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10
Maintenance
Maintaining the Pedestals
Reconditioning the Pedestals
256
NanoDrop One User Guide
The pedestal surfaces may lose their "conditioned" properties over time, especially
after measurements with isopropyl alcohol or solutions that contain surfactants or
detergents such as the
droplets on the lower pedestal to "flatten out," preventing proper formation of the
liquid column when the arm is lowered. The resulting spectrum may look "rough" or
"jagged."
If samples flatten out on the pedestal (rather than "beading up" or forming a rounded
droplet) or the liquid column breaks during a measurement, recondition the
pedestals.
Unconditioned pedestal
(droplet flattens out)
Supplies needed
• lint-free laboratory wipes
•
PR-1 pedestal reconditioning kit
• calibrated precision pipettor (0-2 µL)
• canned air
Bradford
reagent. An unconditioned pedestal causes
Properly conditioned pedestal
(droplet beads up)
(available from us or a local distributor)
Thermo Scientific
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