Installing The Column In The Gc Inlet - Thermo Scientific TSQ 9000 Hardware Manual

1
Performing Routine Maintenance
Replacing a Column

Installing the Column in the GC Inlet

28 TSQ 9000 Hardware Manual
1. Remove the current column:
a. Open the front door of the GC.
WARNING BURN HAZARD: The injector, oven, and transfer line may be hot.
Allow them to cool to room temperature before touching them.
AVERTISSEMENT RISQUE DE BRÛLURE : l'injecteur, le four et la ligne de transfert
peuvent être chauds. Laissez-les refroidir à la température ambiante avant de les
toucher.
b. Unscrew the transfer line nut and remove the column from the transfer line.
c. Unscrew the injector and detector nuts and remove the column.
d. Remove the column from the column rack and from the GC.
2. Connect the new column to the injector inside the GC.
Note Wear clean, lint- and powder-free gloves when you handle the column and
injector ferrule.
a. Unwind the column enough to easily connect its ends to the injector and detector.
b. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
c. Insert the column through the injector retaining nut and ferrule (larger end up). If
the M4 retaining nut is used, slide it on the column through the side cut. Wipe the
column again with a tissue soaked in methanol.
Tip Slide a notched septum on the column before the injector retaining nut to
make it easier to measure the proper distance between the nut and end of the
column.
d. Use a scoring wafer to score and break the column about 1 cm (0.4 in.) from the end.
Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
e. Insert a notched septum on the column to hold the retaining nut at this position.
Thread the retaining nut into the injector but do not tighten.
f. Ensure that the end of the column is the proper distance (splitless = 5 mm,
split = 10 mm, PTV and PTVBKF = 30 mm) from the back of the injector nut.
g. Adjust the column position so that the septum contacts the bottom of the retaining
nut. Use your fingers to tighten the retaining nut until it starts to grip the column.
h. Tighten the column nut finger-tight until it starts to grip the column plus a quarter
turn.
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