Best Practices For Uv-Vis Measurements - Thermo Scientific NanoDrop One User Manual

Best practices for UV-Vis measurements

Thermo Scientific
3. Pipette 1–2 µL blanking solution onto the lower pedestal and lower the arm, or
insert the blanking cuvette into the cuvette holder.
Tip: If using a cuvette, make sure to
instrument light path.
4. Tap Blank and wait for the measurement to complete.
Tip: If Auto-Blank
lower the arm. (This option is not available for cuvette measurements.)
5. Lift the arm and clean both pedestals with a new laboratory wipe, or remove the
blanking cuvette.
6. Pipette 1-2 µL sample solution onto the pedestal and lower the arm, or insert the
sample cuvette into the cuvette holder.
7. Start the sample measurement:
– Pedestal: If Auto-Measure
and tap Measure.
– Cuvette: Tap Measure.
When the sample measurement is completed, the spectrum and reported values
are displayed (see the next section).
8. When you are finished measuring samples, tap End Experiment.
9. Lift the arm and clean both pedestals with a new wipe, or remove the sample
cuvette.
• Ensure the sample absorbance is within the instrument's
limits.
• Blank with the same buffer solution used to re-suspend the analyte of interest.
The blanking solution should be a similar pH and ionic strength as the analyte
solution.
• Run a blanking cycle
solution. If the buffer exhibits strong absorbance at or near an analysis
wavelength, you may need to choose a different buffer or application. See
Choosing and Measuring a Blank
• For micro-volume measurements:
– Ensure pedestal surfaces are properly
– Ensure samples are homogeneous before taking a measurement. Avoid
introducing bubbles when mixing and pipetting.
– Follow best practices for micro-volume measurements.
align the cuvette light path
is On, the blank measurement starts automatically after you
is On, lower arm; if Auto-Measure is off, lower arm
to assess the absorbance contribution of your buffer
for more information.
7
Custom Applications
with the
absorbance detection
cleaned and conditioned.
NanoDrop One User Guide
Measure UV-Vis
147