Checking The Gas Supplies; Checking The Esi Fused-Silica Sample Tube For Elongation; After Operating The Ltq Series System - Thermo Scientific LTQ Series Hardware Manual

4
Daily Operation

After Operating the LTQ Series System

Checking the Gas Supplies

Checking the ESI Fused-Silica Sample Tube for Elongation

After Operating the LTQ Series System
48 LTQ Series Hardware Manual
Check the helium gas supply on the regulator of the gas tank. Make sure that there is
sufficient gas for the analysis, which is a minimum of 3447 kPa (500 psi) on the high pressure
gauge of the regulator. If necessary, replace the tank. Verify that the pressure of helium
reaching the mass spectrometer is 275 ±70 kPa (40 ±10 psi). If necessary, adjust the pressure
with the tank pressure regulator.
Check the nitrogen gas supply on the regulator of the nitrogen gas tank or liquid nitrogen
boil-off tank. Make sure that there is sufficient gas for the analysis. If necessary, replace the
tank. Verify that the pressure of nitrogen reaching the mass spectrometer is 689 ±138 kPa
(100 ±20 psi). If necessary, adjust the pressure with the tank pressure regulator.
Note When operating 24 hours a day and 7 days a week, typical daily consumption of
nitrogen gas is 5560–26700 L (196–943 ft
For more information about gas requirements, including those for a MALDI LTQ XL system
and the ETD reagent carrier gas for the LTQ XL/ETD system, refer to the LTQ Series
Preinstallation Requirements Guide.
Using acetonitrile in the mobile phase can elongate the polyimide coating on the fused-silica
sample tube, which can degrade both signal intensity and stability over time.
If you are using a fused-silica sample tube in the ESI mode, verify that the sample tube is not
elongated past the tip of the ESI spray needle. If the tube is elongated, cut and reposition it.
See "Trimming the ESI Sample Tube"
Follow these preventive maintenance procedures every day after operating the LTQ Series
system:
• Flushing the Sample Transfer Line, Sample Tube, and API Probe
• Cleaning the Ion Sweep Cone, Spray Cone, and Ion Transfer
• Purging the Oil in the
• Emptying the Solvent Waste
• Placing the System in Standby
3
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on page 58.
Forepump, on page 51
Container, on page 52
Mode, on page 52
Tube, on page 51
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