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4
Operation
Rotor Temperature Equilibration
Rotor Temperature Equilibration
To equilibrate the rotor temperature in the centrifuge chamber:
1. Install the rotor in the rotor chamber, place empty buckets (if applicable) in all positions, and the
rotor cover, if any.
2. Close the door.
3. Turn off all options (access with MENU key).
4. Check to be sure the correct rotor name is in the ROTOR field of the SET DISPLAY. If not, press
ROTOR until it is.
5. Press SPEED. If cooling, input 1500 rpm. If heating, input a value that is approximately 70% of
the maximum rated speed of the selected rotor.
6. Press HOLD to select a continuous run.
7. Press TEMP and enter the desired temperature.
8. Press START to begin temperature equilibration.
9. The centrifuge will alert you that a sample overtemperature condition exists, and that pre-cooling
is taking place. Press CLEAR.
10. Monitor progress of the value in the RUN TEMPERATURE display, and note the time when the
displayed RUN temperature reaches the SET temperature. From the time it does, allow the run to
continue for an additional 30 minutes, then press STOP.
4-14
RC B
IOS
Thermo Scientific
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