Thermo Scientific Dionex ICS-5000 Operator's Manual page 126

Dionex ICS-5000 Operator's Manual
time (in minutes) is on the x-axis and the detector response (in
nanoCoulombs) is on the y-axis.
40
Response
(nC)
0
0
Figure 2-56. Example Chromatogram for a Pulsed Amperometry Application
For 3D amperometry data, Chromeleon stores raw data points at a rate of
1 KHz throughout the entire run. Each raw 3D data point is defined by the
following attributes: the detector current (1) recorded at a particular
waveform time (2) and at a specific retention time (3). As a result, the two-
dimensional view of an integrated amperometry chromatogram (retention
time vs. integrated current) is extended by a third dimension (waveform time).
Thus, data for the entire waveform period—not just the integration
intervals—are collected.
Displaying and Reprocessing 3D Data
This section describes how to display and reprocess 3D amperometry data
using Chromeleon 6.8. If you are using Chromeleon 7, the features described
here are available by displaying the data in a contour plot. For details, refer to
the Chromeleon 7 Help.
In Chromeleon 6.8 you can display and reprocess 3D amperometry data in the
3D Amperometry View window (see
110
Analytical Column: CarboPac PA10
Eluent:
Flow Rate:
Detection:
Electrode:
Waveform:
2
Peaks:
1
3
4
5
Retention Time (Minutes)
Minutes
Figure 2-57). To open the window,
(4 x 250 mm) and Guard
18 mM NaOH
1.5 mL/min
Pulsed Amperometry
Gold
Quadruple Potential
1. L-Fucose
2. D-Galactosamine
3. D-Glucosamine
4. D-Galactose
5. D-Glucose
5
6. D-Mannose
6
10
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