Thermo Scientific Applied Biosystems GeneChip System 3000 Manuals

Manuals and User Guides for Thermo Scientific Applied Biosystems GeneChip System 3000. We have 1 Thermo Scientific Applied Biosystems GeneChip System 3000 manual available for free PDF download: Operation And Use

Thermo Scientific Applied Biosystems GeneChip System 3000 Operation And Use

Thermo Scientific Applied Biosystems GeneChip System 3000 Operation And Use (74 pages)

Brand: Thermo Scientific | Category: Laboratory Equipment | Size: 7 MB

Table of Contents

Table of Contents
3
CHAPTER 1 Introduction

7
- Product Overview
  7
- Instrument Usage Information
  7
- Other Documentation
  7
- Terminology
  7
- Alerts
  8
- Warnings and Precautions
  8
- General Chemical Safety
  9
- Biological Hazard Safety
  9
- Fluid Safety
  10
- Equipment Safety
  10
- Disposal Information and Guidance
  10
- Precautions
  10
- Customer Support
  11
CHAPTER 2 System Overview

12
- Hardware
  12
- Standard Hardware Components
  12
- Fluidics Station 450
  13
- Scanner 3000 with Autoloader
  13
- Genechip Hybridization Oven 645I
  13
- Optional Hardware Components
  13
- Software
  14
- Gcdc
  14
- Library and Fluidics Protocol Files
  14
- GCDC Launcher
  14
- Launcher Modules
  15
- GCDC Workflow Overview
  15
- How GCDC Processes Arrays
  15
- How GCDC Processes Cartridge Arrays
  15
- Registration
  16
- Registering the Samples and Arrays
  16
- Registering Sample Options
  16
- Editing User Attributes
  16
- Washing and Staining Cartridge Arrays
  16
- Running the Scanners
  16
- Tracking Gridding and CEL File Generation
  16
- GCDC File Types
  17
- Sample Files
  17
- Log Files
  17
- Customer Support
  17
CHAPTER 3 System Operation Basics

18
CHAPTER 4 Genechip Fluidics Station FS450

20
- Before You Begin
  20
- Warnings and Precautions
  20
- When to Contact Support
  21
- About the Instrument
  21
- Modules
  22
- Components
  22
- Connections
  24
- Using the Fluidics Station 450
  24
- Assay Documentation
  24
- Fluidics Station 450 Protocols
  24
- Fluidics Station Operation
  25
- Starting the Fluidics Station
  25
- Priming Fluidics Station
  25
- Priming the Fluidics Station
  25
- Fluidics Control
  26
- Fluidics Control Window
  27
- Master Controls
  27
- Station Controls
  27
- Status Window
  28
- Running Protocols
  29
- Priming the Fluidics Station
  29
- Running a Fluidics Station Protocol
  30
- Shutting down the Fluidics Station
  33
- Fluidics Station Care and Maintenance
  35
- Instrument Care
  35
- Fluidics Station Bleach Protocol
  35
- Bleach Cycle
  36
- Rinse Cycle
  38
- Peristaltic Tubing Maintenance
  40
- Replacing the Peristaltic Tubing
  40
- Fluidics Station Troubleshooting and Assistance
  42
- Troubleshooting Decision Tree
  42
- Problems and Solutions
  44
- Error Message Meanings
  46
- Other Problems and Solutions
  47
- When to Contact Support
  48
- Fluidics Station LCD Messages
  48
CHAPTER 5 Genechip Scanner 3000 with Autoloader

50
- Before You Begin
  50
- About the Instrument
  50
- Safe Operation
  51
- Precautions
  51
- Laser Safety
  51
- Location of Safety Symbols
  52
- Electrical Safety
  54
- Connections
  55
- Workstation: Rear Connections
  55
- Scanner: Rear Connections
  56
- Scanner Indicator Lights and On/Off Button
  57
- Buttons and Indicators
  57
- Indicator Lights Summary Table
  58
- Scanning Arrays
  58
- Use of Tough-Spots™ to Prevent Leaks
  58
- Loading Arrays into Carousel
  59
- Starting the Scanner
  61
- Shutting down the Scanner
  61
- Using GCDC with the Autoloader Disabled
  61
- Cleaning and Maintenance
  63
- Troubleshooting
  63
- Scanner Troubleshooting Table
  63
- Scanner Operation Issues Table
  64
- Scanner Diagnostic Table
  64
- Scanner Error Messages
  65
- Removing a Lodged Array Cartridge Manually
  65
- When to Contact Support
  69
APPENDIX A Specifications and Labeling Information

70
- Specifications
  70
- Genechip Fluidics Station 450
  70
- Scanner
  70
- Hybridization Oven
  71
- Labeling Symbols
  72
APPENDIX B Collecting Log Files

73
- Using the Log Collector
  73

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