Thermo Scientific NanoDrop One User Manual page 64

5 Protein Applications
Measure Protein A280
62 NanoDrop One User Guide
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
Pedestals.
To measure a Protein A280 sample
1. From the Home screen, select the Proteins tab and select Protein A280.
2. Specify a sample type
3. Pipette 1–2 µL blanking solution onto the lower pedestal and lower the arm, or
insert the blanking cuvette into the cuvette holder.
Tip: If using a cuvette, make sure to
instrument light path.
4. Tap Blank and wait for the measurement to complete.
Tip: If Auto-Blank
lower the arm. (This option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new laboratory wipe, or remove the
blanking cuvette.
6. Pipette 2 µL sample solution onto the pedestal and lower the arm, or insert the
sample cuvette into the cuvette holder.
7. Start the sample measurement:
– Pedestal: If Auto-Measure
and tap Measure.
– Cuvette: Tap Measure
When the sample measurement is completed, the spectrum and reported values
are displayed (see the next section).
8. When you are finished measuring samples, tap End Experiment.
9. Lift the arm and clean both pedestals with a new wipe, or remove the sample
cuvette.
and baseline correction
align the cuvette light path
is On, the blank measurement starts automatically after you
is On, lower arm; if Auto-Measure is off, lower arm
Cleaning the
if desired.
with the
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