Prepare Gel; Sample Loading Guidelines - Thermo Scientific invitrogen E-Gel G8121ST User Manual

Prepare gel

1. Remove E-Gel
2. Gently remove comb from the cassette.
3. Load the gel into the cassette compartment, starting
from the right edge.
4. Press down on the left side of the cassette to secure
the cassette.
5. Load gels within 15 minutes after opening the
package.

Sample loading guidelines

Use the recommended total loading volume for each gel type. Do not load more than
•
recommended amount of DNA sample or ladder per well.
Load deionized water into all empty wells.
•
• Keep all sample volumes uniform. If you do not have enough samples to load all the wells of
the gel, load an identical volume of deionized water into any empty wells. Prepare your
samples by adding E-Gel
amount of DNA to bring the total required sample volume.
Avoid introducing bubbles while loading. Bubbles can cause band distortion.
•
E-Gel
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E-Gel
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E-Gel
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E-Gel
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E-Gel
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E-Gel
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E-Gel
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16
agarose gel from package.
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1X Sample Loading Buffer or deionized water to the required
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Gel type
EX
with SYBR Safe
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EX Double Comb
EX Double Comb with SYBR
CloneWell II
SizeSelect II
NGS
Safe
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E-Gel Power Snap Electrophoresis System User Guide
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Total loading volume
20 μL
25 μL
25 μL
20 μL