Best Practices For Protein Measurements - Thermo Scientific NanoDrop One User Manual

Best practices for protein measurements

Thermo Scientific
High concentration BSA sample
Low concentration BSA sample
• Isolate and purify protein samples before measurement to remove impurities.
Depending on the sample, impurities could include DNA, RNA and some buffer
components. See Preparing Samples
Note Extraction reagents that contribute absorbance between 200 nm and
280 nm will affect measurement results if present in samples (even residual
amounts).
• Ensure the sample absorbance is within the instrument's
limits.
5
Protein Applications
for more information.
absorbance detection
NanoDrop One User Guide
Measure Protein A280
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