Scientific Evolution 200 Series User Guide...
  • Page 444 Smart Sipper Evolution 200 Series User Guide Thermo Scientific...

  • Page 445: Temperature Probe

    Handle the accessory carefully: • Do not allow moisture to leak into the accessory. • Allow liquid to contact only the narrow measurement tip; do not submerge the probe. • Wipe off spilled chemicals immediately. Thermo Scientific Evolution 200 Series User Guide...

  • Page 446: Installation

    4. Install a cuvette in the cell holder or changer. 5. Insert the probe into an appropriate stopper (round or square, depending on the selected cuvette). NOTICE Do not force the probe into the stopper. Evolution 200 Series User Guide Thermo Scientific...

  • Page 447: Operation

    (move the changer through all positions). 10. Set the software parameters to display the probe status monitor and record sample temperature. See Operation. Related Topics Operating Precautions Operation Maintenance Troubleshooting Operation Contents Thermo Scientific Evolution 200 Series User Guide...

  • Page 448: Temperature Settings

    8 with up to eight cell changer positions. Useful only when using an automatic cell changer with multiple temperature probes. Deselect this to always record the temperature of probe 1. Related Topics Configuring a Target Temperature Displaying a Temperature Status Monitor Evolution 200 Series User Guide Thermo Scientific...
  • Page 449 The Start button is disabled since the measurement is configured to start automatically at the target temperature. 3. To allow time for equilibration, select Wait for equilibration and enter the duration. The remaining equilibration time is displayed during the measurement. Thermo Scientific Evolution 200 Series User Guide...
  • Page 450 Note Temperature options appear only when a software driver for the temperature probe is installed. 2. Select the Temperature status option that matches the probe channel and click OK. The status monitor appears above the data display. Here is an example. Evolution 200 Series User Guide Thermo Scientific...

  • Page 451: Maintenance

    Enter a name in the Description box, select the Channel and click OK. Related Topics Instrument Status Monitors Temperature Settings Configuring a Target Temperature Operation Maintenance Contents • Routine Maintenance • Cleaning Temperature Probes • Replacing Stopper O-Rings Related Topics Operating Precautions Installation Operation Troubleshooting Thermo Scientific Evolution 200 Series User Guide...
  • Page 452 To replace the internal O-ring (round or square stopper) 1. Insert the 4 mm screw several turns into the stopper base, and use it to pull the liner out of the base (leave the screw in the liner). Evolution 200 Series User Guide Thermo Scientific...
  • Page 453 1. Use tweezers or a pointed tool to remove the gaskets. 2. Stretch each new gasket onto the stopper and press it into the groove. Gaskets Related Topics Routine Maintenance Cleaning Temperature Probes Maintenance Thermo Scientific Evolution 200 Series User Guide...

  • Page 454: Troubleshooting

    Tolerance setting temperature and the in the software to account for ambient temperature. the temperature differential between the heated components. Related Topics Operating Precautions Installation Operation Maintenance Evolution 200 Series User Guide Thermo Scientific...

  • Page 455: Variable Angle Transmission

    Variable Angle Transmission For Local Control instruments: D://2612_HARRICK_Var Angle Trans Accy_UG.pdfFor Computer Control instruments: C://2612_HARRICK_Var Angle Trans Accy_UG.pdfIf these links do not work, see your documentation CD. Related Topics Installing a Manual Accessory Accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 456 Evolution 200 Series User Guide Thermo Scientific...

  • Page 457: Versa Probe

    • Do not allow hard materials (glass, metal, ceramic, etc.) to contact the probe’s optical surfaces. • Do not drop the probe or knock it against a hard surface. • Do not subject the probe to rapid or excessive changes in temperature. Thermo Scientific Evolution 200 Series User Guide...

  • Page 458: Fiber Optic Cables

    Routine Maintenance Installation Contents • Installing the VERSA Probe • Installing Tips Related Topics Operating Precautions Operation Routine Maintenance Installing the VERSA Probe Install the Integrated Fiber Optic Module before installing a probe. Evolution 200 Series User Guide Thermo Scientific...
  • Page 459 • Overtightening or using tools to tighten the connectors. If the probe has a remote start cable, plug the connector into the Trigger port on the spectrophotometer. See Connectors. Related Topics Installing Tips Thermo Scientific Evolution 200 Series User Guide...

  • Page 460: Operation

    Installation Operation Install a tip before using the probe. See Installing Tips. When inserting the probe into a solution, shake the probe gently to release any air bubbles trapped in the probe tip. Evolution 200 Series User Guide Thermo Scientific...
  • Page 461 ADVERTENCIA Evite el riesgo de radiación ultravioleta.No mire al rayo luminoso que sale de un cable de fibra óptica acoplado. WARNING To use the VERSA probe 1. Insert the probe into the baseline solution and collect a baseline or zero measurement. Thermo Scientific Evolution 200 Series User Guide...
  • Page 462 2. When finished collecting a baseline, dry the tip and insert the probe into the sample. 3. When finished measuring a sample, discard the tip or clean it before making additional measurements. Related Topics Operating Precautions Installation Routine Maintenance Evolution 200 Series User Guide Thermo Scientific...

  • Page 463: Routine Maintenance

    Follow these guidelines: • Clean the probe immediately after each measurement and before storing it. See Cleaning Probes. • Follow the manufacturer’s recommendations for probe maintenance. Related Topics Cleaning Probes Troubleshooting Operating Precautions Installation Operation Thermo Scientific Evolution 200 Series User Guide...
  • Page 464 VERSA Probe Evolution 200 Series User Guide Thermo Scientific...

  • Page 465: Chapter 6 Performance Verification

    Use Performance Verification to set up and run tests check the performance of the instrument. Click here information on running the tests. Test results are displayed in reports that are saved automatically and can be opened later (see Data). Thermo Scientific Evolution 200 Series User Guide...

  • Page 466: Pv Test Descriptions

    542 nm. This line is a property of the lamp and serves as a fundamental wavelength standard that does not require calibration. The wavelength and tolerance values cannot be changed. Use an empty cell holder. Evolution 200 Series User Guide Thermo Scientific...
  • Page 467 Use an empty cell holder. Photometric Drift Measures the absorbance drift of the instrument over a one-hour period. Use an empty cell holder and let the instrument warm up fully before initiating this test. Thermo Scientific Evolution 200 Series User Guide...

  • Page 468: Running Pv Tests

    CAUTION Avoid pinch hazard. Keep hands and objects clear of the accessory during operation. Languages ATTENTION Évitez tout risque de pincement. Gardez vos mains, ainsi que tout objet, à l’écart de l’accessoire pendant l’initialisation. Evolution 200 Series User Guide Thermo Scientific...
  • Page 469 Completed PV reports are saved automatically and appear on the PV Reports tab in My Data. See My Data for instructions for viewing results later. 6. Click Close. Related Topics Overview of PV PV Test Descriptions Thermo Scientific Evolution 200 Series User Guide...
  • Page 470 Performance Verification Running PV Tests Evolution 200 Series User Guide Thermo Scientific...

  • Page 471: Chapter 7 Insight Security Software

    • The root directory for template groups shown on the Preference tab in Options is set using Thermo Security Administration. • A Sign button appears in the Performance Verification report window. Use it to sign the displayed report. Related Topics Signing a File Verifying a Signature Thermo Scientific Evolution 200 Series User Guide...

  • Page 472: Signing A File

    1. Choose Security (menu) > Sign > Template File. 2. Locate and select the desired file and then click Open. 3. Enter a password. 4. Select a signature reason (if permitted). 5. Choose OK. A verification message appears. 6. Choose OK. Evolution 200 Series User Guide Thermo Scientific...

  • Page 473: Verifying A Signature

    Security Administration User’s Guide for instructions for using the Access Control features. See “Setting system policies for the client application” in that chapter for instructions for using the System Policies features. Thermo Scientific Evolution 200 Series User Guide...
  • Page 474 Specifies which users can use the commands in the Math menu. Analyze menu Specifies which users can use the commands in the Analyze menu. Ability to set Rate Specifies which users can set parameters in Settings for the parameters Rate application. Evolution 200 Series User Guide Thermo Scientific...
  • Page 475 Prevents users in the specified policy groups from printing data spectral data that has not been saved. Prevent changing Prevents users in the specified policy groups from changing directories when saving files directories (“browsing”) when saving files. Thermo Scientific Evolution 200 Series User Guide...
  • Page 476 Save Workbook or Save As Workbook in the File menu. Directory for templates Designates for each policy group a directory for saving templates with Save Workbook Settings as Template in the File menu. Evolution 200 Series User Guide Thermo Scientific...

  • Page 477: Assigning Signature Meanings

    Security Administration User’s Guide for general instructions for using the Signature Meanings features to change the available meanings or to specify which users can select each meaning when signing a file. Use the signature meanings in the default list as follows. Thermo Scientific Evolution 200 Series User Guide...
  • Page 478 Reviewed - record contents have Indicates that the user signing the file has reviewed it. been reviewed Revision - the record has been Indicates that the user signing the file has changed it. revised Evolution 200 Series User Guide Thermo Scientific...
  • Page 479 Protein Bradford and Smart Sipper sampling Proteins & Labels and VERSA Probe sampling Quant air, for instrument purge Rate alarm Scan acoustic Smart Sipper settings water level 301, Temperature Probe settings Thermo Scientific Evolution 200 Series User Guide...
  • Page 480 CVC calibration data Calibrated Validation Carousel bandwidth 42, 46, 65, 70, 143, 154, 165, 174, 184, 195, 206, calibrating 217, instrument wavelength Bandwidth setting Smart Linear 8-Cell Changer Evolution 200 Series User Guide Thermo Scientific...
  • Page 481 356, touchscreen rotatable computer standard reference connector standard sample external variable pathlength rectangular remote variable pathlength slide switching between local and remote water thermostatted sample computer control Thermo Scientific Evolution 200 Series User Guide...
  • Page 482 CVC calibration control limits date conventions decimal digits Convert Spectra default values copying life science applications CVC reports quantitative analysis results default workbook settings cord deleting routing to sample compartment group 113, Evolution 200 Series User Guide Thermo Scientific...
  • Page 483 63, 183, 194, 205, 216, radiation hazard in detector compartment duplicates routine maintenance dust storing precautions for troubleshooting Thermo Scientific Evolution 200 Series User Guide...
  • Page 484 Smart Sipper Help installing in Smart Sipper organization installing screw-cap Help menu replacing tubing Hg lamp tubing materials access cover fluid connector 109, external connectors description internal connectors Evolution 200 Series User Guide Thermo Scientific...
  • Page 485 Smart Peltier Linear Cell Changer lifting or moving Smart Rotary Cell Changer Local Control INSIGHT Security 457–459, major components INSIGHT software operating precautions for Evolution 200 series spectrophotometer ordering parts INSIGHT window performance verification 451–452, navigation pane removable panels right pane removing detector...
  • Page 486 Smart Peltier Linear Cell Changer Power and Smart Rotary Cell Changer and Stop button Stop Local Control instrument 101, Zero/Base cleaning touchscreen kinetics keyboard data acquisition setting up 128–129 switching INSIGHT software to Evolution 200 Series User Guide Thermo Scientific...
  • Page 487 Pierce BCA installing Pierce Modified Lowry removing Protein A280 storing Protein Biuret moisture Protein Bradford precaution for CVC Proteins & Labels precaution for Fiber Optic Module Quant precaution for instrument Rate Thermo Scientific Evolution 200 Series User Guide...
  • Page 488 Nucleic Acid panel Accessories tab for external tubing connectors application 139, 145–146 sample compartment front Instrument tab sample compartment side Measurement tab overview panels Samples tab removable Type tab parts nucleic acid ordering Evolution 200 Series User Guide Thermo Scientific...
  • Page 489 Pierce BCA 179, 186–187 voltage setting Accessories tab water level alarm Instrument tab water level failure Measurement tab Samples tab Peltier Single Cell Holder Standards tab changing fuse Type tab cleaning Thermo Scientific Evolution 200 Series User Guide...
  • Page 490 Smart Sipper 410–411 probes, fiber optic chemical compatibility aligning installing attaching replacing compatible specifications VERSA Probe purge properties of spectrum and subambient temperature samples protecting explosion hazard Evolution 200 Series User Guide Thermo Scientific...
  • Page 491 8 degree near normal specular data acquisition grazing angle data acquisition parameters integrating sphere exporting results from Instrument tab region Measurement tab finding peaks in Name and Type tab limiting Rate Calculations tab region-threshold tool Thermo Scientific Evolution 200 Series User Guide...
  • Page 492 Run button cleaning connector fluid ports installing manual accessory in safety installing Smart Accessory in Evolution 200 Series User Guide Thermo Scientific...
  • Page 493 Settings button volatile, corrosive or caustic sharing custom templates Samples tab shielding Fixed for USB devices Nucleic Acid shipping cost for warranty repair Nucleic Acid Labels signal-to-noise ratio Pierce 660 nm Protein Assay Thermo Scientific Evolution 200 Series User Guide...
  • Page 494 Smart Peltier Linear Cell Changer cleaning flow cells calibrating compatible flow cells Cell Changer settings description cell changer status monitor installing changing fuse installing flow cell cleaning installing pump tubing compatible cells maintaining components operating precautions Evolution 200 Series User Guide Thermo Scientific...
  • Page 495 Smart Accessories zooming in on or moving installing thermostatted accessories Thermo Scientific Evolution 200 Series User Guide...
  • Page 496 Fiber Optic Module specifications Hg Lamp troubleshooting instrument detector temperature range nanoCell for Peltier Recirculator Smart Sipper 410–411 for Peltier Single Cell Holder stray light for Smart Peltier Linear Cell Changer Evolution 200 Series User Guide Thermo Scientific...
  • Page 497 Sipper pump thermostatted accessory tubing, flow cell installing tubing connections flanged thermostatted sample cell holder low-volume fluid controlled PTFE Peltier controlled replacing thin film sample holder tubing, pump time chemical compatibility tips installing Thermo Scientific Evolution 200 Series User Guide...
  • Page 498 UV radiation hazard 113, 115, 265, valleys warranty finding and failure to use sample compartment windows value crossings items not covered by Value Level period repair Variable Angle Transmission accessory Evolution 200 Series User Guide Thermo Scientific...
  • Page 499 4, 21, opening saving signed signing verifying signature for xenon lamp status Y-axis unit Zero button zero scan initiating from instrument keypad Zero/Base keypad button z-height and nanoCell cell holder for 15 mm spectrophotometer Thermo Scientific Evolution 200 Series User Guide...
  • Page 500 Index: Z Evolution 200 Series User Guide Thermo Scientific...

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