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ABL800 FLEX Operator's Manual
Sampling procedures,
Sampling with a
Prepare the following items:
test tube
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Step
Pleural liquid
To collect a pleural liquid sample, do the following:
sampling
Step
Continued
A test tube and cap
Anticoagulant: lithium or sodium heparin (1000 IU per mL sample)
Pipette or syringe and a needle for heparin dosage
One hypodermic needle, 20-25 gauge
Tourniquet
Skin antiseptic, e.g. 70 % alcohol
Sterile gauze sponge or cotton wool
Adhesive tape and pressure dressing
Action
Transfer the heparin to the test tube using a syringe or a pipette: 50 μL
1.
heparin per 5 mL blood.
2.
Extend the patient's arm. Apply the tourniquet for a venous puncture.
3.
Locate the vein with the fingertip. Clean the skin area with antiseptic
and a sterile gauze sponge or cotton wool.
4.
Collect the sample according to the procedure in your institution.
Apply dry gauze or cotton wool over the puncture site.
5.
6.
Close the test tube with a cap, then mix the contents by inverting it or
rolling it between your palms about 20 times.
7.
Apply a pressure dressing over the puncture site and elevate the site
for at least 2-3 minutes, if possible.
8.
Mix the sample immediately before analysis. Analyze the sample
immediately.
Action
1.
Collect the sample directly from the patient or from a drain.
NOTICE: Radiometer recommends always to use heparinized
samplers for pleural liquids to avoid introducing clots in the analyzer.
12. Sampling
Continued on next page
12-13
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