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3.3
CHAMBER COVER
The chamber cover must be closed before a run can begin.
It is not possible to open the cover during a run because of the vacuum in the
concentrator chamber. In addition, a cover lock is present on 220 and 230 volt
models. The cover is locked at all times during a run and whenever power to the
unit is interrupted.
The cover lock is a safety feature that further reduces the risk of injury or damage
from the spinning rotor. Do not attempt to bypass the cover lock to conduct a
run with an open cover.
To remove samples in the event of power failure, insert, at an upward 45° angle,
the lid opening tool (provided with unit) into the vertical slot at the base of the
front of the unit. Firmly raise the handle to a horizontal position and lift up the
lid.
3.4
SIMPLE SYSTEM INTEGRITY TEST
You can use this simple system integrity test to periodically verify the operational
integrity of the vacuum pump and refrigerated vapor trap.
1. Install a clean, dry glass condensation flask.
2. Allow the refrigerated vapor trap to operate for 45 minutes.
3. Open the chamber lid and remove the rotor.
4. Fill a plastic beaker that will fit in the chamber with 50 ml of water and place it
in the chamber. Close the lid.
5. Press the drying rate switch to the "LOW" position.
6. Start the system by pressing the concentrator switch to the "ON" position.
7. After fifteen minutes of operation press the concentrator switch to the "OFF"
position.
8. Immediately open the lid and measure the water sample temperature.
A properly functioning system will bring the sample down in temperature within
the range of 3 to 7° C (33–37° F).
4.0
APPLICATION INFORMATION
110
The ISS
SpeedVac Concentrator is suitable for drying or concentrating prima-
rily aqueous based samples. In addition, the small amount of ethanol
commonly dried from preparations stemming from DNA precipitations can also
be processed with this system.
3
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