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5
Protein Applications
Measure Protein A280
Setting
Available Options
IgG
Lysozyme
Other protein
(
+ MW)
Other protein
(
1%)
a
To add or edit a custom protein, use Protein Editor.
68
NanoDrop One User Guide
Mass Ext.
Coefficient
(L/gm-cm)
13.7
26.4
User entered molar
extinction coefficient
and molecular
weight
User entered mass
extinction coefficient
Description
Suitable for most mammalian antibodies (i.e.,
immunoglobulin G or IgG). Calculates protein
concentration using mass extinction
coefficient (
) of 13.7 L/gm-cm at 280 nm for
1% (i.e., 10 mg/mL) IgG solution. Assuming
MW is 150,000 Da, molar extinction coefficient
at 280 nm for IgG is approximately
-1
-1
210,000 M
cm
.
Calculates lysozyme protein concentration
using mass extinction coefficient (
26.4 L/gm-cm at 280 nm for
1% (i.e., 10 mg/mL) lysozyme solution.
Assumes molar extinction coefficient for egg
white lysozyme ranges between
-1
-1
36,000 M
cm
and 39,000 M
Assumes protein has known molar extinction
coefficient (
) and molecular weight (MW),
where:
(
)*10=(
)*(MW
molar
percent
Enter MW in kiloDaltons (kDa) and molar
extinction coefficient (
1000 (i.e.,
/1000). For example, for protein
with molar extinction coefficient of 210,000
-1
-1
M
cm
, enter 210.
Assumes protein has known mass extinction
coefficient (
). Enter mass extinction
coefficient in L/gm-cm for 10 mg/mL (
protein solution.
) of
-1
-1
cm
.
)
protein
-1
-1
) in M
cm
divided by
1%)
Thermo Scientific
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