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3.1.4
Turn On the Lamps
To turn on the lamps, click UV Lamp and VIS Lamp on the Chromeleon
7 ePanel or the Chromeleon 6.8 Control panel.
When a detector lamp is turned on for the first time after the detector
power is turned on, wavelength calibration is performed automatically.
Calibration starts shortly after the lamp is ignited. The 0-order radiation is
checked. The 0-order position is where all light is reflected off the grating
and no diffraction occurs; this position is thus used as the "home" position
for the grating.
NOTE If you want the lamps to be turned on automatically
3.2
System Equilibration
Before you can begin using the detector for sample analysis, the chromatography
system must be equilibrated. To equilibrate the system, perform the following
tasks:
•
Pump the starting solvent through the entire system until the system is free of
any other liquid composition.
•
Heat or cool all temperature-controlled devices (for example, a column
compartment) to the temperature required for the application.
•
Set the detector wavelength(s) and turn on the lamp(s). (Wavelength
calibration occurs automatically at this point.)
NOTE To achieve optimum results, allow 60 minutes for the
•
Monitor the pump pressure and verify that the reading is correct for the
application and is stable.
•
Monitor the detector signal and verify that the baseline signal is at the
expected reading for your application and is stable. This may not be the case if
the solvent composition has been modified, or if air bubbles exist in the light
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at startup, execute the AutoactivateUV_Lamp=On
and AutoactivateVisible_Lamp=On commands in
the Chromeleon 7 Command window or the
Chromeleon 6.8 Commands dialog box.
lamp(s) to stabilize before beginning operation.
3 • Operation and Maintenance
29
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