Measure Proteins And Labels; Measure Labeled Protein Samples - Thermo Scientific NanoDrop One User Manual

Measure Proteins and Labels

Measure Labeled Protein Samples

To measure labeled protein samples
Thermo Scientific
Measures the concentration of purified
proteins that have been labeled with up
to two fluorescent dyes.
Measure Labeled Proteins
Reported Results
Settings
Detection Limits
Calculations
Use the Proteins and Labels application to quantify proteins and fluorescent dyes for
protein array conjugates, as well as metalloproteins such as hemoglobin, using
wavelength ratios. This application reports protein concentration measured at
280 nm, an A269/A280 absorbance ratio, and the concentrations and measured
absorbance values of the dyes, allowing detection of dye concentrations as low as
0.2 picomole per microliter. This information is useful for evaluating protein/dye
conjugation (degree of labeling) for use in downstream applications.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
Before you begin...
Before taking pedestal measurements with the NanoDrop One instrument, lift the
instrument arm and clean the upper and lower pedestals. At a minimum, wipe the
pedestals with a new laboratory wipe. For more information, see
Pedestals.
To measure a labeled protein sample
1. From the Home screen, select the Proteins tab and then tap Protein &
Labels.
5
Protein Applications
Measure Proteins and Labels
Cleaning the
NanoDrop One User Guide 85