Sample Application - Thermo Scientific Vitrobot Mk IV User Manual

Chapter | Operating Instructions
When the ethane/propane container is ready for vitrification the holder can be placed on the
platform-ring under the Vitrobot.
Note Make sure that liquid nitrogen remains present in the peripheral ring throughout the
entire vitrification procedure.
Note Make sure that the metal spindle is removed from the coolant container prior to the
vitrification procedure.
Note Working with explosive materials like ethane and propane requires working in a
spark free-fume hood!
After the coolant container has been placed on the platform-ring, the foot pedal switch or the
'Continue' button in the User Interface must be pressed in order to raise the container towards
the bottom of the climate chamber.

3.7 Sample Application

It is possible to skip application if the sample is already applied onto the grid.
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PN 103261 | Revision D | 23-Oct-2019
User Manual
Page 26