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6
Measure OD600
Setting
Additional monitored
wavelength (
)
Cell number
conversion factor
8
(10
)
142
NanoDrop One User Guide
Available Options
Any wavelength
between 250 nm and
700 nm
Any number
Related Topics
•
Instrument Settings
Description
User-defined wavelength. Enter an additional wavelength
to measure if desired (useful for dilute samples that exhibit
low absorbance at 600 nm).
If an alternative wavelength is specified, use this equation
to calculate cell concentration:
c = A
* factor
(
)
where:
c = analyte concentration in cells/mL
A(
) = UV-visible absorbance at specified wavelength in
absorbance units (A)
factor(
) = 1/(
(
) * b) in mL/cell-cm
where:
(
) = molar absorption coefficient (or extinction
coefficient) at specified wavelength
b = pathlength in cm (1.0 cm for the NanoDrop One
instruments)
User-defined factor. Generally accepted factor for
measured cell type, or one derived empirically using a
solution of study cells at known concentration using the
same media.
8
Default value is 1x10
factor for most bacterial cell suspensions such as E. coli.
Tip: The factor is wavelength specific for each cell type
and can be affected by the type of media used for the
measurements. Ideally, the factor should be determined
empirically using a solution of the study cells at a known
concentration using the same media.
(
)
which is the generally accepted
Thermo Scientific
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