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1
Using a Direct Insertion Probe (DIP)
Analyzing Cholesterol with the Ion Trap Series
Step 6: Prepare the Sample
Step 7: Create a Method File
12
Ion Trap and DSQ Series Direct Probe System User's Guide
3. Press Enter when you see the desired number. This loads the method.
1. Prepare the sample cup holder and sample cup.
a. Place the sample cup holder on a level surface.
b. Insert a clean sample cup into the sample cup holder.
2. Place sample in the sample cup using a 10 μL syringe to place 1 μL of the 10 μg/mL
cholesterol solution on the bottom of the sample cup.
3. Wait several minutes for all of the solvent to evaporate. It takes several minutes to
evaporate the solvent, giving you ample time to set up a method and a sequence file
("Step 7: Create a Method File"
page
13).
4. Place the sample cup onto the DIP tip using forceps to insert the sample cup onto the
DIP tip.
1. Create a method using Instrument Setup.
a. From the Windows desktop, click the Xcalibur icon to display the Xcalibur Home
Page.
b. Select Instrument Setup to display the Instrument Setup window.
2. Enter the instrument method parameters.
a. Select File | New to create a new method.
1
X refers to methods numbered 1 - 9.
on
page 12
and
"Step 8: Create a Sequence File"
on
Thermo Scientific
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