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4
Nucleic Acid Applications
Measure Oligo DNA or Oligo RNA
Settings for Oligo DNA and Oligo RNA Measurements
Setting
Available Options
Oligo Base
for DNA: Use the
Sequence
G, A, T and C keys to
specify the DNA base
sequence
for RNA: Use the
G, A, U and C keys to
specify the RNA base
sequence
56
NanoDrop One User Guide
The Oligo setup screen appears after you select the Oligo DNA or Oligo RNA
application from the Nucleic Acids tab on the Home screen. To show the Oligo
settings from the Oligo DNA or Oligo RNA measurement screen, tap
Oligo DNA Setup (or
Description
Specify your DNA or RNA base sequence by tapping the
corresponding keys:
Each time a base is added to the sequence, the software
calculates the following:
• Factor. Constant used to calculate oligonucleotide
Add guanine
Add adenine
Add thymine (DNA) or
uracil (RNA)
concentration in
modified Beer's Law
extinction coefficient and pathlength:
f = 1/(
* b)
260
where:
f= factor
= molar extinction coefficient at 260 nm in ng-cm/µL
b =
sample pathlength
measured with the NanoDrop One instrument)
Oligo RNA Setup).
Remove most
recent base
Add cytosine
equation. Based on
in cm (0.1 cm for nucleic acids
Thermo Scientific
>
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